Tea catechins enhance the mRNA expression of uncoupling protein 1 in rat brown adipose tissue

Nomura, Sadahiro; Ichinose, Takashi; Jinde, Manabu; Kawashima, Yu; Tachiyashiki, Kaoru; Imaizumi, Kazunori

doi:10.1016/j.jnutbio.2007.11.005

Cited by 75 publications

(80 citation statements)

References 35 publications

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“…Animal chow (CE-2 cubic type, CLEA Japan, Inc.) and distilled water were given to the rats ad libitum. 19) After the adaptation period, the…”

Section: Methodsmentioning

confidence: 99%

“…Experiments were designed to minimize pain and discomfort for the rats. [19][20][21] Both experiments were approved by the Committee on Animal Care Use at Waseda University. Administration of Dexamethasone to Rats --Dexamethasone 21-phosphate (Sigma, St. Louis, MO, U.S.A.) was first dissolved in 0.9% NaCl solution as a vehicle to obtain a final dexamethasone concentration of 0.1%.…”

Section: Fig 1 Experimental Protocol Used In the Present Studymentioning

confidence: 99%

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Acute and Subacute Effects of Dexamethasone on the Number of White Blood Cells in Rats

Ohkaru

Arai

Ohno

et al. 2010

JOURNAL OF HEALTH SCIENCE

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The purpose of this study was to elucidate the effects of dexamethasone, a synthetic glucocorticoid, on the immune system by analyzing the number of white blood cells (WBCs) over the course of hours and days of dexamethasone administration. Dexamethasone was given as either a single dosage [1.0 mg/kg body weight (BW); subcutaneous injection (s.c.)] or as a daily dosage (1.0 mg/kg BW per day; s.c.) for 10 days for the hourly and daily assessment of changes in the number of white blood cells, respectively. A single administration of dexamethasone markedly decreased the number of total WBCs, as well as the number of lymphocyte, monocyte, neutrophil and eosinophil subsets with a nadir at 8 hr post-injection. The number of these cells recovered to the control levels at 24 hr. The numbers of total WBCs, lymphocytes, monocyte, eosinophil and basophil were reduced by the daily administration of dexamethasone. However, the number of neutrophil was significantly higher at days 2 and 8 after the injection. These results suggest that glucocorticoid-mediated immunosuppressions are at least partly attributable to quantitative changes in the number of circulating WBCs.

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“…Animal chow (CE-2 cubic type, CLEA Japan, Inc.) and distilled water were given to the rats ad libitum. 19) After the adaptation period, the…”

Section: Methodsmentioning

confidence: 99%

Section: Fig 1 Experimental Protocol Used In the Present Studymentioning

confidence: 99%

Acute and Subacute Effects of Dexamethasone on the Number of White Blood Cells in Rats

Ohkaru

Arai

Ohno

et al. 2010

JOURNAL OF HEALTH SCIENCE

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“…RNeasy Mini Spin Column, an adjunct with RNeasy Fibrous Tissue Mini Kit (Qiagen, Hilden, Germany), was used for the purification of RNA. The RNA concentration was determined by measuring absorbance at 260 and 280 nm (U-3310 Spectrophotometer; Hitachi, Tokyo) according to our routine method (32).…”

Section: Analyses Of Mrna Expressions By Real-time Quantitative Revermentioning

confidence: 99%

“…In quantitative RT-PCR, synthesized cDNA was added to a SYBR Premix Ex Taq reaction mixture (Takara Bio Inc., Shiga) containing 200 nM PCR primer (forward and reverse) (32). Primer sequences for real-time RT-PCR were β 1 -AR: 5'-CTG CTA CAA CGA CCC CAA GTG-3' (forward), 5'-AAC ACC CGG AGG TAC ACG AA-3' (reverse) and β 2 -AR: 5'-GAG CCA CAC GGG AAT GAC A-3' (forward), 5'-CCA GGA CGA TAA CCG ACA TGA-3' (reverse).…”

Section: Analyses Of Mrna Expressions By Real-time Quantitative Revermentioning

confidence: 99%

Effects of the β2-Agonist Clenbuterol on β1- and β2-Adrenoceptor mRNA Expressions of Rat Skeletal and Left Ventricle Muscles

et al. 2008

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Abstract. The β 2 -agonist clenbuterol [4-amino-α(t-butyl-amino)methyl-3,5-dichlorobenzyl alcohol] is used as a non-steroidal anabolic drug for sports doping. The effects of clenbuterol on the transcriptional process and mRNA stability of β-adrenoceptor (β-AR) in skeletal and cardiac muscles are still unknown. Therefore, we investigated the effects of clenbuterol on β 1 -and β 2 -AR mRNA expressions of fast-twitch fiber-rich extensor digitorum longus (EDL), slow-twitch fiber-rich soleus (SOL), and left ventricle (LV) muscles by real-time RT-PCR. Adult male Sprague Dawley rats were divided into the clenbuterol-administered group and control group. The administration (dose = 1.0 mg / kg body weight / day, s.c.) of clenbuterol was maintained for 10 days. The administration of clenbuterol significantly increased the weight, RNA concentration, and total RNA content of EDL muscle. No effects of clenbuterol on those of SOL and LV muscles, however, were observed. The administration of clenbuterol significantly decreased β 1 -AR mRNA expression of LV muscle. Furthermore, the administration of clenbuterol significantly decreased β 2 -AR mRNA expression of EDL and LV muscles. No effect of clenbuterol on β 2 -AR mRNA expression of SOL muscle, however, was observed. These results suggest that the effects of clenbuterol on β 1 -and β 2 -AR mRNA expressions and muscle hypertrophy depend on muscle fiber types.

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“…Previous studies have reported on 5-week-old rats fed a high-fat diet (Bonilla et al, 2000;Kobayashi et al, 2014;Nomura et al, 2008) Therefore, the rats' age is not anticipated as a source of error in our experiment. While the final body weight of the WLE group was significantly lower than that of the high-fat diet group, WLE is not thought be associated with side effects or growth inhibition.…”

Section: Resultsmentioning

confidence: 89%

Anti-obesity Effects of Wasabi Leaf Extract on Rats Fed a High-fat Diet are Related to Upregulation of mRNA Expression of β3-adrenergic Receptors in Interscapular Brown Adipose Tissue

Yamada‐Kato¹,

Momoi

Okunishi³

et al. 2016

FSTR

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Wasabi is a plant of Japanese origin belonging to the Brassicaceae family. Although the wasabi rhizome is a popular condiment in Japan, the leaf is typically discarded. For utilization of the wasabi leaf, we investigated its antiobesity effect on Wistar rats fed a high-fat diet containing wasabi leaf extract (WLE) prepared with 50% ethanol.At the experimental endpoint, WLE had significantly decreased the body weight of rats and upregulated the mRNA expression of the β3-adrenergic receptor (Adrb3) in the interscapular brown adipose tissue (BAT). WLE may have promoted lipid absorption from the dietary fat, as the fecal lipid content was considerably lower in the WLE group. These results suggest that WLE suppressed obesity in rats fed a high-fat diet, which was related to the upregulation of Adrb3 mRNA expression in the interscapular BAT without increased fecal lipid excretion. Thus, wasabi leaves may be used as a functional food material for the suppression of obesity.

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Tea catechins enhance the mRNA expression of uncoupling protein 1 in rat brown adipose tissue

Cited by 75 publications

References 35 publications

Acute and Subacute Effects of Dexamethasone on the Number of White Blood Cells in Rats

Acute and Subacute Effects of Dexamethasone on the Number of White Blood Cells in Rats

Effects of the β2-Agonist Clenbuterol on β1- and β2-Adrenoceptor mRNA Expressions of Rat Skeletal and Left Ventricle Muscles

Anti-obesity Effects of Wasabi Leaf Extract on Rats Fed a High-fat Diet are Related to Upregulation of mRNA Expression of β3-adrenergic Receptors in Interscapular Brown Adipose Tissue

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