Technological Aspects of β-Carotene Production

Ribeiro, Bernardo Dias; Barreto, Daniel Weingart; Coelho, Maria Alice Zarur

doi:10.1007/s11947-011-0545-3

Cited by 138 publications

(89 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The search for natural sources of β-carotene is of great interest, since only 2% of all commercial β-carotene is naturally produced worldwide (Dufossé et al, 2005;Ribeiro et al, 2011). Carotenoids attracted attention because a number of epidemiological studies have revealed that an (Stahl and Sies, 2003;Mayne, 1996).…”

Section: Total Carotenoidmentioning

confidence: 99%

“…The results indicate that this oil is one of the richest known sources of biological active β-carotene (781. 6 mg·kg −1 ), which imparts the characteristic orange-red color and also lends oxidative protection to the oil (Ribeiro et al, 2011;Benadé, 2013). In the literature, the total carotenoid content of Buriti oil fluctuates between 600 mg·kg −1 to 10,000 mg·kg −1…”

Section: Total Carotenoidmentioning

confidence: 99%

See 1 more Smart Citation

Amazonian Buriti oil: chemical characterization and antioxidant potential

et al. 2016

View full text Add to dashboard Cite

SUMMARY:Buriti oil is an example of an Amazonian palm oil of economic importance. The local population uses this oil for the prevention and treatment of different diseases; however, there are few studies in the literature that evaluate its properties. In this study, detailed chemical and antioxidant properties of Buriti oil were determined. The predominant fatty acid was oleic acid (65.6%) and the main triacylglycerol classes were tri-unsaturated (50.0%) and di-unsaturated-mono-saturated (39.3%) triacylglycerols. The positional distribution of the classes of fatty acids on the triacylglycerol backbone indicated a saturated and unsaturated fatty acid relationship similar in the three-triacylglycerol positions. All tocopherol isomers were present, with a total content of 2364.1 mg·kg −1 . α-tocopherol constitutes 48% of the total tocopherol content, followed by γ-tocopherol (45%). Total phenolic (107.0 mg gallic acid equivalent·g −1 oil) and β-carotene (781.6 mg·kg −1 ) were particularly high in this oil. The highest antioxidant activity against the free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) was obtained at an oil concentration of 50 mg·mL −1 (73.15%). The antioxidant activity evaluated by the Oxygen Radical Absorbance Capacity (ORAC) was 95.3 μmol Trolox equivalent·g −1 oil. These results serve to present Buriti oil as an Amazonian resource for cosmetic, food and pharmaceuticals purposes. KEYWORDS: Fatty acid; Minor compound; Radical scavenging activity; Regio-specific distribution; TriacylglycerolRESUMEN: Aceite de buriti de la Amazonia: Caracterización química y potencial antioxidante. El aceite de Buriti es un ejemplo de aceite de palma amazónica de gran importancia económica. La población local utiliza este aceite para la prevención y el tratamiento de diferentes enfermedades; sin embargo, hay pocos estudios científicos que evalúen sus propiedades. En este estudio, se determinaron las propiedades antioxidantes del aceite de Buriti. El ácido graso predominante fue el oleico (65,6 %) y las principales clases de triglicéridos fueron tri-insaturadas (50,0 %) y Di-insaturados-mono-saturada (39,3 %). La distribución posicional de las clases de ácidos grasos en el esqueleto de triacilglicerol indicó una relación de ácidos grasos saturados e insaturados similar en las tres posiciones del triacilglicerol. Todas las isoformas de tocoferol estaban presentes, con un contenido total de 2364.1 mg·kg ) fueron particularmente altos en este aceite. La mayor actividad antioxidante contra el radical 1,1-difenil-2-picrilhidrazil libre (DPPH) se obtuvo a una concentración de aceite de 50 mg·mL −1 (73,15 %). La actividad antioxidante evaluadas por la capacidad de absorción de radicales de oxígeno (ORAC) fue 95,3 mmol Trolox equivalente·g −1 de aceite. Estos resultados presentan al aceite de Buriti amazónico como buen recurso con fines cosmético, alimenticio y farmacéutico.

show abstract

Section: Total Carotenoidmentioning

confidence: 99%

Section: Total Carotenoidmentioning

confidence: 99%

Amazonian Buriti oil: chemical characterization and antioxidant potential

et al. 2016

View full text Add to dashboard Cite

show abstract

“…9 The accumulation capacity of β-carotene in Dunaliella is influenced by stress factors in culture, such as high light intensity and salt concentration, low temperatures and/or nitrate deficiency. 10 Studies reported in the literature aimed at increasing the production of carotenoids in Dunaliella showed that its concentration varies in a range between 4 and 38 µg mL -1 .…”

Section: Introductionmentioning

confidence: 99%

Generation of Volatile Compounds from Carotenoids ofDunaliella bardawilAlgae by Water Bath Heating and Microwave Irradiation

Tinoco¹,

Uekane²,

Tsukui³

et al. 2016

Journal of the Brazilian Chemical Society

View full text Add to dashboard Cite

The volatile compounds formed by thermal degradation of the carotenoids present in Dunaliella bardawil were investigated by microwave irradiation (MW) and water bath heating (WB) in different conditions of temperature and time using central composite design. Volatiles extraction by solid phase microextraction (SPME) was optimized and performed at 40 °C for 15 minutes, and those in greater amount were quantified by a validated method of gas chromatography coupled to a quadrupole mass spectrometer (GC-qMS). Employing WB, 10, 12 and 120 ng mL -1 of β-cyclocitral, α-ionone e β-ionone, respectively, were obtained between 60 and 87 °C for 30-75 min, while by MW, 5, 5, 50 ng mL -1 were obtained between 75-107 °C for 1.5-2.8 min. Considering the shorter time of MW, it can be concluded that if the time necessary to obtain the best yield by WB is employed in multiple MW cycles, an amount 10 times greater of those compounds would be obtained by MW than by WB. The results suggest a new biotechnology application for the carotenoids of the microalgae of the Dunaliella genus. Keywords: Dunaliella bardawil, β-cyclocitral, ionone, carotenoid, microwave, water bath IntroductionMicroalgae are photosynthetic microorganisms widely distributed in marine, freshwater and terrestrial ecosystems.1,2 They produce a great diversity of compounds with high commercial value, such as polyunsaturated fatty acids, carotenoids, phycobilins, polysaccharides, vitamins and sterols. [3][4][5][6][7] The production of microalgae supplies the cosmetic and food supplement industries, and boosts the development of natural food products, an emerging and promising field for industrial application.8 Microalgae have properties typical of higher plants (efficient oxygenic photosynthesis and simplicity of nutritional requirements), but also biotechnological attributes of microbial cells (rapid growth in culture medium and capacity to accumulate or secrete metabolites of interest), 4 supporting large scale applications.Among the microalgae used to obtain carotenoids, two species of the genus Dunaliella, D. bardawil and D. salina stand out for the production of β-carotene in their chloroplasts. 9 The accumulation capacity of β-carotene in Dunaliella is influenced by stress factors in culture, such as high light intensity and salt concentration, low temperatures and/or nitrate deficiency. 10 Studies reported in the literature aimed at increasing the production of carotenoids in Dunaliella showed that its concentration varies in a range between 4 and 38 µg mL -1 . 11-14Herrero et al. 15 evaluated the chemical composition and antimicrobial activity of the pressurized extract of D. salina. Norisoprenoids such as α-ionone, β-ionone and β-cyclocitral, besides neophytadiene and phytol were observed, as well as antimicrobial activity associated to volatiles. Previously, Larrouche et al. 16 showed that β-ionone inhibits the respiratory chain, preventing microbial oxygen consumption.Norisoprenoids can be formed from carotenoids by external agents such as temperature, light...

show abstract

“…Diversos estudos demonstram que a composição do meio empregado pode interferir na bioprodução desses pigmentos, além disso, outras condições externas do cultivo como temperatura, agitação, aeração e pH também levam a alterações como variedade e quantidade de carotenoides produzidos pelo micro-organismo (RIBEIRO, et al, 2011;SCHWARTZ, 2010).…”

Section: Introductionunclassified

INTERFERÊNCIA DO pH INICIAL E VOLUME DE MEIO NA PRODUÇÃO DE CAROTENOIDES POR Sporidiobolus pararoseus

Tavares¹,

Sá²,

Borba³

et al. 2015

Anais Do Congresso Brasileiro De Engenharia Química Em Iniciação Científica - Cobeq IC 2015

View full text Add to dashboard Cite

RESUMO -Fatores como composição do meio, temperatura, aeração e pH podem interferir na produção de microbiana de carotenoides. Em vista disso, o presente trabalho teve como objetivo avaliar o efeito do pH inicial (3 à 5) e a relação de volume de meio para volume de reator (3:10 à 7:10) no cultivo em frascos agitados para produção de carotenoides por S. pararoseus. Na faixa estudada, apenas a variável pH presentou um efeito significativo (p<0,05) sobre a resposta carotenoides totais. O valores máximo de carotenoides totais alcançados foram de 204,7 (ensaio 2) a 463,9 µg/L (ensaio 1) e para biomassa de 4,6 (ensaio 4) a 13,6 g/L (ensaio 1), sendo que os menores valores na produção de carotenoides e de biomassa ocorreram nos ensaios com pH 5,0 (nível +1). A produção em pH próximo a 3 levou a uma aumento e aproximadamente 56% na produção de carotenoides e 66% na biomassa foi produzida nesse pH do que em comparação ao pH 5. INTRODUÇÃOOs carotenoides estão amplamente distribuídos na natureza, apresentando papel de destaque na fotossíntese e fotoproteção de sistemas vegetais e como precursores de vitamina A nos seres humanos. Além de funções nos sistemas biológicos, apresentam também grande importância para a indústria farmacêutica na elaboração de cosméticos e como aditivo alimentar na indústria de alimentos (DAMODARAN, et al., 2010).Diversos estudos demonstram que a composição do meio empregado pode interferir na bioprodução desses pigmentos, além disso, outras condições externas do cultivo como temperatura, agitação, aeração e pH também levam a alterações como variedade e quantidade de carotenoides produzidos pelo micro-organismo (RIBEIRO, et al., 2011;SCHWARTZ, 2010).Em vista do apresentado o presente trabalho possui como objetivo avaliar a interferência do pH inicial e a relação Vmeio:Vreator na produção de carotenoides por Sporidiobolus pararoseus em frascos agitados MATERIAL E MÉTODOS

show abstract

Technological Aspects of β-Carotene Production

Cited by 138 publications

References 66 publications

Amazonian Buriti oil: chemical characterization and antioxidant potential

Amazonian Buriti oil: chemical characterization and antioxidant potential

Generation of Volatile Compounds from Carotenoids ofDunaliella bardawilAlgae by Water Bath Heating and Microwave Irradiation

INTERFERÊNCIA DO pH INICIAL E VOLUME DE MEIO NA PRODUÇÃO DE CAROTENOIDES POR Sporidiobolus pararoseus

Contact Info

Product

Resources

About