Biology of Pseudomonas stutzeri

Abstract

SUMMARY Pseudomonas stutzeri is a nonfluorescent denitrifying bacterium widely distributed in the environment, and it has also been isolated as an opportunistic pathogen from humans. Over the past 15 years, much progress has been made in elucidating the taxonomy of this diverse taxonomical group, demonstrating the clonality of its populations. The species has received much attention because of its particular metabolic properties: it has been proposed as a model organism for denitrification studies; many strains have natural transformation properties, making it relevant for study of the transfer of genes in the environment; several strains are able to fix dinitrogen; and others participate in the degradation of pollutants or interact with toxic metals. This review considers the history of the discovery, nomenclatural changes, and early studies, together with the relevant biological and ecological properties, of P. stutzeri.

Segregating metabolic processes into different microbial cells accelerates the consumption of inhibitory substrates

“…Second, we can manipulate the growth-inhibiting effects of the cross-fed metabolic intermediatenitrite-and measure the consequences on substrate consumption. Nitrite accumulates within the periplasm during denitrification (Lalucat et al, 2006;Klueglein et al, 2014) and can inhibit growth by at least two different pH-dependent mechanisms, both of which act indirectly. First, as the pH decreases, nitrite increasingly protonates to nitrous acid (HNO 2 ) and uncouples proton translocation (Sijbesma et al, 1996;Zhou et al, 2011).…”

“…To accomplish this, we genetically engineered a novel substrate cross-feeding system based on the denitrification pathway of the Gram-negative bacterium Pseudomonas stutzeri A1501 (Yan et al, 2008). In the absence of oxygen, P. stutzeri can support its growth by sequentially reducing nitrate (NO 3 − ) to nitrite (NO 2 − ), nitric oxide (NO), nitrous oxide (N 2 O) and finally to dinitrogen gas (N 2 ), where the nitrogen oxides serve as terminal electron acceptors (Lalucat et al, 2006). We constructed three isogenic mutant strains of P. stutzeri A1501 that differ in their ability to reduce nitrate and nitrite (note that asparagine is supplied in excess as the nitrogen source for biosynthesis; Figure 2 and Supplementary Table 1).…”

Interaction between NifL and NifA in the nitrogen-fixing Pseudomonas stutzeri A1501

“…The nitrogen fixation ability within the genus Pseudomonas has been questioned for a long time (for reviews see Chan et al, 1994;Lalucat et al, 2006). It is now established that several strains of Pseudomonas stutzeri can fix nitrogen (Vermeiren et al, 1999;Rediers et al, 2004).…”

Nitrogen fixation island and rhizosphere competence traits in the genome of root-associated Pseudomonas stutzeri A1501

“…The taxonomic status and biology of this species, isolated from a large diversity of terrestrial and marine environments, have been recently reviewed (6). Some strains have attracted particular attention because of specific metabolic properties, such as denitrification, degradation of aromatic compounds, and synthesis of polyhydroxyalkanoates (6). A1501 (China General Microbiological Culture Collection Center accession no.…”