Connection between Telomerase Activity in PBMC and Markers of Inflammation and Endothelial Dysfunction in Patients with Metabolic Syndrome

Abstract

Metabolic syndrome (MS) is a constellation of metabolic derangements associated with vascular endothelial dysfunction and oxidative stress and is widely regarded as an inflammatory condition, accompanied by an increased risk for cardiovascular disease. The present study tried to investigate the implications of telomerase activity with inflammation and impaired endothelial function in patients with metabolic syndrome. Telomerase activity in circulating peripheral blood mononuclear cells (PBMC), TNF-α, IL-6 and ADMA were monitored in 39 patients with MS and 20 age and sex-matched healthy volunteers. Telomerase activity in PBMC, TNF-α, IL-6 and ADMA were all significantly elevated in patients with MS compared to healthy volunteers. PBMC telomerase was negatively correlated with HDL and positively correlated with ADMA, while no association between TNF-α and IL-6 was observed. IL-6 was increasing with increasing systolic pressure both in the patients with MS and in the healthy volunteers, while smoking and diabetes were positively correlated with IL-6 only in the patients' group. In conclusion, in patients with MS characterised by a strong dyslipidemic profile and low diabetes prevalence, significant telomerase activity was detected in circulating PBMC, along with elevated markers of inflammation and endothelial dysfunction. These findings suggest a prolonged activity of inflammatory cells in the studied state of this metabolic disorder that could represent a contributory pathway in the pathogenesis of atherosclerosis.

Dietary supplementation with tomato-juice in patients with metabolic syndrome: A suggestion to alleviate detrimental clinical factors

“…Asymmetric dimethyl arginine (ADMA) is linked to the impairment of endothelial NO-dependent function, by inhibiting endogenous nitric oxide synthetase in a concentration dependent manner, and has been associated with cardiovascular diseases (Boger et al, 1998;Palomo et al, 2011). The pathophysiology of insulin resistance involves pro-inflammatory cytokines, altered adipokines and endothelial dysfunction (Rentoukas et al, 2011;Tsarouhas et al, 2011). Moreover, dyslipidemia is a major risk factor for development of several obesity-related diseases.…”

Association Of Telomerase Activity With The Prognosis Of Diabetes Mellitus In Saudi Population

“…Their result apparently confirms that threshold effect is related to telomere length and cellular senescence. Another study performed by Rentoukas et al (2012) to reveal the effect of telomerase in patients with inflammation and impaired endothelial function found that patients with metabolic syndromes had significant telomerase activity in their circulating PBMC. These results suggested that prolonged inflammation could act as a pathway in atherosclerosis resulted from diabetes.…”

Telomere length, telomerase activity and mechanisms change in patients with type 2 diabetes mellitus

“…Длительное хроническое воспаление приво-дит к истощению теломеразы, что мы и наблюдали у пациентов с СД2. При менее выраженном и менее длительном хроническом воспалении, как это имеет место при метаболическом синдроме или умерен-ном атеросклерозе, напротив, наблюдается увели-чение активности теломеразы [20], что, вероятно, носит компенсаторный характер, замедляя умень-шение длины теломер в активно делящихся клетках …”