TEGDMA Reduces Mineralization in Dental Pulp Cells

Abstract

A supplemental appendix to this article is published electronically only at http://jdr.sagepub.com/supplemental. ABSTRACT Direct application of dentin bonding agents onto the exposed pulp has been advocated, but in vivo studies indicate a lack of reparative dentin formation. Our objective was to investigate the role of triethylene glycol dimethacrylate (TEGDMA), a commonly used compound in dentin bonding agents, as a potential inhibitor of mineralization. Human pulp cells were exposed to different concentrations of TEGDMA, and expression of the mineralization-related genes collagen I, alkaline phosphatase, bone sialoprotein, osteocalcin, Runx2, and dentin sialophosphoprotein was analyzed. Gene expression studies by real-time polymerase chain-reaction revealed a concentration-and timedependent decrease of mineralization markers. A subtoxic TEGDMA concentration (0.3 mM) reduced expression levels by 5 to 20% after 4 hrs and by 50% after 12 hrs. Furthermore, alkaline phosphatase activity and calcium deposition were significantly lower in dental pulp cells treated with TEGDMA over 14 days. These findings indicate that even low TEGDMA concentrations might inhibit mineralization induced by dental pulp cells, thus impairing reparative dentin formation after pulp capping with dentin bonding agents.

Complete pulpodentin complex regeneration by modulating the stiffness of biomimetic matrix

“…Transcription Kit (Qiangen, USA) from each sample. Human-specific primers were designed and synthesized as follows [39]: collagen a(1) I (sense 5 0 -AAAAGGAAGCTTGGTCC ACT-3 0 ; antisense 5 0 -GTGTGGAGAAAGGAGCAGAA-3 0 ), ALP (sense 5 0 -CCACGTCTTCACATTTGGTG-3 0 ; antisense 5 0 -AGACTGCGCCTGGT AGTTGT-3 0 ), dentin matrix protein 1 (sense 5 0 -TGGGGATTATCCTGT GCTCT-3 0 ; antisense 5 0 -TACTTCTGGGGTCACTGTCG-3 0 ); osteocalcin (sense 5 0 -ACTGTGACGAGTTGGCTGAC-3 0 ; antisense 5 0 -CAAGGGCA AGAGGAAAGAAG-3 0 ) and dentin sialophosphoprotein (sense 5 0 -TT AAATGCCAGTGGAACCAT-3 0 , antisense 5 0 -ATTCCCTTCTCCCTTGTGA C-3 0 ). The primers of thyrotropin-releasing hormone degrading enzyme (TRHDE) (PrimePCR™ PreAmp for Probe Assay: TRHDE, Human) and syndecan3 (SDC3) (PrimePCR™ PreAmp for Probe Assay: SDC3, Human) were purchased from Bio-Rad Laboratories Inc. (Hercules, CA).…”

Magnesium-Containing Nanostructured Hybrid Scaffolds for Enhanced Dentin Regeneration

“…The total RNA was extracted using Trizol (Invitrogen), and the first-strand cDNA was reversetranscribedwithTaqManreversetranscriptionreagents(Applied Biosystems) from each sample. Human-specific primers were designed and synthesized as follows 32 : collagen a(1) I (sense 5¢-AAAAGGAAGCTTGGTCCACT-3¢; antisense 5¢-GTGTG GAGAAAGGAGCAGAA-3¢), ALP (sense 5¢-CCACGTCTT CACATTTGGTG-3¢; antisense 5¢-AGACTGCGCCTGGTA GTTGT-3¢), dentin matrix protein 1 (DMP1; sense 5¢-TGGG GATTATCCTGTGCTCT-3¢; antisense 5¢-TACTTCTGGG GTCACTGTCG-3¢), osteocalcin (OCN; sense 5¢-ACTGTGA CGAGTTGGCTGAC-3¢; antisense 5¢-CAAGGGCAAGAGG AAAGAAG-3¢), and dentin sialophosphoprotein (DSPP; sense 5¢-TTAAATGCCAGTGGAACCAT-3¢, antisense 5¢-AT TCCCTTCTCCCTTGTGAC-3¢). Glyceraldehyde-3-phosphate dehydrogenase (sense 5¢-GAGTCAACGGATTTGGTCGT-3¢; antisense 5¢-GACAAGCTTCCCGTTCTGAG-3¢) and GAPDH (sense 5¢-AGCCGCATCTTCTTTTGCGTC-3¢; antisense 5¢-TCATATTTGGCAGGTTTTTCT-3¢) was used as an internal control.…”

Effects of a dental adhesive on cell cycle regulatory proteins

“…They also down-regulate gene expression in pulp cells and inhibit mineralization thus affecting negatively the regenerative potential of dentin/pulp complex 8,10,24) . HEMA and TEGDMA are very common monomers in dental adhesives.…”