Techniques using ImageJ for histomorphometric studies

Curvo, Lucimar Rodrigues Vieira; Ferreira, Milena Wolff; Costa, Celso Soares; Barbosa, Guilherme Ribeiro Capibaribe; Uhry, Sandra Adriana; Silveira, Ulisses Simon da; Silva, Alanderson Rodrigues da; Andrade, Gisele Braziliano de

doi:10.33448/rsd-v9i11.9586

Cited by 2 publications

(2 citation statements)

References 36 publications

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“…(2023) and Curvo et al. (2020), we used histological photographs and Fiji‐ImageJ software (Schindelin et al., 2012) to determine the number of goblet cells, thickness of the muscle layer, and the perimeter and area of the lumen, as a proxy for the absorption surface. We analyzed these variables only for the esophagus and intestine segments, as the degree of stomach fullness (i.e., full vs. empty) could cause measurement bias.…”

Section: Methodsmentioning

confidence: 99%

“…For a quantitative examination of the digestive tract traits, we took three histological photographs of each segment from five individuals per species. Following the methodology outlined by Bellinate et al (2023) and Curvo et al (2020), we used histological photographs and Fiji-ImageJ software (Schindelin et al, 2012) to determine the number of goblet cells, thickness of the muscle layer, and the perimeter and area of the lumen, as a proxy for the absorption surface. We analyzed these variables only for the esophagus and intestine segments, as the degree of stomach fullness (i.e., full vs. empty) could cause measurement bias.…”

Section: Histology and Histochemistrymentioning

confidence: 99%

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Linking anatomical and histological traits of the digestive tract to resource consumption and assimilation of omnivorous tetra fishes

Neves,

Amorim,

Delariva

et al. 2024

Ecology and Evolution

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This study explores the interplay between digestive tract traits, food intake, and assimilation in omnivorous tetra fishes (Psalidodon bifasciatus, P. aff. gymnodontus, and Bryconamericus ikaa) from the Iguaçu River basin, an ecologically significant region known for high endemism. We hypothesize that variations in digestive tracts across species would be associated with differences in diet, isotopic composition in fish tissues, and overall diet assimilation. To test this, we employed stereoscopic and light microscopy to characterize the gross anatomy, histomorphology, and histochemistry of fish digestive tracts. Additionally, we used stomach content and stable isotope analyses to trace fish diets. While these tetra fishes shared histological structures, disparities were noted in anatomical digestive traits and diet preferences. The smallest species, B. ikaa, with a shorter intestine, had fewer pyloric caeca and primarily consumed animal‐based diets. Conversely, P. bifasciatus and P. aff. gymnodontus, with longer intestines, displayed numerous pyloric caeca and consumed a balanced mix of animal and plant items. Despite anatomical and dietary differences, all three species predominantly assimilated animal‐origin food. The tetra fishes had histological variations among digestive tract segments, with the esophagus having the thickest muscular layer, gradually thinning towards the posterior intestine. The final portion of the intestine exhibited a significant expansion in the lumen perimeter, while the esophagus had the smallest lumen area. Goblet cells were most concentrated in the posterior intestine for all species. The gross anatomy of these tetra fishes aligns with their omnivorous habit, while diet assimilation was dominated by animal‐origin food. These findings provide crucial insights into the structural and tissue characteristics of their digestive systems, laying the groundwork for deeper exploration into the physiological aspects of their digestive tracts and enhancing our understanding of their feeding strategies.

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Section: Methodsmentioning

confidence: 99%

Section: Histology and Histochemistrymentioning

confidence: 99%

Linking anatomical and histological traits of the digestive tract to resource consumption and assimilation of omnivorous tetra fishes

Neves,

Amorim,

Delariva

et al. 2024

Ecology and Evolution

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Tomographic criteria for assessing the structural state of kidney stones

Kolupayev,

Lisovyi,

Demchenko

et al. 2021

MTT

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Urolithiasis is one of the most common urological diseases. The modern approach to the treatment of this pathology involves the use of a wide range of minimally invasive surgical interventions, the main stage of which is the destruction of the stone with subsequent removal of its fragments. Preoperative diagnosis of physicochemical parameters of kidney stones is of great practical importance in the aspect of choosing a treatment method, especially in the case of planning extracorporeal shock wave lithotripsy. The article examines the peculiarities of tomographic images of kidney stones with various structural features. The study consisted in the study of the microstructure of stones removed as a result of minimally invasive surgical interventions (extracorporeal shock wave, percutaneous and ureteroscopic lithotripsy) in 63 patients with urolithiasis, by the method of crystal-optical analysis on a polarizing microscope, with subsequent digital analysis of their tomographic images, according to using the ImageJ software package, with determination of the average pixel intensity (PI) in the gray scale range of 0–250. During the crystal-optical analysis, it was established that regardless of the mineral composition of the stone, the inorganic components that make up its composition can be in an amorphous or crystalline state. The structural types of kidney stones were determined based on the determination of the volume fraction of the crystalline phase (VFCP) in the structure of the urolith. Structural type A – VFCF <50%vol, structural type B – VFCF >50%vol, structural type C – VFCF = 100%vol. When analyzing tomographic images, it was found that kidney stones belonging to different structural types were characterized by different average pixel intensity (PI). A positive correlation between VFCP and PI was established, as well as reliable differences in the PI indicator between groups of stones of the first and third degree of crystallinity, which allows considering this indicator as a tomographic criterion of the degree of crystallinity of a kidney stone, the determination of which is expedient at the stage of choosing a lithotripsy method. Keywords: urollith, structure, crystallinity, tomographic image.

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Techniques using ImageJ for histomorphometric studies

Cited by 2 publications

References 36 publications

Linking anatomical and histological traits of the digestive tract to resource consumption and assimilation of omnivorous tetra fishes

Linking anatomical and histological traits of the digestive tract to resource consumption and assimilation of omnivorous tetra fishes

Tomographic criteria for assessing the structural state of kidney stones

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