Technetium 99m Autoradiography of Labelled White Cells

Barbu, M.; Colas-Linhart, N.; B, Bok

doi:10.1159/000206498

Cited by 7 publications

(1 citation statement)

References 5 publications

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“…Radiolabelled leucocytes represent a relatively straightforward subject for study by autoradiography since they are readily available and can be labelled in vitro. 99mTc-HMPAO labelled leucocytes were studied [84,85] using track autoradiography on fixed smears of whole leucocytes. The distribution among the cells was highly heterogeneous: 75% of the cells exhibited virtually no labelling (less than three tracks per cell) whereas 10% of the cells were heavily labelled (>20 tracks per cell).…”

Section: Targeting Mechanismsmentioning

confidence: 99%

Radionuclide targeting and dosimetry at the microscopic level: the role of microautoradiography

Puncher

Blower

1994

Eur J Nucl Med

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The understanding of localisation mechanisms and microdosimetry of diagnostic and therapeutic radiopharmaceuticals depends on knowledge of their biodistribution at the microscopic level (cellular and subcellular) in the target tissues. Various methods have been advanced for obtaining information about this microdistribution: subcellular fractionation, secondary ion mass spectrometry imaging, microprobe elemental analysis in the electron microscope, and microautoradiography. This review compares these approaches, and discusses in detail the methodology of microautoradiography (the most generally useful approach) with imaging and therapy radionuclides. Literature examples of applications of microautoradiography in nuclear medicine are reviewed, and the future potential contribution of the techniques is assessed.

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Section: Targeting Mechanismsmentioning

confidence: 99%

Radionuclide targeting and dosimetry at the microscopic level: the role of microautoradiography

Puncher

Blower

1994

Eur J Nucl Med

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Cell Irradiation caused by diagnostic nuclear medicine procedures: dose heterogeneity and biological consequences

Gardin

Faraggi

Guludec

et al. 1999

European Journal of Nuclear Medicine and Molecular Imaging

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Most radionuclides used for diagnostic imaging emit Auger electrons (technetium-99m, iodine-123, indium-111, gallium-67 and thallium-201). Their very short range in biological tissues may lead to dose heterogeneity at the cellular level with radiobiological consequences. This report describes the dosimetric models used to calculate the mean dose absorbed by the cell nucleus from Auger radionuclides. The techniques used to determine the biodistribution of radiopharmaceuticals at the subcellular level are also described and compared. Published examples of cellular dosimetry computations performed with radiotracers are reviewed in various clinical settings. Finally, the biological implications of the subcellular localization of Auger emitters are examined. While a number of efforts have been made to obtain dosimetric models and to estimate subcellular distribution of radioactivity, little is known of the cellular dosimetry of most radiopharmaceuticals used in diagnostic imaging. However, biological examples of selective radiotracer uptake have been shown, leading to extremely strong cell-cell dose heterogeneity. Furthermore, radiobiological experiments show that the biological effects of Auger emitters incorporated into DNA can be severe, with relative biological effectiveness greater than 1 compared with external X-rays. These findings clearly show that the assessment of biological risks associated with internal administration of diagnostic radiopharmaceuticals must focus not only on target organs as a whole, but also on the cellular level. This review proposes the most appropriate model for dosimetric computations (cellular or conventional) according to the subcellular distribution of radiotracers. The radionuclide of choice and the general strategy used to design new diagnostic radiopharmaceuticals are also discussed.

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Phagocytic labeling of leukocytes with 99mTc-albumin colloid for nuclear imaging

Marcus

Kuperus

Butler

et al. 1988

International Journal of Radiation Applications and Instrumenta

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Technetium 99m Autoradiography of Labelled White Cells

Cited by 7 publications

References 5 publications

Radionuclide targeting and dosimetry at the microscopic level: the role of microautoradiography

Radionuclide targeting and dosimetry at the microscopic level: the role of microautoradiography

Cell Irradiation caused by diagnostic nuclear medicine procedures: dose heterogeneity and biological consequences

Phagocytic labeling of leukocytes with 99mTc-albumin colloid for nuclear imaging

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