The variables related to the agro-industrial quality in sugarcane considered as main tools are being used by producers to choose the varieties to be planted. In general, cultivars that present a better industrial yield for the manufacture of sugar and alcohol have great importance promoting crop sustainability. In this research, the objective was to evaluate the industrial quality of four sugarcane varieties during plant-cane, first and second ratoon crops. The experimental design was a randomized block, with four treatments and five replications. The varieties studied were RB867515, RB92579, SP813250 and VAT90212. In first and second ratoon cycles, the industrial quality of the four varieties was evaluated by determining the fiber content, apparent sucrose (Pol), purity, soluble solids (Brix) and total recoverable sugars (TRS). The results showed that varieties RB867515, RB92579, SP813250 and VAT90212 were similar for agro-industrial quality. In first ratoon crop, only fiber was same between varieties. The RB867515 evidenced larger soluble solids and recoverable sugars. In second ratoon crop, the RB92579 evidenced larger apparent sucrose (Pol) and soluble solids (Brix) and total recoverable sugars (TRS).