Technical advancement in plant virus diagnosis – an appraisal

Abstract

Plants infected by a wide range of viruses often cause important agronomic and economic losses worldwide. Detection of plant viruses is becoming more challenging as globalisation of trade, particularly the ornamental plants, and the potential effects of climate change facilitate movement of viruses and their vectors, thereby transforming the diagnostic landscape. Under such circumstances, detection of viruses at premature stages of infection by use of rapid, sensitive and accurate detection methods seems crucial to ensure safe and sustainable agriculture and as such reduces economic losses. For proper identification, detection technique needs to be rapid, most accurate and inexpensive, as it forms the key step in developing appropriate practical solutions to manage plant virus diseases that pose continuous threats to the viability of certain horticultural and agricultural industries. This review is concerned with the advancement in techniques in the diagnosis of viruses in plants.

Viruses Infecting Trees and Herbs That Produce Edible Fleshy Fruits with a Prominent Value in the Global Market: An Evolutionary Perspective

“…Representatives of the four main virus families including Betaflexiviridae, Closteroviridae, Secoviridae, and Tymoviridae were found alone and in combination [347]. The main limitations of this technique are the high cost of spotting, need for labeled nucleotides, need for dust-free rooms, and little flexibility for use in differentiating strains, as well as the time for processing data [348]. 6.2.9.…”