Teaching report: the use of Drosophila melanogaster larval thermosensitive escape behaviour as a model system to demonstrate sensory function

Harrison, A.B.; Oswald, Matthew; Sweeney, Sean T.

doi:10.1007/s10158-011-0123-4

Cited by 2 publications

(2 citation statements)

References 10 publications

(7 reference statements)

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“…Along these lines, the scope of teaching about Drosophila can be taken a level further by having a more prominent presence on school curricula or on public outreach events. Increasingly, examples of such activities are being published (Harrison et al 2011; Pulver et al 2011), and aspects of the training material published here could provide further useful ideas for content and illustration for school teaching or outreach activities.…”

Section: Discussionmentioning

confidence: 96%

How to Design a Genetic Mating Scheme: A Basic Training Package for Drosophila Genetics

Roote

Prokop

2013

G3 Genes|Genomes|Genetics

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Drosophila melanogaster is a powerful model organism for biological research. The essential and common instrument of fly research is genetics, the art of applying Mendelian rules in the specific context of Drosophila with its unique classical genetic tools and the breadth of modern genetic tools and strategies brought in by molecular biology, transgenic technologies and the use of recombinases. Training newcomers to fly genetics is a complex and time-consuming task but too important to be left to chance. Surprisingly, suitable training resources for beginners currently are not available. Here we provide a training package for basic Drosophila genetics, designed to ensure that basic knowledge on all key areas is covered while reducing the time invested by trainers. First, a manual introduces to fly history, rationale for mating schemes, fly handling, Mendelian rules in fly, markers and balancers, mating scheme design, and transgenic technologies. Its self-study is followed by a practical training session on gender and marker selection, introducing real flies under the dissecting microscope. Next, through self-study of a PowerPoint presentation, trainees are guided step-by-step through a mating scheme. Finally, to consolidate knowledge, trainees are asked to design similar mating schemes reflecting routine tasks in a fly laboratory. This exercise requires individual feedback but also provides unique opportunities for trainers to spot weaknesses and strengths of each trainee and take remedial action. This training package is being successfully applied at the Manchester fly facility and may serve as a model for further training resources covering other aspects of fly research.

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Section: Discussionmentioning

confidence: 96%

How to Design a Genetic Mating Scheme: A Basic Training Package for Drosophila Genetics

Roote

Prokop

2013

G3 Genes|Genomes|Genetics

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“…Hewan invertebrata banyak dipakai sebagai alternatif pengganti penggunaan hewan untuk penelitian dasar fungsi biologi manusia, sebagai contoh mempelajari perkembangan penyakit Alzheimer, neurodegenerative, aspek sensasi nyeri digunakan lalat buah Drosophila (Harrison et al 2010). Penggunaan jamur lendir Dictyostelium pada penelitian tentang epilepsi dan gangguan bipolar juga telah dilaporkan (Williams et al 2006).…”

Section: Replacementunclassified

Animal Welfare Ethics in Research and Testing: Implementation and its Barrier

2020

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Animals have an important role in research and testing to improve human and animal health. Animal usage must be balanced between science and ethical values of animal welfare. This paper discusses the role of IACUC, the implications of animal welfare for research, animal ethical clearance and obstacles in the implementation of animal welfare. Institution of Animal Care Use Committee (IACUC) has an important role to ensure that researcher has animal ethical clearance before conducting research and testing. Research and testing using animal should comply with ethical principles: respect, beneficiary and justice; 3Rs principles: Replacement, Reduction, Refinement and 5F/Freedom: freedom from hunger and thirst, heat and discomfort, pain, trauma and disease, fear and stress and expressing behavior naturally. The application of animal ethics clearance in Indonesia in research using animals is compulsary in various institutions, However thera are several barriers in its implementation, Those are: not all research institutions have IACUC, lack of awareness of researchers to apply for animal ethical clearance, reluctant to IACUC requirements, lack of facilities animals that meet animal welfare requirements and lack of competence in animal handling according to animal welfare.

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Teaching report: the use of Drosophila melanogaster larval thermosensitive escape behaviour as a model system to demonstrate sensory function

Cited by 2 publications

References 10 publications

How to Design a Genetic Mating Scheme: A Basic Training Package for Drosophila Genetics

How to Design a Genetic Mating Scheme: A Basic Training Package for Drosophila Genetics

Animal Welfare Ethics in Research and Testing: Implementation and its Barrier

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