Techno-economic analysis and life-cycle greenhouse gas mitigation cost of five routes to bio-jet fuel blendstocks

Baral, Nawa Raj; Kavvada, Olga; Mendez-Perez, Daniel; Mukhopadhyay, Aindrila; Lee, Taek Soon; Simmons, Blake A.; Scown, Corinne D.

doi:10.1039/c8ee03266a

Cited by 120 publications

(122 citation statements)

References 63 publications

(111 reference statements)

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“…The remaining biomass sorghum after coproduct extraction is mixed with ILs and water at an IL-to-biomass ratio of 0.29 wt% and pretreated at 140°C for 3 h (46). Cholinium lysinate ([Ch][Lys]) is used as a representative IL due to its effectiveness in depolymerizing biomass and compatibility with enzyme and microbes (2). Following the pretreatment, H 2 SO 4 is added to adjust the pH to around 5 and the slurry is routed to the enzymatic hydrolysis unit.…”

Section: Methodsmentioning

confidence: 99%

“…To estimate the cost of recovering each of the five representative products (limonene, latex, PHB, cannabidiol, and artemisinin), we developed separate process simulation models in SuperPro Designer. In each case, chopped, dried sorghum biomass is delivered to the biorefinery at a cost of $95 per dry metric ton, which is based on the costs of cultivating, harvesting, and transporting sorghum (2). In the base case, this biomass is pretreated, undergoes simultaneous saccharification and fermentation, and the ethanol is recovered and sold as fuel.…”

Section: Simulated Cost Of Cellulosic Fuel Production and Coproduct Rmentioning

confidence: 99%

“…This lag is due to several factors, including the recent global economic recession in the late 2000s and the dramatic drop in oil prices in the mid-2010s, but one of the most important contributing factors is that cellulosic biofuels remain more expensive to produce than conventional fossil fuels when negative externalities are not fully accounted for. Improving biofuel yields and optimizing the deconstruction and conversion processes that produce them are important for reducing biofuel production costs and increasing market uptake, but some previous studies have shown that these approaches to process optimization alone are not necessarily sufficient to reach minimum selling prices competitive with current conventional fuel prices, even at near-theoretical yields from sugars (2). One strategy to overcome these challenges is the generation of valuable coproducts alongside biofuels by a cellulosic biorefinery (3).…”

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confidence: 99%

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Accumulation of high-value bioproducts in planta can improve the economics of advanced biofuels

Yang

Baral

Simmons

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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Coproduction of high-value bioproducts at biorefineries is a key factor in making biofuels more cost-competitive. One strategy for generating coproducts is to directly engineer bioenergy crops to accumulate bioproducts in planta that can be fractionated and recovered at biorefineries. Here, we develop quantitative insights into the relationship between bioproduct market value and target accumulation rates by investigating a set of industrially relevant compounds already extracted from plant sources with a wide range of market prices and applications, including <$10/kg (limonene, latex, and polyhydroxybutyrate [PHB]), $10 to $100/kg (cannabidiol), and >$100/kg (artemisinin). These compounds are used to identify a range of mass fraction thresholds required to achieve net economic benefits for biorefineries and the additional amounts needed to reach a target $2.50/gal biofuel selling price, using cellulosic ethanol production as a test case. Bioproduct market prices and recovery costs determine the accumulation threshold; we find that moderate- to high-value compounds (i.e., cannabidiol and artemisinin) offer net economic benefits at accumulation rates of just 0.01% dry weight (dwt) to 0.02 dwt%. Lower-value compounds, including limonene, latex, and PHB, require at least an order-of-magnitude greater accumulation to overcome additional extraction and recovery costs (0.3 to 1.2 dwt%). We also find that a diversified approach is critical. For example, global artemisinin demand could be met with fewer than 10 biorefineries, while global demand for latex is equivalent to nearly 180 facilities. Our results provide a roadmap for future plant metabolic engineering efforts aimed at increasing the value derived from bioenergy crops.

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Section: Methodsmentioning

confidence: 99%

Section: Simulated Cost Of Cellulosic Fuel Production and Coproduct Rmentioning

confidence: 99%

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confidence: 99%

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Accumulation of high-value bioproducts in planta can improve the economics of advanced biofuels

Yang

Baral

Simmons

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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“…Recent economic analyses highlight the necessity of lignin valorization to create a sustainable bioeconomy [17,18]. With its robust aromatic metabolism combined with novel methods of biomass deconstruction, P. putid a has great potential to convert lignocellulosic biomass to value-added products [19,20].…”

Section: Discussionmentioning

confidence: 99%

Host engineering for improved valerolactam production inPseudomonas putida

Thompson

et al. 2019

Preprint

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25Pseudomonas putida is a promising bacterial chassis for metabolic engineering given its 26 ability to metabolize a wide array of carbon sources, especially aromatic compounds derived 27 from lignin. However, this omnivorous metabolism can also be a hindrance when it can naturally 28 metabolize products produced from engineered pathways. Herein we show that P. putida is able 29 to use valerolactam as a sole carbon source, as well as degrade caprolactam. Lactams represent 30 important nylon precursors, and are produced in quantities exceeding one million tons per 31 year[1]. To better understand this metabolism we use a combination of Random Barcode 32 Transposon Sequencing (RB-TnSeq) and shotgun proteomics to identify the oplBA locus as the 33 likely responsible amide hydrolase that initiates valerolactam catabolism. Deletion of the oplBA 34 genes prevented P. putida from growing on valerolactam, prevented the degradation of 35 valerolactam in rich media, and dramatically reduced caprolactam degradation under the same 36 conditions. Deletion of oplBA, as well as pathways that compete for precursors L-lysine or 5-37 aminovalerate, increased the titer of valerolactam from undetectable after 48 hours of production 38 to ~90 mg/L. This work may serve as a template to rapidly eliminate undesirable metabolism in 39 non-model hosts in future metabolic engineering efforts. 40 1 INTRODUCTION 41 Pseudomonas putida has attracted great attention as a potential chassis organism for 42 metabolic engineering due in large part to its ability to metabolize a wide variety of carbon 43 sources, particularly aromatic compounds that can be derived from lignin [2,3]. To more fully 44 realize this vision, much effort has been put forth recently to better characterize the central 45 metabolism of P. putida with updated genome-scale models [4], C 13 flux experiments [5,6], and 46high-throughput fitness assays, which have all contributed to a more complete understanding of 47 48 fully understood. 49 One consequence of this omnivorous metabolism is that P. putida possesses the ability to 50 degrade or fully catabolize chemicals metabolic engineers seek to produce in the host. An 51 ongoing challenge for P. putida host engineering will be to rapidly identify catabolic pathways 52 of important target molecules and eliminate them from the genome. The recent report of a novel 53 pathway for levulinic acid catabolism in P. putida KT2440 underscores the catabolic flexibility 54 of the host, and an additional obstacle towards producing high product titer [8]. While 55 challenging, this is not surprising; as a genus, Pseudomonads are well known for their ability to 56 degrade a wide range of naturally occurring or xenobiotic chemicals [9,10]. 57 Caprolactam and valerolactam are both important commodity chemicals used in the 58 synthesis of nylon polymers, with global production of caprolactam reaching over four million 59 metric tons [1]. Multiple efforts have been made to produce these chemicals biologically, with 60the titers of valerolactam...

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“…Increasing concerns about the cost and environmental impact of petroleum-derived fuels (Baral et al, 2019) has motivated the development of microbial hosts for the production of fuels from renewable carbon sources (Cheon et al, 2016;Liao et al, 2016;Meadows et al, 2018;Rabinovitch-Deere et al, 2013). In particular, 3-methyl-3-buten-1-ol (isoprenol) is a promising alternative to gasoline due to its anti-knocking properties, comparable energy density, and comparable research octane number (Liu et al, 2014;Mack et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Optimization of the IPP-bypass mevalonate pathway and fed-batch fermentation for the production of isoprenol in Escherichia coli

Kang

Mendez-Perez

Goh

et al. 2019

Metabolic Engineering

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Techno-economic analysis and life-cycle greenhouse gas mitigation cost of five routes to bio-jet fuel blendstocks

Abstract: Decarbonizing the air transportation sector remains one of the most challenging hurdles to mitigating climate change.

Cited by 120 publications

References 63 publications

Accumulation of high-value bioproducts in planta can improve the economics of advanced biofuels

Accumulation of high-value bioproducts in planta can improve the economics of advanced biofuels

Host engineering for improved valerolactam production inPseudomonas putida

Optimization of the IPP-bypass mevalonate pathway and fed-batch fermentation for the production of isoprenol in Escherichia coli

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