Technology trends in antibody purification

Experiment and simulation of an aqueous two-phase extraction process for the purification of a monoclonal antibody

“…The purity of IgG1 in the cell supernatant amounts to 7.9 % and increases to 93.4 % representing a purification factor of 11.9. For ATPE process configurations C1 and C2 high IgG1 purities are achieved which are close to the range of protein A chromatography [32] with regard to overall impurities and indicate the purification potential of the ATPE process. Host cell protein content by ELISA was not measured at this point.…”

Purification of monoclonal antibodies by chemical affinity mixed mode chromatography

“…Frequently, affinity chromatography has been employed as the preferred separation method at the laboratory or process scale [13] with the traditional ligands based on bacterial Fc receptors, such as Protein A from Staphylococcus aureus, or Protein G from Streptococcus sp, favoured [5,14,15]. The use of such biological ligands, however, has several drawbacks including their overall low productivity [12] and their relatively high cost [13]. In addition, the low pH conditions required to elute the antibody from Protein A resins can damage both the target and ligand proteins and can be incompatible with subsequent downstream steps [12,16].…”

“…Purification of monoclonal antibodies from crude cell culture feedstocks thus requires multi-step processes, which have in the past included membrane-based tangential flow filtration methods, aqueous two phase separation procedures and affinity, ion exchange and hydrophobic interaction chromatography [6][7][8][9][10][11][12]. Frequently, affinity chromatography has been employed as the preferred separation method at the laboratory or process scale [13] with the traditional ligands based on bacterial Fc receptors, such as Protein A from Staphylococcus aureus, or Protein G from Streptococcus sp, favoured [5,14,15].…”

Prediction of the isotherms of human IgG adsorption on Ni(II)-IDA-PEVA membrane using artificial neural networks

“…For instance, when affinity membranes were used, the mass transfer is governed by convection, which facilitates the macromolecule transport to the adsorption site, providing higher flow rates, lower pressure drops, higher productivities and lower costs in several cases (Klein 1991; Thommes and Kula 1995;Bueno et al 1995;Aquino et al 2006;Varadaraju et al 2011;Gagnon 2012).…”