2018
DOI: 10.3389/fmicb.2018.01104
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Recent Advances in Molecular Diagnosis of Pseudomonasaeruginosa Infection by State-of-the-Art Genotyping Techniques

Abstract: Pseudomonas aeruginosa is a rod-shaped Gram-negative bacterium which is notably known as a pathogen in humans, animals, and plants. Infections caused by P. aeruginosa especially in hospitalized patients are often life-threatening and rapidly increasing worldwide throughout the years. Recently, multidrug-resistant P. aeruginosa has taken a toll on humans’ health due to the inefficiency of antimicrobial agents. Therefore, the rapid and advanced diagnostic techniques to accurately detect this bacterium particular… Show more

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Cited by 29 publications
(25 citation statements)
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“…The cgMLST scheme proposed here helped to discriminate isolates belonging to the same ST, while clearly grouping isolates that had been epidemiologically linked in the original publications. Even though MLST is widely used in epidemiological studies, the fact that it addresses less that 0.1% of the genome, often limits its discriminatory power (Li et al, 2009;Sabat et al, 2013;Davis et al, 2015;Tang et al, 2017;Chen et al, 2018;Ghanem and El-Gazzar, 2018;Kimura, 2018). Our results are in agreement with recent publications for pathogens such as Enterococcus faecium, L. monocytogenes, A. baumannii, K. pneumoniae, and E. faecalis (de Been et al, 2015;Ruppitsch et al, 2015;Higgins et al, 2017;Zhou et al, 2017;Neumann et al, 2019).…”
Section: Discussionsupporting
confidence: 91%
“…The cgMLST scheme proposed here helped to discriminate isolates belonging to the same ST, while clearly grouping isolates that had been epidemiologically linked in the original publications. Even though MLST is widely used in epidemiological studies, the fact that it addresses less that 0.1% of the genome, often limits its discriminatory power (Li et al, 2009;Sabat et al, 2013;Davis et al, 2015;Tang et al, 2017;Chen et al, 2018;Ghanem and El-Gazzar, 2018;Kimura, 2018). Our results are in agreement with recent publications for pathogens such as Enterococcus faecium, L. monocytogenes, A. baumannii, K. pneumoniae, and E. faecalis (de Been et al, 2015;Ruppitsch et al, 2015;Higgins et al, 2017;Zhou et al, 2017;Neumann et al, 2019).…”
Section: Discussionsupporting
confidence: 91%
“…Pseudomonas (P.) aeruginosa , a Gram-negative oxidase-positive rod shaped, motile bacterium is associated with acute and chronic infections in various species. In humans, P. aeruginosa is considered an opportunistic pathogen responsible for high morbidity and mortality infection rates leading to pneumonia, septicemia, and necrosis in immunocompromised patients [ 1 ]. Because of high antimicrobial resistance rates, the World Health Organization [ 2 ] additionally lists it as a critical priority pathogen.…”
Section: Introductionmentioning
confidence: 99%
“… 8 Also, polymerase chain reaction (PCR) methods have appeared as interesting alternatives to identify pathogens. 9 However, these techniques require complex selection of the targets, tedious and time-consuming extractions, highly trained personnel, and/or expensive equipment, 10 which prevent their wide implementation on clinical settings. Point of care (PoC) devices may be a promising option in future routine clinical diagnostics, providing rapid reliable responses and being low cost and user-friendly to use.…”
mentioning
confidence: 99%