Tea polyphenols inhibits biofilm formation, attenuates the quorum sensing-controlled virulence and enhances resistance to Klebsiella pneumoniae infection in Caenorhabditis elegans model

Liu, Wugao; Lu, Hongjia; Chu, Xinyu; Lou, Tianzheng; Zhang, Ning; Bao, Zhang; Chu, Weihua

doi:10.1016/j.micpath.2020.104266

Cited by 26 publications

(15 citation statements)

References 33 publications

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“…Since the extraction was performed stepwise, a low activity for the water extracts is plausible as the suspected active compounds were already extracted with methanol. Since tannins and flavonoids are also known for antimicrobial activity [ 5 , 6 , 7 ], a correlation analysis between tannins, flavonoids, and biological activity was considered reasonable.…”

Section: Resultsmentioning

confidence: 99%

“…For flavonoids, no statistical significant correlation can be observed. This is somewhat remarkable, as flavonoids are commonly regarded as active biofilm-inhibiting substances [ 5 , 6 , 7 , 30 ]. There are two approaches to explain how the data correlation might have been biased resulting in differences from the literature data.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, some of the main survival and evolutionary successes of multi-resistant bacteria might be due to the capability of biofilm formation [ 4 ]. Tannin- and flavonoid-rich plants and drugs have been proven in the past to affect biofilms of bacteria [ 5 , 6 , 7 ]. On the other hand, it is known that some plant polyphenols, especially tannins, directly inhibit bacteria by denaturation of bacterial proteins [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

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Evaluating Tannins and Flavonoids from Traditionally Used Medicinal Plants with Biofilm Inhibitory Effects against MRGN E. coli

et al. 2022

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In the search for alternative treatment options for infections with multi-resistant germs, traditionally used medicinal plants are currently being examined more intensively. In this study, the antimicrobial and anti-biofilm activities of 14 herbal drugs were investigated. Nine of the tested drugs were traditionally used in Europe for treatment of local infections. For comparison, another five drugs monographed in the European Pharmacopoeia were used. Additionally, the total tannin and flavonoid contents of all tested drugs were analyzed. HPLC fingerprints were recorded to obtain further insights into the components of the extracts. The aim of the study was to identify herbal drugs that might be useable for treatment of infectious diseases, even with multidrug resistant E. coli, and to correlate the antimicrobial activity with the total content of tannins and flavonoids. The agar diffusion test and anti-biofilm assay were used to evaluate the antimicrobial potential of different extracts from the plants. Colorimetric methods (from European Pharmacopeia) were used for determination of total tannins and flavonoids. The direct antimicrobial activity of most of the tested extracts was low to moderate. The anti-biofilm activity was found to be down to 10 µg mL−1 for some extracts. Tannin contents between 2.2% and 10.4% of dry weight and total flavonoid contents between 0.1% and 1.6% were found. Correlation analysis indicates that the antimicrobial and the anti-biofilm activity is significantly (p < 0.05) dependent on tannin content, but not on flavonoid content. The data analysis revealed that tannin-rich herbal drugs inhibit pathogens in different ways. Thus, some of the tested herbal drugs might be useable for local infections with multi-resistant biofilm-forming pathogens. For some of the tested drugs, this is the first report about anti-biofilm activity, as well as total tannin and flavonoid content.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluating Tannins and Flavonoids from Traditionally Used Medicinal Plants with Biofilm Inhibitory Effects against MRGN E. coli

et al. 2022

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“…Under the protection of EPS, microorganisms on biofilm are protected from the direct action of disinfection and have access to more nutrients. Liu et al [41] studied the effects of tea polyphenols on the quorum sensing and virulence factors of Klebsiella Pneumoniae and found that tea polyphenols inhibited population sensing activity and reduced their motility, protease and extracellular polysaccharide production, then inhibited biofilm formation. Huber et al [42] also reported that plant polyphenols could interfere with bacterial population sensing and inhibit bacterial biofilm formation.…”

Section: Microbial Diversity Analysis Based On Class Levelmentioning

confidence: 99%

Microbial Characteristics of the Combined Ozone and Tea Polyphenols or Sodium Hypochlorite Disinfection in the Pipe Network

Feng

Zhu

et al. 2021

Water

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Microbiological safety of water in the pipe network is an important guarantee for safe drinking water. Simulation tests of stainless steel pipe network were carried out using te4a polyphenols and sodium hypochlorite as auxiliary disinfectants for ozone disinfection to analyze the persistent disinfection effects of different combined disinfection methods by measuring the changes in total bacterial colonies in the water. High-throughput sequencing of microorganisms in the pipe network was performed to analyze the differences in the community structure of microorganisms in the water and pipe wall under different disinfection methods. The results showed that the application of auxiliary disinfectants had a relatively long-lasting inhibitory effect on the bacterial colonies in the water, and the diversity of microorganisms in the pipe network varied significantly. As an auxiliary disinfectant for ozone disinfection, tea polyphenols are more powerful than sodium hypochlorite in killing pathogens and chlorine-resistant bacteria, so they are more beneficial to ensure the microbiological safety of water in stainless steel pipe networks.

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“…Genes implicated in QS control various phenotypes, including bioluminescence, biofilm formation, drug resistance, virulence factors expression, and motility [279]. Recent studies have demonstrated that phenolic compounds are implicated in the process of regulating the bacteria growth by targeting the QS system [280,281]. Mostafa et al [282] detected 38 metabolites obtained from Salix tetrasperma extract that exhibit proteolytic, hemolytic, and motility inhibition in assays with P. aeruginosa.…”

Section: Anti-bacterialmentioning

confidence: 99%

Antioxidant Molecules from Plant Waste: Extraction Techniques and Biological Properties

et al. 2020

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The fruit, vegetable, legume, and cereal industries generate many wastes, representing an environmental pollution problem. However, these wastes are a rich source of antioxidant molecules such as terpenes, phenolic compounds, phytosterols, and bioactive peptides with potential applications mainly in the food and pharmaceutical industries, and they exhibit multiple biological properties including antidiabetic, anti-obesity, antihypertensive, anticancer, and antibacterial properties. The aforementioned has increased studies on the recovery of antioxidant compounds using green technologies to value plant waste, since they represent more efficient and sustainable processes. In this review, the main antioxidant molecules from plants are briefly described and the advantages and disadvantages of the use of conventional and green extraction technologies used for the recovery and optimization of the yield of antioxidant naturals are detailed; finally, recent studies on biological properties of antioxidant molecules extracted from plant waste are presented here.

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Tea polyphenols inhibits biofilm formation, attenuates the quorum sensing-controlled virulence and enhances resistance to Klebsiella pneumoniae infection in Caenorhabditis elegans model

Cited by 26 publications

References 33 publications

Evaluating Tannins and Flavonoids from Traditionally Used Medicinal Plants with Biofilm Inhibitory Effects against MRGN E. coli

Evaluating Tannins and Flavonoids from Traditionally Used Medicinal Plants with Biofilm Inhibitory Effects against MRGN E. coli

Microbial Characteristics of the Combined Ozone and Tea Polyphenols or Sodium Hypochlorite Disinfection in the Pipe Network

Antioxidant Molecules from Plant Waste: Extraction Techniques and Biological Properties

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