TCR gene segments from at least one third of Va subfamilies rearrange at the δ locus

Genevée, Catherine; Chung, V.; Diu, Anita; Hercend, Thierry; Triebel, Frédéric

doi:10.1016/0161-5890(94)90083-3

Cited by 17 publications

(9 citation statements)

References 28 publications

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“…In one individual, these non-TCRDVI, non-TCRDV2 gene segments were used by the majority of 6 TCR transcripts. Including the data herein, members of at least 11 TCRAV gene families have now been shown to recombine with TCR 6 gene segments (13)(14)(15). These findings are consistent with those of others using immunohistochemistry and flow cytometry to report that y/6 cells in the small intestinal mucosa and colon express predominantly V61 or V62 (6,7,9).…”

Section: Discussionsupporting

confidence: 79%

“…We documented this herein for V62 and V63 transcripts, and it was known from prior studies (19,20) that V61 transcripts, which represent a minor component in peripheral blood, are oligoclonal. In addition, this may be the case for 6 TCR that use TCRAV (Va) gene segments (15). However, we note that the degree of oligoclonality of 6 transcripts in PBMC can vary among individuals, as exemplified by V62 transcripts from subjects PL and PJ.…”

Section: Discussionmentioning

confidence: 89%

“…The TCR 6 locus in humans maps within the TCR a locus on chromosome 14 (12) and several TCRAV (Va) gene segments are used by both TCR 6 and TCR a transcripts in the blood (13)(14)(15). Despite this shared use of V genes, the restriction elements and ligands recognized by y/6 T cells appear to differ from those recognized by a/f T cells, emphasizing the likely importance of junctional regions that encode the complementarity determining region (CDR) 3 for ligand recognition ( 16).…”

Section: Introductionmentioning

confidence: 99%

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The delta T cell receptor repertoire in human colon and peripheral blood is oligoclonal irrespective of V region usage.

Holtmeier

Chowers²,

Lumeng³

et al. 1995

J. Clin. Invest.

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The majority of y/8 T cell receptors (TCR) in the humanIntestinal mucosa are thought to use the TCRDV1 (V81) variable region gene segment, whereas y/8 T cells in the circulation predominantly express the TCRDV2 (V82) gene segment. 8 T cell receptors that use the TCRDV2 variable region gene segment generally have been regarded as highly diverse, whereas those that use the TCRDV1 gene segment are oligoclonal, whether present in the intestinal tract or in peripheral blood. We report herein that oligoclonality is a genal feature of the peripheral 8 T cell receptor repertoire In heWthy human adults, irrespective of the variable region used and regardless of whether y/8 T cells reside in the intestinal mucosa or in peripheral blood. In addition, the 8 T cell receptor repertoire is shown to be highly compartmentalized between such sites as the colon and peripheral blood, relatively stable over at least a 10-16-mo period, and unique in each individual. Further, the spectrum of variable region genes used by 8 T cell receptor transcripts in the human c.Ia is greater than previously recognized. Thus, in additin to the TCRDV1 and TCRDV2 variable region gene segments, 8 T cell receptors in normal intestinal mucosa can use TCRDV3 (V83) and TCRAV (Va) gene segments which, in some individuals, comprise a significant component of the mucosal 8 T cell receptor repertoire. Our studies indicate that the potential of 8 T cell receptors for extensive diversity is not reflected in the mature human repertoire. Moreover, these findings suggest a model wherein the 8 T cell receptor repertoire in the colon and peripheral blood is aped by selection and clonal expansion of y/v T cells that thuwtely seed throughout the length of the colon mucosa and populate the circulation. (J.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The delta T cell receptor repertoire in human colon and peripheral blood is oligoclonal irrespective of V region usage.

Holtmeier

Chowers²,

Lumeng³

et al. 1995

J. Clin. Invest.

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“…Concerning the remarkable finding of two V␣-J␦1 expressing T-ALL, it should be noted that normal human ␥␦ + T cells using a V␣ gene segment in their TCR␦ chain have also been described. 37,38 Nevertheless, the vast majority of TCR␥␦ + T-ALL in our study expressed V␦1-J␦1-C␦ chains, disulphide-linked to either V␥I-J␥2.3-C␥2 or V␥II-J␥2.3-C␥2 chains. Analysis of V␥ protein expression revealed that V␥I(V␥2,3,4,5,8) expression was dominant over V␥II(V␥9) expression.…”

Section: Tablementioning

confidence: 61%

Immunophenotypic and immunogenotypic characteristics of TCRγδ+ T cell acute lymphoblastic leukemia

et al. 1999

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“…This decrease of nibbling is probably affected by N addition through the increased expression of hTdTS in adult, as discussed above. The persistent expression of hTdTS and hTdTL2 in all stages of thymocyte development also explains the presence of N addition and nucleotide deletion in human TCR-␤, -␣, -␥, and -␦ chain genes (51)(52)(53)(54)(55).…”

Section: Discussionmentioning

confidence: 99%

Distinct and Opposite Activities of Human Terminal Deoxynucleotidyltransferase Splice Variants

Thai

Kearney

2004

The Journal of Immunology

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Evidence for potential human TdT (hTdT) isoforms derived from hTdT genomic sequences led us to identify the short isoform (hTdTS), as well as mature long transcripts containing exon XII (hTdTL1) and another including exon VII (hTdTL2) in lymphoid cells. Normal B and T lymphocytes express exclusively hTdTS and hTdTL2, whereas hTdTL1 expression appears to be restricted to transformed lymphoid cell lines. In in vitro recombination and primer assays, both long isoforms were shown to have 3′→5′ exonuclease activity. Overexpression of hTdTS or hTdTL2 greatly reduced the efficiency of recombination, which was reverted to normal levels by the simultaneous expression of both enzymes. Therefore, alternative splicing may prevent the adverse effects of unchecked elongation or diminution of coding ends during V(D)J recombination, thus affecting the survival of a B or T cell precursor during receptor gene rearrangements. Finally, the newly discovered hTdT isoforms should be considered in future screening of human leukemias.

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TCR gene segments from at least one third of Va subfamilies rearrange at the δ locus

Cited by 17 publications

References 28 publications

The delta T cell receptor repertoire in human colon and peripheral blood is oligoclonal irrespective of V region usage.

The delta T cell receptor repertoire in human colon and peripheral blood is oligoclonal irrespective of V region usage.

Immunophenotypic and immunogenotypic characteristics of TCRγδ+ T cell acute lymphoblastic leukemia

Distinct and Opposite Activities of Human Terminal Deoxynucleotidyltransferase Splice Variants

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