Technical and Software Advances in Bacterial Pathogen Typing

Chui, Linda; Li, Vincent

doi:10.1016/bs.mim.2015.06.003

Cited by 2 publications

(1 citation statement)

References 166 publications

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“…The standardization of MLST data allows users to investigate the molecular evolution of pathogens over time in different geographic regions. For outbreaks surveillance and management, MLST being able to rapidly keep track of infectious diseases is of paramount importance ( Chui and Li, 2015 ). MLST is also a technically straightforward typing tool and provides unambiguous data that is highly reproducible between laboratories ( Pérez-Losada et al, 2011 ).…”

Section: Molecular Typing Methods Of Pseudomonas Aeruginosamentioning

confidence: 99%

Recent Advances in Molecular Diagnosis of Pseudomonasaeruginosa Infection by State-of-the-Art Genotyping Techniques

et al. 2018

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Pseudomonas aeruginosa is a rod-shaped Gram-negative bacterium which is notably known as a pathogen in humans, animals, and plants. Infections caused by P. aeruginosa especially in hospitalized patients are often life-threatening and rapidly increasing worldwide throughout the years. Recently, multidrug-resistant P. aeruginosa has taken a toll on humans’ health due to the inefficiency of antimicrobial agents. Therefore, the rapid and advanced diagnostic techniques to accurately detect this bacterium particularly in clinical samples are indeed necessary to ensure timely and effective treatments and to prevent outbreaks. This review aims to discuss most recent of state-of-the-art molecular diagnostic techniques enabling fast and accurate detection and identification of P. aeruginosa based on well-developed genotyping techniques, e.g., polymerase chain reaction, pulse-field gel electrophoresis, and next generation sequencing. The advantages and limitations of each of the methods are also reviewed.

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Section: Molecular Typing Methods Of Pseudomonas Aeruginosamentioning

confidence: 99%

Recent Advances in Molecular Diagnosis of Pseudomonasaeruginosa Infection by State-of-the-Art Genotyping Techniques

et al. 2018

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Moving towards improved surveillance and earlier diagnosis of aquatic pathogens: From traditional methods to emerging technologies

MacAulay

Ellison

Kille

et al. 2022

Reviews in Aquaculture

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Early and accurate diagnosis is key to mitigating the impact of infectious diseases, along with efficient surveillance. This however is particularly challenging in aquatic environments due to hidden biodiversity and physical constraints. Traditional diagnostics, such as visual diagnosis and histopathology, are still widely used, but increasingly technological advances such as portable next generation sequencing (NGS) and artificial intelligence (AI) are being tested for early diagnosis. The most straightforward methodologies, based on visual diagnosis, rely on specialist knowledge and experience but provide a foundation for surveillance. Future computational remote sensing methods, such as AI image diagnosis and drone surveillance, will ultimately reduce labour costs whilst not compromising on sensitivity, but they require capital and infrastructural investment. Molecular techniques have advanced rapidly in the last 30 years, from standard PCR through loop-mediated isothermal amplification (LAMP) to NGS approaches, providing a range of technologies that support the currently popular eDNA diagnosis. There is now vast potential for transformative change driven by developments in human diagnostics. Here we compare current surveillance and diagnostic technologies with those that could be used or developed for use in the aquatic environment, against three gold standard ideals of high sensitivity, specificity, rapid diagnosis, and cost-effectiveness.

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Technical and Software Advances in Bacterial Pathogen Typing

Cited by 2 publications

References 166 publications

Recent Advances in Molecular Diagnosis of Pseudomonasaeruginosa Infection by State-of-the-Art Genotyping Techniques

Recent Advances in Molecular Diagnosis of Pseudomonasaeruginosa Infection by State-of-the-Art Genotyping Techniques

Moving towards improved surveillance and earlier diagnosis of aquatic pathogens: From traditional methods to emerging technologies

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