TbtABM, a multidrug efflux pump associated with tributyltin resistance in<i>Pseudomonas stutzeri</i>

Jude, F.; Arpin, Corinne; Brachet-Castang, C; Capdepuy, M.; Cartigny, Pierre; Quentin, Claudine

doi:10.1016/s0378-1097(04)00012-6

Cited by 54 publications

(42 citation statements)

References 36 publications

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“…Bacterial populations could be useful in detoxifying these agents. Biocides that are degraded and/or resisted by P. stutzeri strains are tributyltin (167,168,299), an organostannic compound used industrially as a stabilizer in plastics and wood preservatives and as an antifouling agent in boat paints; nonoxidizing industrial water treatment bactericides (52)(53)(54)194), used in industrial water cooling systems to avoid microbially induced corrosion of metal surfaces; ␤-cyfluthrin (304), a pesticide used in agriculture to control lepidopteran pests affecting solanaceous crops; and cyanide and thiocyanates (129,174,(389)(390)(391), used in petrochemical refining, the synthesis of organic chemicals and plastics, electroplating, aluminum works, and metal mining.…”

Section: Biodegradation and Useful Properties For Biotechnological Apmentioning

confidence: 99%

“…Tributyltin resistance was found to be associated with the presence of the operon tbtABM. It is a member of the resistance-nodulation-cell division efflux pump family (168). Interestingly, TbtABM conferred a multidrug resistance phenotype to strain 5MP1, including resistance to n-hexane, nalidixic acid, chloramphenicol, and sulfamethoxazole (168).…”

Section: Biodegradation and Useful Properties For Biotechnological Apmentioning

confidence: 99%

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Biology of Pseudomonas stutzeri

Lalucat

Bennasar

Bosch

et al. 2006

Microbiol Mol Biol Rev

530

418

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SUMMARY Pseudomonas stutzeri is a nonfluorescent denitrifying bacterium widely distributed in the environment, and it has also been isolated as an opportunistic pathogen from humans. Over the past 15 years, much progress has been made in elucidating the taxonomy of this diverse taxonomical group, demonstrating the clonality of its populations. The species has received much attention because of its particular metabolic properties: it has been proposed as a model organism for denitrification studies; many strains have natural transformation properties, making it relevant for study of the transfer of genes in the environment; several strains are able to fix dinitrogen; and others participate in the degradation of pollutants or interact with toxic metals. This review considers the history of the discovery, nomenclatural changes, and early studies, together with the relevant biological and ecological properties, of P. stutzeri.

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Section: Biodegradation and Useful Properties For Biotechnological Apmentioning

confidence: 99%

Section: Biodegradation and Useful Properties For Biotechnological Apmentioning

confidence: 99%

Biology of Pseudomonas stutzeri

Lalucat

Bennasar

Bosch

et al. 2006

Microbiol Mol Biol Rev

530

418

View full text Add to dashboard Cite

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“…Despite their significance as determinants of antibiotic resistance, however, RND-type multidrug exporters also, in many instances, accommodate biocides (42,45), organic solvents (48), detergents (43), including bile salts (9,18,46,60), toxic fatty acids/lipids (54), and in some instances, plant-derived antimicrobials (phytoalexins and isoflavonoids) (7,39), metabolic inhibitors (52), organometallic compounds (tributyltin) (25), quorum-sensing effector molecules (13,26,40), and, possibly, virulence factors (21) in addition to antibiotics. Clearly, RND pumps can and do function as other than antibiotic exporters.…”

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confidence: 99%

Antibiotic Inducibility of the MexXY Multidrug Efflux System of Pseudomonas aeruginosa : Involvement of the Antibiotic-Inducible PA5471 Gene Product

2006

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The MexXY components of the MexXY-OprM multidrug efflux system of Pseudomonas aeruginosa are encoded by a MexZ repressor-regulated operon that is inducible by antibiotics that target the ribosome. Mutant strains disrupted in a gene, PA5471, were shown to be compromised for drug-inducible mexXY expression and, therefore, MexXY-OprM-mediated antimicrobial resistance. The PA5471 gene was inducible by the same ribosometargeting agents that induce mexXY expression. Moreover, vector-driven expression of cloned PA5471 was sufficient to promote mexXY expression and MexXY-mediated resistance in the absence of antibiotic exposure, consistent with PA5471 directly or indirectly activating mexXY expression following its own upregulation in response to antibiotics. The requirement for PA5471 for mexXY expression and antimicrobial resistance was, however, obviated in mutants lacking the MexZ repressor of mexXY expression, suggesting that PA5471 directly or indirectly modulates MexZ activity in effecting mexXY expression. While the recruitment of PA5471 and MexXY in response to ribosome disruption by antimicrobials is consistent with their genes playing a role in protecting cells from the adverse consequences of disrupting the translation process, reminiscent of transtranslation, these genes appear to operate independently in their contribution to resistance: mutants defective in trans-translation showed a much more modest (twofold) decrease in resistance to ribosome-targeting agents than those lacking PA5471 or MexXY, and this decrease was observed whether functional PA5471/MexXY was present or not.Multidrug efflux systems of the resistance-nodulation-division (RND) family contribute significantly to intrinsic and acquired resistance to antimicrobials in a number of gram-negative bacteria (43, 45). Despite their significance as determinants of antibiotic resistance, however, RND-type multidrug exporters also, in many instances, accommodate biocides (42, 45), organic solvents (48), detergents (43), including bile salts (9,18,46,60), toxic fatty acids/lipids (54), and in some instances, plant-derived antimicrobials (phytoalexins and isoflavonoids) (7, 39), metabolic inhibitors (52), organometallic compounds (tributyltin) (25), quorum-sensing effector molecules (13,26,40), and, possibly, virulence factors (21) in addition to antibiotics. Clearly, RND pumps can and do function as other than antibiotic exporters.Pseudomonas aeruginosa expresses several three-component RND-type multidrug efflux systems, among which four, MexAB-

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“…In contrast, AcrAB exported tributyltin much more efficiently than AheABC did, although this antifouling agent is a common pollutant of water sediments that can occasionally contaminate the Aeromonas sp. ecosystem (12).…”

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confidence: 99%