Technology of Production and Control of Xanthomonas Campestris Phage Bioproduct

Abstract

Traditional methods of combating bacterial diseases of plants, including vascular bacteriosis of Cruciferous plants, do not currently allow to achieve effective result, which, among other things, is associated with the ability of phytopathogens to adapt to changing environmental conditions. Application of bacteriophages as antibacterial agents is a farsighted and effective direction in the field of plant protection. The aim of the study was to develop a technology for production and control of Xanthomonas campestris phage biological product, taking into account previously defined technological parameters. The objects of the study were X. campestris pv. campestris Cl34-UlSAU bacteriophage isolated from cabbage samples with signs of vascular bacteriosis from the fields of Ulyanovsk region, Staromainsky district. As a production strain, we used X. campestris pv. campestris Xc2 bacterial strain. The authors carried out studies on selection of suitable conditions, taking into account previously defined parameters: a method for bacteriophage removal from a production culture of bacteria, suitable passage time for production of a phage preparation, appropriate balance of phage and bacterial culture for cultivation, suitable temperature for bacteriophage cultivation. The paper introduces a scheme for production and control of Xanthomonas campestris pv. campestris phage biological product, which consists of 4 stages: sample preparation of a production culture of bacteria for compliance with the properties of the declared strain, sample preparation of a production strain of Kl34-UlSAU bacteriophage for compliance with its activity after storage, production of a phage product with reference to production scaling, pouring, purity control, bacteriophage titer, its specificity and spectrum of lytic action, storage of a biological product.

The influence of bacteriophages on the manifestation of their pathogenic properties by Xanthomonas campestris bacteria

“…Currently, there is extensive data on the use of phage therapy in various fields, including in relation to plant pathogens [9][10][11]. The application of phage biopreparations in various methods allows controlling and analyzing the quantitative and qualitative composition of isolated bacteria, which, unlike classical bacteriological methods, takes much less time [12][13]. At the same time, it is important to understand that for the effective use of bacteriophages, it is necessary to understand how active they are and how quickly they are able to infect bacterial cells.…”