Evaluating sulphate removal and identifying the bacterial community present in acid mine drainage treated with synthetic domestic wastewater sludge

Berg, Matthew; Botes, Marelize; Slabbert, Etienne; Cloete, T.E.

doi:10.4314/wsa.v42i3.13

Cited by 9 publications

(2 citation statements)

References 47 publications

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“…The third highest abundance was Sulfurospirillum. Although not known as pathogenic, they thrive in polluted habitats as their substrate range largely involves toxic compounds such as arsenate (van den Berg et al 2016). Next is Rombutsia, a newly discovered Gram-positive member of Peptostreptococcaceae.…”

Section: Resultsmentioning

confidence: 99%

Characterisation of Microbial Community Dynamics and Antibiotic Resistance Gene Dissemination in Malaysian Wastewater during the COVID-19 Pandemic

Shahid,

Li,

Yong

et al. 2024

Preprint

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Wastewater is a well-known hotspot for pathogens and spread of antibiotic resistance across species. Surveillance of wastewater microbial community can help draw clearer representation of actively culturing taxonomic groups and resistance-inducing mobile genetic elements before and after treatment. Studies have suggested that COVID-19 pandemic may also have caused increased dissemination of antibiotic-resistance genes (ARGs) and antibiotic-resistant bacteria in wastewater. Although immensely significant, no research has yet been performed on Malaysian wastewater microbial community and ARGs or their correlation with COVID-19 infections. This study utilised 16S metagenomics approach to characterise microbial community in Malaysian wastewater during high and low-case phases of pandemic. Among 20 most prevalent genera around Kuala Lumpur, Malaysia, those belonging to Bacteriodales, Bacillales, Actinomycetales and opportunistic pathogens-Arcobacters, Flavobacteria, and Campylobacterales, Neisseriales, were enriched during high-case periods of the COVID-19 pandemic. Copy number profiling of ARGs in water samples showed prevalence of elements conferring resistance to antibiotics like sulphonamides, cephalosporins, and colistin. High prevalence of intI1 and other ion-based transporters in samples highlight an extensive risk of horizontal gene transfer to previously susceptible species. Our study emphasises the importance of wastewater surveillance in understanding microbial community dynamics and ARG dissemination, particularly during public health crises like the COVID-19 pandemic.

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Section: Resultsmentioning

confidence: 99%

Characterisation of Microbial Community Dynamics and Antibiotic Resistance Gene Dissemination in Malaysian Wastewater during the COVID-19 Pandemic

Shahid,

Li,

Yong

et al. 2024

Preprint

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“…2FeS2(s) + 702(g) + 2H20(l) = 2Fe 2+ (aq) + 4SO4 2-(aq) + 4H + (aq) (1) The formation of sulphuric acid decreases the pH of the water. This lead to complete oxidation of pyrite to ferric iron and precipitation of ferric hydroxide termed yellow boy [3,4].…”

Section: Introductionmentioning

confidence: 99%

Laboratory Remediation of Iron-Sulphate Contaminant in Acid Mine Waters Using Waste Rocks

Oke¹,

Purchase²,

Mokitlane³

2019

Proceedings of the 5th World Congress on New Technologies

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In this paper, laboratory techniques of removing acid mine drainage rich in iron and sulphate is highlighted. AMD are contaminated water generated from mining of metals rich ore bodies such as pyrites and other iron and sulphide rich minerals. Three waste rocks of shale, bentonite and a mixture rock (shale-bentonite-calcrete) were used in removing the iron-sulphate concentration. Physico-chemical remediating process was carried out in the laboratory targeting the removal of the iron and sulphate in the AMD using these waste rocks. The remediation involved grinding the waste rocks into different particle sizes and sorting the grains using ASTM standard. The sorted waste rocks were arranged into three transparent bottle with regulated dip end. Each waste rock was placed into a distinct bottle. AMD rich in iron-sulphate was sourced from an abandoned mine. The iron-sulphate concentration in the AMD was determined using ICP-MS. The iron-sulphate concentration was determined before and after the remediation. Result show iron concentration in the AMD was 253 mg/l before remediation and 0.08 mg/l, 0.02 mg/l, 0.80 mg/l after remediation for bentonite, mixture and shale respectively. Likewise, sulphate concentration before remediation was 5067 mg/l before remediation and 3207 mg/l, 3662 mg/l, 2238 mg/l after remediation for bentonite, mixture and shale respectively. The laboratory experimentation has shown that controlled laboratory techniques can be used in removal of iron and minimise sulphate contained in AMD. The use of waste rocks of shale or bentonite with variety of grain size contents will remove iron-sulphate contamination and if use as liners in a constructed wetland will minimise the generation of AMD.

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Bioremediation of acid mine drainage – Review

Anekwe

Isa²

2023

Alexandria Engineering Journal

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Evaluating sulphate removal and identifying the bacterial community present in acid mine drainage treated with synthetic domestic wastewater sludge

Cited by 9 publications

References 47 publications

Characterisation of Microbial Community Dynamics and Antibiotic Resistance Gene Dissemination in Malaysian Wastewater during the COVID-19 Pandemic

Characterisation of Microbial Community Dynamics and Antibiotic Resistance Gene Dissemination in Malaysian Wastewater during the COVID-19 Pandemic

Laboratory Remediation of Iron-Sulphate Contaminant in Acid Mine Waters Using Waste Rocks

Bioremediation of acid mine drainage – Review

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