Techniques of Experimentation

Talcott, Michael; Akers, Walter J.; Marini, Robert P.

doi:10.1016/b978-0-12-409527-4.00025-0

Cited by 3 publications

(3 citation statements)

References 356 publications

(287 reference statements)

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“…15,16 The IP route of drug administration was chosen as the most accurate and reliable way to control the dose of compound administered to the animal and is recognized as a standard practice in in vivo studies. 77 Model of Depressive-like State Induced by 21-Day Variable-Frequency Ultrasound Exposure. This protocol was used to induce a depressive-like state in mice and rats, as previously proposed.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…In the control groups, animals received a sterile 0.9% saline solution (containing 0.1% Tween 20). The dose was selected according to the literature data. , The IP route of drug administration was chosen as the most accurate and reliable way to control the dose of compound administered to the animal and is recognized as a standard practice in in vivo studies …”

Section: Materials and Methodsmentioning

confidence: 99%

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Antidepressant-like Effect of the Eburnamine-Type Molecule Vindeburnol in Rat and Mouse Models of Ultrasound-Induced Depression

Zubkov,

Riabova,

Zorkina

et al. 2024

ACS Chem. Neurosci.

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Vindeburnol (VIND, RU24722, BC19), a synthetic molecule derived from the eburnamine-vincamine alkaloid group, has many neuropsychopharmacological effects, but its antidepressant-like effects are poorly understood and have only been described in a few patents. To reliably estimate this effect, vindeburnol was studied in a model of long-term variable-frequency ultrasound (US) exposure at 20–45 kHz in male Wistar rats and BALB/c mice. Vindeburnol was administered chronically for 21 days against a background of simultaneous ultrasound exposure at a dose of 20 mg/kg intraperitoneally (IP). Using four behavioral tests, the sucrose preference test (SPT), the social interaction test (SIT), the open field test (OFT), and the forced swimming test (FST), we found that the treatment with the compound diminished depression-like symptoms in mice and rats. The compound restored the ultrasound-related reduced sucrose consumption to control levels and increased social interaction time in mice and rats compared with those in ultrasound-exposed animals. Vindeburnol showed contraversive results of horizontal and vertical activity in both species and generally did not increase locomotor activity. At the same time, the compound showed a specific effect in the FST, significantly reducing the immobility time. Moreover, we found an increase in norepinephrine, dopamine, and its metabolite levels in the brainstem, as well as an increase in dopamine, 3-methoxytyramine, and 3,4-dihydroxyphenylacetic acid levels in the striatum. We also observed a statistically significant increase in tyrosine hydroxylase (TH) levels in the region containing the locus coeruleus (LC). We suggest that using its distinct chemical structure and pharmacological activity as a starting point could boost antidepressant drug discovery.

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Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: Materials and Methodsmentioning

confidence: 99%

Antidepressant-like Effect of the Eburnamine-Type Molecule Vindeburnol in Rat and Mouse Models of Ultrasound-Induced Depression

Zubkov,

Riabova,

Zorkina

et al. 2024

ACS Chem. Neurosci.

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“…Telemetry combines the use of different sensors and wireless communications to perform physical and/or chemical measurements remotely. Applied to the study of animals, it allows for the acquisition of animal life data through a device placed on the animal that sends signals to a receptor [ 1 ]. In this way, different issues related to the individual and their environment can be monitored in a much less invasive way, without having to come into direct contact with them, except for the placement of the device.…”

Section: Introductionmentioning

confidence: 99%

Modular E-Collar for Animal Telemetry: An Animal-Centered Design Proposal

Siguín

Blanco

Rossano

et al. 2021

Sensors

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Animal telemetry is a subject of great potential and scientific interest, but it shows design-dependent problems related to price, flexibility and customization, autonomy, integration of elements, and structural design. The objective of this paper is to provide solutions, from the application of design, to cover the niches that we discovered by reviewing the scientific literature and studying the market. The design process followed to achieve the objective involved a development based on methodologies and basic design approaches focused on the human experience and also that of the animal. We present a modular collar that distributes electronic components in several compartments, connected, and powered by batteries that are wirelessly recharged. Its manufacture is based on 3D printing, something that facilitates immediacy in adaptation and economic affordability. The modularity presented by the proposal allows for adapting the size of the modules to the components they house as well as selecting which specific modules are needed in a project. The homogeneous weight distribution is transferred to the comfort of the animal and allows for a better integration of the elements of the collar. This device substantially improves the current offer of telemetry devices for farming animals, thanks to an animal-centered design process.

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