Techniques for quantitative analyses of calcareous marine phytoplankton

Bollmann, Jörg; Cortés, Mara Y.; Haidar, Ali T.; Brabec, Bernhard; Close, Anne; Hofmann, Robert; Palma, S.; Tupas, Luis M.; Thierstein, Hans R.

doi:10.1016/s0377-8398(01)00040-8

Cited by 79 publications

(55 citation statements)

References 3 publications

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“…Additionally, samples can be prepared for light microscopy either by filtration or by using the Utermöhl sedimentation method (Utermöhl, 1958). Reid (1980) and Bollmann et al (2002) both concluded that inverted light microscopy is unreliable for determining cell densities of small coccolithophores.…”

Section: Discussionmentioning

confidence: 99%

“…Cell counts from SEM can additionally be unreliable at high cell densities, where shedded coccoliths can lead to difficulties in distinguishing individual coccospheres (A. Poulton, personal observation). The synthesis of datasets obtained from these different methods would be greatly improved by further comparative studies similar to those carried out by Bollmann et al (2002), as it is currently unclear to what extent small and rare species are being overlooked in different ocean regions as a result of these methodological differences.…”

Section: Discussionmentioning

confidence: 99%

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Global marine plankton functional type biomass distributions: coccolithophores

O'Brien¹,

Peloquin²,

Vogt³

et al. 2013

Earth Syst. Sci. Data

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Abstract. Coccolithophores are calcifying marine phytoplankton of the class Prymnesiophyceae. They are considered to play an import role in the global carbon cycle through the production and export of organic carbon and calcite. We have compiled observations of global coccolithophore abundance from several existing databases as well as individual contributions of published and unpublished datasets. We make conservative estimates of carbon biomass using standardised conversion methods and provide estimates of uncertainty associated with these values. The quality-controlled database contains 57 321 individual observations at various taxonomic levels. This corresponds to 11 503 observations of total coccolithophore abundance and biomass. • S, with declines towards both the equator and the poles. Biomass estimates between the equator and 40•

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Global marine plankton functional type biomass distributions: coccolithophores

O'Brien¹,

Peloquin²,

Vogt³

et al. 2013

Earth Syst. Sci. Data

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“…The samples were investigated using a Motic Polarizing Microscope (PM, BA300) under 1000 × magnification with more than 300 coccoliths or 100 coccospheres being identified and counted per filter according to Heimdal (1997), Bollmann et al (2002), Yang et al (2003), Jordan et al (2004) and Frada et al (2010). Coccolith/coccosphere abundance was calculated following the method described in Sun et al (2011) as the following equation:…”

Section: Coccolith and Coccosphere Data Analysis And Statistical Methodsmentioning

confidence: 99%

Summer and winter living coccolithophores in the Yellow Sea and the East China Sea

Sun

Feng

et al. 2014

Biogeosciences

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Abstract. This paper describes the distribution of living coccolithophores (LCs) in the Yellow Sea and the East China Sea in summer and winter, and its relationship with environmental factors by canonical correspondence analysis (CCA). We carried out a series of investigations on LCs distribution in the Yellow Sea and the East China Sea in July and December 2011. 210 samples from different depths were collected from 44 stations in summer and 217 samples were collected from 45 stations in winter. Totally 20 taxa belonging to coccolithophyceae were identified using a polarized microscope at the 1000 × magnification. The dominant species of the two seasons were Gephyrocapsa oceanica, Emiliania huxleyi, Helicosphaera carteri, and Algirosphaera robusta. In summer the abundance of coccolithophore cells and coccoliths ranged 0-176.40 cells mL −1 , and 0-2144.98 coccoliths mL −1 , with the average values of 8.45 cells mL −1 , and 265.42 coccoliths mL −1 respectively. And in winter the abundance of cells and coccoliths ranged 0-71.66 cells mL −1 , and 0-4698.99 coccoliths mL −1 , with the average values of 13.91 cells mL −1 and 872.56 coccoliths mL −1 , respectively. In summer, the LCs in surface layer were mainly observed on the coastal belt and southern part of the survey area. In winter, the LCs in surface layer had high value in the continental shelf area of section P. The comparison among section A, section F, section P and section E indicated lower species diversity and less abundance in the Yellow Sea than those in the East China Sea in both seasons. Temperature and the nitrate concentration may be the major environmental factors controlling the distribution and species composition of LCs in the studying area based on CCA.

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“…The qualitative microscopic screening was performed to verify the presence of the dominant phytoplankton groups in conjunction with specific pigment biomarkers (Havskum et al, 2004;Irigoien et al, 2004). Sea surface water samples (1.0 l) for scanning electron microscopy (SEM) were filtered through polycarbonate membrane filters (0.8 lm pore size, Ø 47 mm, Millipore), dried onboard for 12 h at 50°C and stored dry before being mounted onto microscope slides and coated with gold (Bollmann et al, 2002). Coccolithophores were identified by scanning electron microscopy (SEM) using a Jeol JSM 5600 LV at a minimum magnification of 600 times.…”

Section: Microscopic Phytoplankton Identificationmentioning

confidence: 99%

Phytoplankton community dynamics during late spring coccolithophore blooms at the continental margin of the Celtic Sea (North East Atlantic, 2006–2008)

Oostende

Harlay

Vanelslander

et al. 2012

Progress in Oceanography

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a b s t r a c tWe determined the spatial and temporal dynamics of major phytoplankton groups in relation to biogeochemical and physical variables during the late spring coccolithophore blooms (May-June) along and across the continental margin in the Celtic Sea (2006Sea ( -2008. Photosynthetic biomass (chl a) of the dominant plankton groups was determined by CHEMTAX analysis of chromatographic (HPLC) pigment signatures.Phytoplankton standing stock biomass varied substantially between and during the campaigns (areal chl a [mg chl a m À2 ] in June 2006: 63.8 ± 26.5, May 2007: 27.9 ± 8.4, and May 2008: 41.3 ± 21.8), reflecting the different prevailing conditions of weather, irradiance, and sea surface temperature between the campaigns. Coccolithophores, represented mainly by Emiliania huxleyi, and diatoms were the dominant phytoplankton groups, with a maximal contribution of, respectively, 72% and 89% of the total chl a. Prasinophytes, dinoflagellates, and chrysophytes often co-occurred during coccolithophorid blooms, while diatoms dominated the phytoplankton biomass independently of the biomass of other groups. The location of the stations on the shelf or on the slope side of the continental margin did not influence the biomass and the composition of the phytoplankton community despite significantly stronger water column stratification and lower nutrient concentrations on the shelf. The alternation between diatom and coccolithophorid blooms of similar biomass, following the mostly diatom-dominated main spring bloom, was partly driven by changes in nutrient stoichiometry (N:P and dSi:N). High concentrations of transparent exopolymer particles (TEP) were associated with stratified, coccolithophore-rich water masses, which probably originated from the slope of the continental margin and warmed during advection onto the shelf. Although we did not determine the proportion of export production attributed to phytoplankton groups, the abundance of coccolithophores, together with TEP and coccoliths may affect the carbon export efficiency through increased sinking rates of particles formed by aggregation of TEP and coccoliths.

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Techniques for quantitative analyses of calcareous marine phytoplankton

Cited by 79 publications

References 3 publications

Global marine plankton functional type biomass distributions: coccolithophores

Global marine plankton functional type biomass distributions: coccolithophores

Summer and winter living coccolithophores in the Yellow Sea and the East China Sea

Phytoplankton community dynamics during late spring coccolithophore blooms at the continental margin of the Celtic Sea (North East Atlantic, 2006–2008)

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