Techniques for pharmacological and toxicological studies with isolated hepatocyte suspensions

Berry, Michael N.; Halls, Heather; Grivell, Marlene B.

doi:10.1016/0024-3205(92)90212-8

Cited by 85 publications

(25 citation statements)

References 172 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CCl 4 was metabolized to CCl 3 -and Cl-by microsomal cytochrome P450 system in liver cell, and produce trichloromethyl peroxy radical (·OOCCl 3 ), ·OOCCl 3 attacked unsaturated fatty acids of phospholipids in the cell membrane, leading to lipid peroxidation in the liver cells and generated amount of free radicals ( Halliwell and Gutteridge, 1997). Hepatocellular damage could lead to glutamic-pyruvic transaminase (GPT) and glutamic oxalacetic transaminase (GOT) to penetrate into the blood, which caused the raise of GOT and GPT in the serum (Romero et al, 1998;Berry et al, 1992). At the same time, lipid peroxidation could produce some free radicals including malondialdehyde (MDA).…”

Section: Discussionmentioning

confidence: 99%

Antioxidantactivities In Vitro And Hepatoprotective Effects Of Nelumbo Nucifera Leaves In Vivo

Yuan

Gu²,

Yin³

2014

Afr. J. Trad. Compl. Alt. Med.

View full text Add to dashboard Cite

Background: Herbal medicines played a major role in the treatment of hepatic disorders, and a number of medicinal plants and their compounds were widely used for the treatment of these disorders, and oxidant stress injury was one of the mechanism of liver injury. Materials and Methods:Antioxidant activity of Nelumbo nucifera leaves (NU) extracts was assayed by the methods of scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2´-azino-bis (3-ethylbenzo-thiazoline-6-sulfonicacid) (ABTS) radical and ferric reducing antioxidant power (FRAP) in vitro. By intraperitoneal injection carbon tetrachloride (CCl 4 ) to establish acute liver injury model in mice, the levels of Glutamic-pyruvic transaminase (GPT), glutamic-oxaloacetic transaminase (GOT), superoxide dismutase (SOD) and the content of and maleicdialdehyde (MDA) were detected to evaluate hepatoprotective effect of NU using corresponding test kit.Results: EtOAC (NUEA) and n-BuOH extracts (NUBU) of N. nucifera leaves had good scavenging DPPH and ABTS radical activity and ferric reducing antioxidant power in vitro. DPPH radical scavenging activity and ferric reducing antioxidant power of NUEA (IC 50 = 6.68±0.29 µg/mL, RACT 50 =1749.82±67.03 µmol/g) and NUBU (IC 50 = 4.61±0.01 µg/mL, RACT 50 =1995.27±135.71 µmol/g ) were higher than that of BHT (IC 50 =8.76±0.20 µg/mL, RACT 50 =1581.68±97.41 µmol/g) and Dangfeiliganning (IC 50 =28.06±0.17 µg/mL, RACT 50 =1028.55±3.28 µmol/g).ABTS radical scavenging activity of NUEA (IC 50 = 5.32±0.12 µg/mL) and NUBU (IC 50 = 8.16±0.27 µg/mL) were higher than that of Dangfeiliganning (IC 50 = 9.76±0.16 µg/mL). Thus, hepatoprotective effect of NUEA and NUBU was evaluated on CCl 4 -induced acute liver injury mice. The results showed that the levels of GOT and GPT in each treatment group significantly decreased (p<0.001 and p<0.01, p<0.05, respectively) except for the group of NUEA (130.8 mg/kg) (p>0.05). The contents of malondialdehyde (MDA) in liver in groups of NUEA (523 mg/kg), NUBU (840.5 and 420.5 mg/kg, repectively) had significant decrease (p<0.001 and p<0.05, respectively), and the level of SOD in liver for each treatment group could significantly decrease (p<0.001, p<0.05, respectively).Conclusion: NUEA and NUBU had significantly hepatoprotective effect for Calcium tetrachloride CCl 4 -induced liver injury, which might be attributable to its antioxidant activity.

show abstract

Section: Discussionmentioning

confidence: 99%

Antioxidantactivities In Vitro And Hepatoprotective Effects Of Nelumbo Nucifera Leaves In Vivo

Yuan

Gu²,

Yin³

2014

Afr. J. Trad. Compl. Alt. Med.

View full text Add to dashboard Cite

show abstract

“…Cells were incubated with compounds at increasing concentrations during 24 h and toxicity was evaluated using two assays in parallel, namely the release of lactate dehydrogenase (LDH), a cytoplasmic enzyme, and trypan blue exclusion assay [39]. This method represents a valid alternative to the MTT assay previously performed [11,40].…”

Section: Toxicity For Eukaryotic Cellsmentioning

confidence: 99%

2-Aminobenzothiazole derivatives: Search for new antifungal agents

Catalano

Carocci

Defrenza

et al. 2013

European Journal of Medicinal Chemistry

View full text Add to dashboard Cite

a b s t r a c tA new series of 6-substituted 2-aminobenzothiazole derivatives were synthesized and screened in vitro as potential antimicrobials. Almost all the compounds showed antifungal activity. In particular, compounds 1n,o, designed on the basis of molecular modeling studies, were the best of the series, showing MIC values of 4e8 mg/mL against Candida albicans, Candida parapsilosis and Candida tropicalis. None of the two compounds did show any cytotoxicity effect on human THP-1 cells.

show abstract

“…Plasma was prepared from EDTA-treated blood collected from the retro-orbital plexus. Hepatocytes were prepared from anesthetized mice by in situ liver perfusion and Percoll gradient separation, as described previously (41). Thioglycolate-elicited macrophages and granulocytes were collected by peritoneal lavage 24 or 48 h after i.p.…”

Section: Harvesting Of Normal and Inflammatory-challenged Tissuesmentioning

confidence: 99%

Differences in Wound Healing in Mice with Deficiency of IL-6 versus IL-6 Receptor

McFarland-Mancini

Funk

Paluch

et al. 2010

The Journal of Immunology

218

177

View full text Add to dashboard Cite

IL-6 modulates immune responses and is essential for timely wound healing. As the functions mediated by IL-6 require binding to its specific receptor, IL-6Rα, it was expected that mice lacking IL-6Rα would have the same phenotype as IL-6–deficient mice. However, although IL-6Rα–deficient mice share many of the inflammatory deficits seen in IL-6–deficient mice, they do not display the delay in wound healing. Surprisingly, mice with a combined deficit of IL-6 and IL-6Rα, or IL-6–deficient mice treated with an IL-6Rα–blocking Ab, showed improved wound healing relative to mice with IL-6 deficiency, indicating that the absence of the receptor contributed to the restoration of timely wound healing, rather than promiscuity of IL-6 with an alternate receptor. Wounds in mice lacking IL-6 showed delays in macrophage infiltration, fibrin clearance, and wound contraction that were not seen in mice lacking IL-6Rα alone and were greatly reduced in mice with a combined deficit of IL-6 and IL-6Rα. MAPK activation-loop phosphorylation was elevated in wounds of IL-6Rα–deficient mice, and treatment of wounds in these mice with the MEK inhibitor U0126 resulted in a delay in wound healing suggesting that aberrant ERK activation may contribute to improved healing. These findings underscore a deeper complexity for IL-6Rα function in inflammation than has been recognized previously.

show abstract

Techniques for pharmacological and toxicological studies with isolated hepatocyte suspensions

Cited by 85 publications

References 172 publications

Antioxidantactivities <i>In Vitro</i> And Hepatoprotective Effects Of <i>Nelumbo Nucifera</i> Leaves <i>In Vivo</i>

Antioxidantactivities <i>In Vitro</i> And Hepatoprotective Effects Of <i>Nelumbo Nucifera</i> Leaves <i>In Vivo</i>

2-Aminobenzothiazole derivatives: Search for new antifungal agents

Differences in Wound Healing in Mice with Deficiency of IL-6 versus IL-6 Receptor

Contact Info

Product

Resources

About