Accumulation of high-value bioproducts in planta can improve the economics of advanced biofuels

Abstract

Coproduction of high-value bioproducts at biorefineries is a key factor in making biofuels more cost-competitive. One strategy for generating coproducts is to directly engineer bioenergy crops to accumulate bioproducts in planta that can be fractionated and recovered at biorefineries. Here, we develop quantitative insights into the relationship between bioproduct market value and target accumulation rates by investigating a set of industrially relevant compounds already extracted from plant sources with a wide range of market prices and applications, including <$10/kg (limonene, latex, and polyhydroxybutyrate [PHB]), $10 to $100/kg (cannabidiol), and >$100/kg (artemisinin). These compounds are used to identify a range of mass fraction thresholds required to achieve net economic benefits for biorefineries and the additional amounts needed to reach a target $2.50/gal biofuel selling price, using cellulosic ethanol production as a test case. Bioproduct market prices and recovery costs determine the accumulation threshold; we find that moderate- to high-value compounds (i.e., cannabidiol and artemisinin) offer net economic benefits at accumulation rates of just 0.01% dry weight (dwt) to 0.02 dwt%. Lower-value compounds, including limonene, latex, and PHB, require at least an order-of-magnitude greater accumulation to overcome additional extraction and recovery costs (0.3 to 1.2 dwt%). We also find that a diversified approach is critical. For example, global artemisinin demand could be met with fewer than 10 biorefineries, while global demand for latex is equivalent to nearly 180 facilities. Our results provide a roadmap for future plant metabolic engineering efforts aimed at increasing the value derived from bioenergy crops.

Strategies for the production of biochemicals in bioenergy crops

“…Recently, the overproduction of limonene has been successfully demonstrated in oilseed crop (Camelina sativa) by overexpressing LMS under the control of Arabidopsis promoters [286]. A recent study indicated that reaching limonene titers of 2.2% DW in bioenergy crops and extracting this coproduct from biomass at 70% efficiency could positively impact the economics of advanced biofuels [8].…”

“…In addition, CRISPR/Cas9 genome editing has been demonstrated in rubber dandelion, which provides an avenue to accelerate the investigation of rubber biosynthesis [315]. Based on a techno-economic analysis, the accumulation of latex in bioenergy crops at titers of 2.2% DW could significantly improve the economics of second-generation biofuels [8].…”

“…Similarly, promising strategies have been developed for introducing organelles that allow bioproduct sequestration and accumulation in engineered plant tissues [319]. In several cases, extraction and purification of biochemicals from plant biomass represent other important challenges to overcome for rendering biorefineries economically attractive [8]. In addition to the work conducted by plant metabolic engineers to increase titers of specific chemicals in crops, which in some instances has resulted in remarkable increases by more than two orders of magnitude after several years of research [320], an emphasis should also be given to the development of isolation and purification processes to render plant-derived chemicals economically competitive compared to their petroleum-derived equivalents.…”

“…In fact, accumulation of bioproducts or metabolic intermediates in crops may benefit specific biorefinery configurations in which hydrolysates are fractionated to derive maximum value from each component [2]. For example, it has been recently documented from techno-economic analyses that co-production of high-value chemicals in bioenergy crops has the potential to ameliorate the economics of advanced biofuels obtained from lignocellulosic biomass [8].…”

Redesigning plant cell walls for the biomass-based bioeconomy

“…In a circular economy, monomers should be recovered and re-usable for subsequent polymerization without adversely affecting material quality. However, the combination of low market value and poor accumulation rates of plantbased plastics might represent an insurmountable challenge in the short run, favoring the production of higher-value coproducts from biomass (117). In that regard, chemical modifications of lignins into high-value products, such as aromas, flavors and essential oils, represents a potential economic incentive for biorefineries (78,118).…”