TDZ induces a dual morphogenetic pathway on leaf explants of the beach huckleberry – Gaylussacia brasiliensis

Direct Regeneration and Morpho-Histological Study of de novo Shoot Development from Leaf Explants of Rhododendron mucronulatum Turcz.

“…Thus, varying TDZ concentration can obtain totipotent or pluripotent cells. Moreover, in Gaylussacia brasiliensis leaf explants, the same concentrations of TDZ induced a dual morphogenesis pathway, when small protrusions on the adaxial leaf surface developed either into adventitious shoots or somatic embryos [15]. Thus, G. brasiliensis protrusion cells exhibited both pluri-and totipotency under the action of TDZ.…”

“…It was noted that TDZ-induced in vitro morphogenesis from Rhododendron leaf explants could occur through direct [10,11], indirect organogenesis [12,13] as well as somatic embryogenesis [14]. Moreover, it was reported that TDZ can simultaneously induce the development of somatic embryos and adventitious shoots on leaf explants of Gaylussacia brasiliensis from Ericaceae family [15]. It is well known that a variety of morpho-physiological anomalies in microshoots arise during TDZ-induced regeneration [16], therefore, it is often not possible to exactly identify the morphogenesis pathway when using morphological observations only [17].…”

Direct regeneration and morpho-histological study of de novo shoot development from leaf explants of Rhododendron mucronulatum Turcz.

“…From the result obtained it may be deduced that the TDZ depending on concentration is capable to make explant cells either totipotent or pluripotent. Moreover, in Gaylussacia brasiliensis leaf explants, the same concentrations of TDZ induced a dual morphogenesis pathway, when small protrusions on the adaxial leaf surface developed either into adventitious shoots or somatic embryos (Fritsche et al 2022). Thus, G. brasiliensis protrusion cells exhibited both pluri-and totipotency under the action of TDZ.…”

A four steps protocol for in vitro propagation of Eugenia uniflora L. (Myrtaceae)

“…Temperature rise has already shaped signi cant changes to natural systems, like extreme weather alterations, droughts, oods, and loss of biodiversity. In this context, tropical and subtropical forests are central ecosystems for the equilibrium of the climate and maintenance of the world's biological diversity (Stefenon et al 2020a, Fritsche et al 2022). However, climate change, habitat fragmentation, and pollinator failure directly impact the capacity of plant populations to reproduce effectively, generating direct consequences for their demography, evolution, and long-term persistence.…”

“…However, climate change, habitat fragmentation, and pollinator failure directly impact the capacity of plant populations to reproduce effectively, generating direct consequences for their demography, evolution, and long-term persistence. In this perspective, there is a growing consensus that underutilized crops may be important for the future production of food, as they tend to be more adapted to stressful conditions than domesticated crops (Fritsche et al 2022). The importance of Brazilian Myrtaceae species in this aspect is supported by the wide dispersion across the different biomes, including the species-rich Atlantic Forest and the grassland-dominated Pampa (Beise et al…”