Taxonomy of Psychrophiles

Nogi, Yuichi

doi:10.1007/978-4-431-53898-1_38

Cited by 11 publications

(9 citation statements)

References 97 publications

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“…Up to now, most PUFA‐producing bacteria belong to the class of Gammaproteobacteria such as Shewanella and Psychromonas (Fang et al., 2004; Freese et al., 2008; Nogi et al., 2007). Another group of marine bacteria that produce PUFAs was found within the Bacteroidetes such as Flavobacterium , Flexibacter and Psychroserpens (Nogi, 2011). In the present study, adult S. intermedius fed kelp showed the highest level of LC‐PUFA but the lowest abundance of Psychromonas among the known PUFA‐producing bacteria.…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary n‐3 LC‐PUFA on the growth performance, gonad development, fatty acid profile, transcription of related genes and intestinal microflora in adult sea urchin ( Strongylocentrotus intermedius )

Zhang

Ding

et al. 2020

Aquaculture Research

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A study was conducted to investigate the effects of dietary n‐3 LC‐PUFA on the growth performance, fatty acid profile, transcription of related genes and intestinal microflora in adult sea urchin (Strongylocentrotus intermedius). Kelp (Laminaria japonica) and four formulated feeds with graded levels of n‐3 LC‐PUFA (0%, 0.5%, 0.8% and 1.0% dry diet) were prepared as the experimental diets. Each diet was randomly allocated to ten adult sea urchin individuals. The feeding period lasted for 90 days. Results showed that gonadosomatic index (GSI) of adult S. intermedius fed formulated feeds was significantly higher than that fed kelp (p < 0.05), while weight gain rate (WGR) showed no significance between kelp and feed groups (p > 0.05). Neither WGR nor GSI showed significant differences among the four feed treatments (p > 0.05). Notably, ARA and EPA in the gonads of S. intermedius fed the feeds devoid of n‐3 LC‐PUFA were comparable to that fed the feeds with 0.5% n‐3 LC‐PUFA (p > 0.05). Transcription of Elovl4 and Δ5‐desaturase in the intestine and gonad significantly decreased as dietary n‐3 LC‐PUFA increased (p < 0.05). Proteobacteria and Bacteroidetes (phylum level) and Psychromonas and Sulfurimonas (genus level) were the most abundant bacteria species in the intestine of S. intermedius. The abundance of Sulfurimonas was comparable in the S. intermedius fed kelp and the feed with 0% n‐3 LC‐PUFA, which was significantly higher than that fed the other formulated feeds (p < 0.05). These results indicated that S. intermedius seems to have a low requirement for n‐3 LC‐PUFA to sustain its normal growth and gonad development. The increased transcription of rate‐limiting enzymes (Elovl4 and Δ5‐desaturase) could be responsible for the considerable amounts of EPA and ARA in the gonads of S. intermedius when fed feeds without LC‐PUFA. The intestinal microflora were affected to a certain extent by the die categories and the n‐3 LC‐PUFA in the formulated feeds. It is speculated that some unknown bacteria in the genus of Sulfurimonas could participate in the synthesis of LC‐PUFA.

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Section: Discussionmentioning

confidence: 99%

Effects of dietary n‐3 LC‐PUFA on the growth performance, gonad development, fatty acid profile, transcription of related genes and intestinal microflora in adult sea urchin ( Strongylocentrotus intermedius )

Zhang

Ding

et al. 2020

Aquaculture Research

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“…The diversity of psychrophilic bacteria comprises genera as Pseudomonas , Psychrobacter, Pseudoalteromonas , Colwellia , Arthrobacter , etc. [ 48 ].…”

Section: Extreme Environments and Extremophilesmentioning

confidence: 99%

Plastic Degradation by Extremophilic Bacteria

Atanasova

Stoitsova

Paunova-Krasteva

et al. 2021

IJMS

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Intensive exploitation, poor recycling, low repeatable use, and unusual resistance of plastics to environmental and microbiological action result in accumulation of huge waste amounts in terrestrial and marine environments, causing enormous hazard for human and animal life. In the last decades, much scientific interest has been focused on plastic biodegradation. Due to the comparatively short evolutionary period of their appearance in nature, sufficiently effective enzymes for their biodegradation are not available. Plastics are designed for use in conditions typical for human activity, and their physicochemical properties roughly change at extreme environmental parameters like low temperatures, salt, or low or high pH that are typical for the life of extremophilic microorganisms and the activity of their enzymes. This review represents a first attempt to summarize the extraordinarily limited information on biodegradation of conventional synthetic plastics by thermophilic, alkaliphilic, halophilic, and psychrophilic bacteria in natural environments and laboratory conditions. Most of the available data was reported in the last several years and concerns moderate extremophiles. Two main questions are highlighted in it: which extremophilic bacteria and their enzymes are reported to be involved in the degradation of different synthetic plastics, and what could be the impact of extremophiles in future technologies for resolving of pollution problems.

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“…Interestingly, most enzymes originating from deep-sea organisms remain active at high pressure, while related enzymes from land organisms lose their activity (Kato et al, 2008). For example, 3-isopropylmalate dehydrogenase (IPMDH) from the obligate piezophile Shewanella benthica DB21MT-2, originally isolated from the Mariana Trench (Kato et al, 1998;Nogi & Kato, 1999), was shown to be more tolerant to highpressure stress than the same enzyme originating from the nonpiezophile S. oneidensis MR-1 (Kasahara et al, 2009). IPMDH catalyzes the reduction of 3-isopropylmalate (IPM) to 2-isopropyl-3-oxosuccinate in the presence of divalent metal cations such as magnesium or manganese ion and nicotinamide adenine dinucleotide.…”

Section: Introductionmentioning

confidence: 99%

High-pressure-induced water penetration into 3-isopropylmalate dehydrogenase

Nagae

Kawamura

Chavas

et al. 2012

Acta Crystallogr D Biol Cryst

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Hydrostatic pressure induces structural changes in proteins, including denaturation, the mechanism of which has been attributed to water penetration into the protein interior. In this study, structures of 3-isopropylmalate dehydrogenase (IPMDH) from Shewanella oneidensis MR-1 were determined at about 2 Å resolution under pressures ranging from 0.1 to 650 MPa using a diamond anvil cell (DAC). Although most of the protein cavities are monotonically compressed as the pressure increases, the volume of one particular cavity at the dimer interface increases at pressures over 340 MPa. In parallel with this volume increase, water penetration into the cavity could be observed at pressures over 410 MPa. In addition, the generation of a new cleft on the molecular surface accompanied by water penetration could also be observed at pressures over 580 MPa. These water-penetration phenomena are considered to be initial steps in the pressuredenaturation process of IPMDH.

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Taxonomy of Psychrophiles

Cited by 11 publications

References 97 publications

Effects of dietary n‐3 LC‐PUFA on the growth performance, gonad development, fatty acid profile, transcription of related genes and intestinal microflora in adult sea urchin ( Strongylocentrotus intermedius )

Effects of dietary n‐3 LC‐PUFA on the growth performance, gonad development, fatty acid profile, transcription of related genes and intestinal microflora in adult sea urchin ( Strongylocentrotus intermedius )

Plastic Degradation by Extremophilic Bacteria

High-pressure-induced water penetration into 3-isopropylmalate dehydrogenase

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