Technical note The formulation of synthetic domestic wastewater sludge medium to study anaerobic biological treatment of acid mine drainage in the laboratory

Berg, Matthew; Botes, Marelize; Cloete, T.E.

doi:10.4314/wsa.v42i2.18

Cited by 6 publications

(3 citation statements)

References 13 publications

(5 reference statements)

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“…Therefore, in a preliminary study, synthetic domestic wastewater sludge (SDWWS) was formulated (Table 1) and a 1:1 ratio of AMD to SDWWS was determined as the optimum ratio for sulphate and COD removal (Van den Berg et al, 2016). All the components were purchased from Sigma-Aldrich (Pty) Ltd., Aston Manor, South Africa.…”

Section: Methodsmentioning

confidence: 99%

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Evaluating sulphate removal and identifying the bacterial community present in acid mine drainage treated with synthetic domestic wastewater sludge

Berg

Botes

Slabbert

et al. 2016

WSA

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Domestic wastewater sludge can serve as a carbon source in the passive biotic treatment of acid mine drainage (AMD) in microbial bioreactors to create anaerobic conditions for the removal of sulphate, chemical oxygen demand (COD) and pH neutralization. A synthetic medium simulating domestic wastewater sludge was used in AMD treatment in a ratio of 1:1 AMD: synthetic domestic wastewater sludge (SDWWS). Sulphate and COD removal were determined at different incubation temperatures and with and without a biofilm in the bioreactors. Sulphate and COD were removed by 60.8% and 96% within 26 d, after which a plateau was reached. Bacterial community analyses using next generation sequencing showed that Chlorobium spp. dominated at a relative percentage of 36% followed by Magnetospirillum spp. and Ornithobacterium spp. The effect of a resident biofilm in the bioreactors showed dominance of Chlorobium spp. at a relative percentage of 62% and removal of sulphates and COD at 96% and 58%, respectively, after 26 d. Incubation at 17-19°C reduced sulphates by only 10% and COD by 12% after 17 d, after which a plateau was reached. Magnetospirillum spp. was the dominate organism at the end of this trial.

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Section: Methodsmentioning

confidence: 99%

“…3). They are also present in domestic wastewater treatment plants (Valle et al, 2004;Hesham et al, 2011). The presence of Magnetospirillum spp.…”

Section: Sequence Analyses Of the 30 D And 30 D Biofilm Trialsmentioning

confidence: 99%

Evaluating sulphate removal and identifying the bacterial community present in acid mine drainage treated with synthetic domestic wastewater sludge

Berg

Botes

Slabbert

et al. 2016

WSA

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“…To prepare the application for wastewater samples, a synthetic wastewater sample was modified from the reported composition of synthetic domestic wastewater sludge by eliminating food wastes compositions (Table 3.1). 282 The synthetic MONPs wastewater was prepared by dispersing 10 mg/L of MONPs (2.5 mg/L of TiO2, CeO2, CuO and Al2O3) in total to the synthetic wastewater.…”

Section: Interference Studiesmentioning

confidence: 99%

Selective Detection and Removal of Zinc Oxide Nanoparticles in Contaminated Water

Zhang¹

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Metal oxide nanoparticles (MONPs) are massively produced for various industrial, environmental and biomedical applications due to their high surface reactivity and unique chemical and physical properties. Among all the manufactured MONPs, human exposure to zinc oxide (ZnO) nanoparticles may cause significant health concerns. This leads to a desire for efficient methods for their facile detection in the aqueous environment. In this work, two detection methods and one removal method were developed to selectively detect or remove ZnO nanoparticles in contaminated water with the presence of other MONPs.A detection method based on the fluorescence quenching of meso-tetra(4carboxyphenyl) porphyrin (TCPP) has been developed to treat water samples containing MONPs. Quenching of the TCPP emission intensity at 650 nm provides a Stern-Volmer plot with adequate sensitivity for the detection of 0.15 mg/mL ZnO nanoparticles.Meanwhile, a unique emission peak at 605 nm is observed and can be used for the identification and quantitation of ZnO nanoparticles down to 0.0015 mg/mL. A removal method using 3-aminopropyltriethoxysilane (APTES) has been proved to sediment ZnO nanoparticles in aqueous suspension with TCPP as a color indicator for ZnO. When 2.0% (v) APTES is applied to treat water samples containing ≥ 0.5 mg/mL of ZnO nanoparticles, a web-like sediment adheres onto the glass vial bottom from APTES-ZnO conjugation. A high removal efficiency of over 99% (w) ZnO nanoparticles was attained using APTES.Another detection method has been developed using electroanalytical analysis by firstly drop-casting MONPs on a screen-printed electrode. When phenol is analyzed as a iii chemical probe by cyclic voltammetry (CV), measurement of the reduction current provides adequate sensitivity for the indirect quantitation of 0.1 mg/mL ZnO nanoparticles.Both the oxidation peak and charge storage capacity measured from the cyclic voltammogram are proportional to the ZnO nanoparticle concentration and can afford a better detection limit of 0.01 mg/mL. Overall, the above methods are labour and cost effective and can afford high selectivity towards ZnO detection or removal in the presence of other MONPs.Additionally, the electrochemical analysis method and the APTES sedimentation method can differentiate ZnO nanoparticles from Zn 2+ and zinc peroxide (ZnO2) nanoparticles.iv PrefaceThis preface provides full bibliographical details for each article included in this thesis, as well as whether the article is reproduced in whole or in part. Permission was obtained from the publishers to include the articles in the thesis. When citing material from this thesis, please cite the article relevant to the chapter, if the chapter is based on a publication.Pursuant to the Integrated Thesis policy of Carleton University, the "supervisor" (Edward P.C. Lai) and the "student" (Wenyu Zhang) confirm that the student was fully involved in setting up and conducting the research, obtaining data and analyzing results, as well as preparing and writing the material pres...

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Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain

Ilić,

Davidović,

Milić

et al. 2024

Environ Sci Pollut Res

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Technical note The formulation of synthetic domestic wastewater sludge medium to study anaerobic biological treatment of acid mine drainage in the laboratory

Cited by 6 publications

References 13 publications

Evaluating sulphate removal and identifying the bacterial community present in acid mine drainage treated with synthetic domestic wastewater sludge

Evaluating sulphate removal and identifying the bacterial community present in acid mine drainage treated with synthetic domestic wastewater sludge

Selective Detection and Removal of Zinc Oxide Nanoparticles in Contaminated Water

Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain

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