Technique for Staining Leafhopper (Homoptera: Cicadellidae) Salivary Sheaths and Eggs Within Unsectioned Plant Tissue

Backus, Elaine A.; Hunter, Wayne B.; Arne, C. N.

doi:10.1093/jee/81.6.1819

Cited by 62 publications

(38 citation statements)

References 3 publications

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“…Full pink, also known as the popcorn stage, is the stage when all the petals are visible and is the stage before bloom. Eggs were stained using a method adapted by Teulon et al (1993) from Backus et al (1988). Buds were sectioned, stained, cleared, and then examined under 250ϫ power using a compound microscope.…”

Section: Methodsmentioning

confidence: 99%

Damage to Nectarines by the Western Flower Thrips (Thysanoptera: Thripidae) in the Interior of British Columbia, Canada

Pearsall¹

2000

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The phenology of damage by the western flower thrips, Frankliniella occidentalis (Pergande), on nectarines was investigated using sticky cards and direct sampling of buds between 1993 and 1995 in the interior of British Columbia, the most susceptible period for damage by western flower thrips to nectarines. The life stage responsible for damage and variation in susceptibility to damage of 11 different nectarine varieties were determined. To evaluate the predictive ability of 2 sampling methods, thrips were counted from both buds and sticky cards before petal fall and correlated to damage levels at husk drop. Damage to nectarines was caused almost entirely by larval feeding at petal fall. No predictive relationships between adult or larval densities of western flower thrips and subsequent damage to fruit were apparent. Varieties did not differ in terms of larval densities at petal fall or the subsequent damage to fruit. Female western flower thrips oviposit in nectarine buds from dormant through bloom stages primarily in sepal tissues in the early buds, and in filaments and petals as these become available.

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Section: Methodsmentioning

confidence: 99%

Damage to Nectarines by the Western Flower Thrips (Thysanoptera: Thripidae) in the Interior of British Columbia, Canada

Pearsall¹

2000

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“…Th e feeding sites of adults and immature instars were observed on 40 leaves of each host. Th ey were sectioned in small blocks and the Backus et al (1988) staining technique was used. Th e sections were examined using light microscopy.…”

Section: Feeding Behaviormentioning

confidence: 99%

Feeding behavior of the vectorDelphacodes kuscheli(Hemiptera: Fulgoromorpha: Delphacidae) on maize and oat

Brentassi

Lenicov

2007

Annales de la Société entomologique de France (N.S.)

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Abstract. The planthopper Delphacodes kuscheli Fennah 1955 is an important pest affecting maize in Argentina. It transmits, in a persistent way, Mal de Río Cuarto virus (MRCV) (Reoviridae, Fijivirus) which has been reported to cause a severe loss in maize crops (Zea mays L.). The present study reports on the feeding behavior of the vector (adults and immature instars) on oat (preferential host) and maize (non-preferred host). Feeding sites and salivary sheaths were detected through histological sections of leaves. The location of salivary sheath terminations and the honeydew excretion analysis revealed that D. kuscheli feeds on phloem sap in both hosts. On maize, the high number of sheaths ending in the mesophyll and their particular disposition (parallel to the leaf surface and across the vascular bundles) strongly suggest the non-preference of the vector to feed on this species. On both hosts, immature instars showed similar feeding behavior to adults indicating the capacity to transmit the virus along the life period. Résumé. Comportement alimentaire du vecteur

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“…Southern chinch bug-infested axillary shoots were excised from the plants and placed in McBrideÕs staining solution. The staining solution was prepared using 0.2% (wt:vol) acid fuchsin in a 1:1 mixture of 95% (vol:vol) ethanol and glacial acetic acid (Sigma-Aldrich, St. Louis, MO), according to the methods of Backus et al (1988). The plant tissues were stained for 24 h at 24 Ϯ 2ЊC and then placed in the clearing solution and autoclaved for 15 min at 120ЊC and 15 psi.…”

Section: Methodsmentioning

confidence: 99%

Role of Leaf Sheath Lignification and Anatomy in Resistance Against Southern Chinch Bug (Hemiptera: Blissidae) in St. Augustinegrass

Rangasamy¹,

Rathinasabapathi²,

McAuslane³

et al. 2009

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Southern chinch bug, Blissus insularis Barber (Hemiptera: Blissidae), is the most serious insect pest of St. Augustinegrass Stenotaphrum secundatum (Walter) Kuntze, a common lawngrass grown in southeastern U.S. states. Host plant resistance to southern chinch bug has been identified in the polyploid St. Augustinegrass 'FX-10' and the diploid 'Captiva'. The objective of this research was to identify possible physical mechanism(s) explaining chinch bug resistance in these cultivars. We studied the distribution of chinch bug salivary sheaths in the preferred tissue for feeding (the axillary shoot) of the two resistant cultivars and two susceptible cultivars, paired for ploidy ('Floratam', polyploid, and Palmetto, diploid). We also investigated the potential role of axillary shoot lignification and anatomy in chinch bug resistance. Salivary sheaths were more abundant on the outermost leaf sheath of axillary shoots of resistant cultivars compared with susceptible cultivars. In contrast, fewer salivary sheaths reached the innermost meristematic tissue in the axillary shoots of resistant St. Augustinegrass cultivars than in the two susceptible cultivars. The polyploid cultivars FX-10 and Floratam had higher total lignin in axillary shoots compared with the diploid cultivars Captiva and Palmetto. However, total lignin content was not correlated with resistance to southern chinch bug. Light microscopic studies found no differences in epidermal layer thickness among resistant and susceptible St. Augustinegrass cultivars. However, transmission electron microscopic studies revealed that the cell walls of the sclerenchyma cells around the vascular bundle of southern chinch bug-resistant FX-10 and Captiva were significantly thicker than the cell walls in susceptible Floratam and Palmetto. Our research suggests that the thick-walled sclerenchyma cells around the vascular bundle play a role in southern chinch bug resistance in St. Augustinegrass, possibly by reducing stylet penetration to the vascular tissue.

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Technique for Staining Leafhopper (Homoptera: Cicadellidae) Salivary Sheaths and Eggs Within Unsectioned Plant Tissue

Cited by 62 publications

References 3 publications

Damage to Nectarines by the Western Flower Thrips (Thysanoptera: Thripidae) in the Interior of British Columbia, Canada

Damage to Nectarines by the Western Flower Thrips (Thysanoptera: Thripidae) in the Interior of British Columbia, Canada

Feeding behavior of the vectorDelphacodes kuscheli(Hemiptera: Fulgoromorpha: Delphacidae) on maize and oat

Role of Leaf Sheath Lignification and Anatomy in Resistance Against Southern Chinch Bug (Hemiptera: Blissidae) in St. Augustinegrass

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