Immunophenotypic and immunogenotypic characteristics of TCRγδ+ T cell acute lymphoblastic leukemia

Abstract

Thirty T cell receptor (TCR)␥␦Firstly, more complete TCRD rearrangements were identified on the non-expressed allele in the non-V␦1 + group (83% vs 43%); secondly, a higher frequency of 'end-stage' J␥2.3 gene rearrangements was found in non-V␦1 cases on both TCRG alleles (83% vs 66%); thirdly, a higher frequency of complete TCRB rearrangements was found in non-V␦1 cases (79% vs 50%).

Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: Report of the BIOMED-2 Concerted Action BMH4-CT98-3936

“…[11][12][13][14][15][16] In the interpretation of monotypic Vb results using 20-25 antibodies against different Vb families (Table 2), one should realize that 1 Jaffe et al, 2 and van Dongen et al 5 …”

“…It should be noted that most TCRgd þ T-cell malignancies express Vd1 or another non-Vd2 gene segment in combination with a single Vg domain (generally not Vg9). 15,20 The detection of Igk-or Igl-restricted expression or monotypic Vb, Vg or Vd expression is relatively easy in flow cytometric studies of PB and bone marrow (BM) samples of patients with mature B-or T-cell leukemias. However, it appears to be more difficult in tissue samples with suspect lymphoproliferative disorders that are intermixed with normal (reactive) lymphocytes.…”

“…33,34 Virtually all (498%) TCRab þ T-cell malignancies have TCRG gene rearrangements (generally biallelic) and many TCRgd þ Tcell malignancies have TCRB gene rearrangements, implying that the detection of TCRB or TCRG rearrangements is not indicative of T cells of the ab or gd T-cell lineage, respectively, either. 15,27,28 In addition to these crosslineage rearrangements, it has been established that several lymphoid malignancies have unusual Ig/ TCR gene rearrangement patterns. This information is available in detail for precursor B-ALL and T-ALL, but not yet for most other lymphoid malignancies.…”

Cross-lineage T cell receptor gene rearrangements occur in more than ninety percent of childhood precursor-B acute lymphoblastic leukemias: alternative PCR targets for detection of minimal residual disease

“…2 Incomplete and complete TCRB gene rearrangements were detected with TCRBD1U, TCRBD1, TCRBJ1, TCRBD2U, TCRBD2, TCRBJ2 and TCRBC probes (Ref. 47; DAKO Corporation, Carpinteria, CA, USA) in EcoRI and HindIII digests. TCRG gene rearrangements were analyzed with the J␥1.2 probe in BglII digests and the J␥1.3 and J␥2.1 probes in EcoRI digests.…”

“…This is most probably owing to the usage of the four different D␤ probes, which allow identification of incomplete V-D␤, D-D␤, and D-J␤ gene rearrangements. 47 Four other research groups analyzed and compared all three cross-lineage TCR genes in a total number of 100 cases. 5,7,10,11 Combining these published data and the data presented here show that TCRD gene rearrangements and/or deletions occurred in the majority of cases (ෂ90%) and that 27% of childhood precursor-B-ALL had TCRD gene rearrangements and/or deletions with germline TCRG and TCRB genes (Table 5).…”