Technical Note: Measurement of Total Estrone Content in Foods. Application to Dairy Products

García‐Peláez, Beatriz; Ferrer-Lorente, Raquel; Gómez-Ollés, Susana; Fernández-López, José Antonio; Remesar, Xavier; Alemany, M

doi:10.3168/jds.s0022-0302(04)73354-8

Cited by 16 publications

(19 citation statements)

References 16 publications

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“…Feed intake is positively correlated with milk production; so higher producing cows would have a greater liver blood flow and, therefore, higher metabolic clearance rate of these compounds as well. Cows in our study had much higher milk yields than those in the European reports [16][17][18]24,31 and cows of the 1970s and 1980s. 32,33 Consequently, the E 1 values in our experiments are lower than the 20 to 56 pg/mL (73.98 to 207.1 pmol/L) reported in earlier studies.…”

Section: Resultscontrasting

confidence: 69%

“…32,33 Consequently, the E 1 values in our experiments are lower than the 20 to 56 pg/mL (73.98 to 207.1 pmol/L) reported in earlier studies. 10,12,[16][17][18]24,31 Concentrations of E 1 S were higher than those for free E 1 in our study with 87% of total E 1 (E 1 ϩE 1 S) being present in the conjugated form. This is similar to the 85% to 96% values reported by others.…”

Section: Resultscontrasting

confidence: 67%

“…10,13,14 The methodology used in our study effectively separated E 1 from other estrogens and cholesterol. Even a small cross-reactivity of the E 1 antibody with the milligrams per milliliter concentrations of cholesterol in milk could lead to greatly inflated values for E 1 and E 1 S. In the two studies reporting nanograms per milliliter concentrations of total E 1 , 16,17 it was unclear whether cholesterol was removed from the samples before analysis.…”

Section: Resultsmentioning

confidence: 93%

See 2 more Smart Citations

Estrone and Estrone Sulfate Concentrations in Milk and Milk Fractions

Macrina¹,

Ott²,

Roberts³

et al. 2012

Journal of the Academy of Nutrition and Dietetics

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Section: Resultscontrasting

confidence: 69%

Section: Resultscontrasting

confidence: 67%

Section: Resultsmentioning

confidence: 93%

See 1 more Smart Citation

Estrone and Estrone Sulfate Concentrations in Milk and Milk Fractions

Macrina¹,

Ott²,

Roberts³

et al. 2012

Journal of the Academy of Nutrition and Dietetics

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“…A modified method was used for the analysis of acyl-estrone in foods [26]. A much improved version of this method was developed for the estimation of acyl-estrone in dairy products [27]. An antibody that does not react with estradiol was used to measure the evolved estrone and allowed us to assign the levels of "acyl-estrone" measured to OE.…”

Section: Oe Methodologymentioning

confidence: 99%

Oleoyl-estrone is a precursor of an estrone-derived ponderostat signal

Vila

Cabot

Villarreal

et al. 2011

The Journal of Steroid Biochemistry and Molecular Biology

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Oleoyl-estrone (OE) is a powerful anti-obesity compound that decreases food intake, decreases insulin resistance and circulating cholesterol. OE stimulates a severe loss of body fat by decreasing adipose tissue lipid synthesis and maintaining lipolysis. Therefore, the body economy loses lipid energy because energy expenditure is maintained. This study analyses the discrepancy between OE effects and the distribution of labelled OE in plasma. Estrone radioimmunoassay of organic solvent plasma extracts of rats treated with OE showed the massive presence of acyl-estrone, but saponification did not release estrone, but containing similar unknown compound. Analysis of label distribution in plasma after oral gavages of (3)H-OE showed the presence of a more hydrophilic compound than OE or any estrogen as well as (3)H(2)O, formed from (3)H-OE in the acidic stomach medium. OE was not attached to a specific transporter in plasma. Through serum HPLC analysis we found W, a labelled derivative more hydrophilic than OE or estrone. The results were confirmed using (14)C-OE. HPLC-MS/MS studies showed that plasma OE levels were one order of magnitude lower than those of W. When liver cell cytosols from rats laden with (3)H-OE were incubated with nuclei from untreated rats, the OE-derived label (i.e., Ws) was found attached to nuclear DNA. Neither estradiol nor estrone interfered with its binding. W is a fairly hydrophilic compound of low molecular weight containing the estrone nucleus, but it is not an ester because saponification or esterases do not yield estrone as OE does. It is concluded that OE acts through its conversion to W, its active form; which binds to a nuclear receptor different from that of estrogen. The estimated W serum levels are proportional to the pharmacological OE effects in vivo. We postulate W as a new type of hormone that exerts the full range of in vivo effects thus far attributed to OE. The full identification of W is anticipated to open the way for the development of new OE-like anti-obesity drugs.

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“…Although milk contains bovine IGF-I, which is structurally identical to human IGF-I (19), and rat studies have suggested that there is intact absorption (20), it is generally assumed that IGF-I does not retain bioactivity when consumed orally because of the rapid proteolysis in the upper gut. Milk and dairy products also contain measurable amounts of steroid hormones (21)(22)(23), which may influence breast density or pubertal timing (24). Dairy consumption has also been linked to total fat intake, which may, in turn, be associated with breast cancer risk (25).…”

Section: Introductionmentioning

confidence: 99%

Dairy intake in relation to breast and pubertal development in Chilean girls,

Gaskins

Pereira

Quintiliano

et al. 2017

The American Journal of Clinical Nutrition

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Background: Frequent dairy consumption in childhood has been related to higher growth-hormone concentrations that may affect mammary gland and pubertal development. Objective: We evaluated the relation of dairy intake to breast composition at Tanner stage 4 and age at menarche. Design: A total of 515 Chilean girls are included in the Growth and Obesity Cohort Study. The subjects have been followed longitudinally since they were 3-4 y old (from 2006 to the present). Starting in 2013, diet was assessed every 6 mo via a 24-h recall. The breast fibroglandular volume (FGV) was measured with the use of dual-energy X-ray absorptiometry at Tanner stage 4. The date of menarche was reported every 6 mo. Our analysis included 290 girls with data on prospective diet and breast composition and 324 girls with data on prospective diet and age at menarche. Results: The mean 6 SD breast FGV and percentage of fibroglandular volume (%FGV) (i.e., FGV divided by total breast volume times 100) at Tanner stage 4 was 81.7 6 32.2 cm 3 and 42.0% 6 16.7%, respectively. Only sweetened, artificially flavored milk-based drinks were associated with the %FGV with girls who consumed .125 g/d having a %FGV that was 4.5% (95% CI: 0.9%, 8.1%) higher than that of girls who consumed none (P-trend = 0.007). Yogurt intake was associated with a lower FGV. Specifically, girls who consumed .125 g yogurt/d had 210.2 cm 3 (95% CI: 220.2, 20.3 cm 3 ) less FGV than did girls who consumed no yogurt (P-trend = 0.03). The majority (90.7%) of girls in our cohort attained menarche before the data analyses with a mean 6 SD age at menarche of 11.9 6 0.7 y. In multivariable models, low-fat dairy, low-fat milk, and yogurt intakes were associated with a later age at menarche. In particular, girls who consumed .125 g yogurt/d had menarche, on average, 4.6 mo (95% CI: 1.9, 7.4 mo) later than girls who consumed no yogurt (P-trend = 0.01). Conclusion: More-frequent consumption of sweetened, artificiallyflavored milk-based drinks is associated with a higher %FGV, whereas higher yogurt intake is associated with a lower FGV and delayed age at menarche in Chilean girls.Am J Clin Nutr 2017;105:1166-75.

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Technical Note: Measurement of Total Estrone Content in Foods. Application to Dairy Products

Cited by 16 publications

References 16 publications

Estrone and Estrone Sulfate Concentrations in Milk and Milk Fractions

Estrone and Estrone Sulfate Concentrations in Milk and Milk Fractions

Oleoyl-estrone is a precursor of an estrone-derived ponderostat signal

Dairy intake in relation to breast and pubertal development in Chilean girls,

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