Taxonomy and multi-locus phylogeny of cylindrocarpon-like species associated with diseased roots of grapevine and other fruit and nut crops in California

Lawrence, Daniel P.; Nouri, M. T.; Trouillas, Florent P.

doi:10.3114/fuse.2019.04.06

Cited by 29 publications

(41 citation statements)

References 42 publications

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“…With the four sets of primers used, we have achieved our goal of showing the spectrum of different species involved in the development of RRD symptoms, even in a single plant. Similar to our studies, Lawrence et al 67 identified, in a multi-locus sequence typing of four loci (ITS, TUB2, TEF1, and HIS), 12 Cylindrocarpon -like fungal species across five genera associated with black foot disease of grapevine and other diverse root diseases of fruit and nut crops in California. They stated that the HIS marker was essential, either singly or in combination with the other mentioned genes, for the precise identification of most Dactylonectria species.…”

Section: Discussionsupporting

confidence: 88%

“…were also determined to be main causative pathogens of Rosaceous small fruit root diseases 17 and of the decline and root rot of loquat ( Eriobotrya japonica ‘Algerie’) 15 . Furthermore, Cylindrocarpon -like species have been described in connection with root rot and decline effects in kiwifruit ( Actinidia chienensis ), avocado ( Persea americana ), olive ( Olea europaea ), walnut ( Juglans regia ), and grapevine ( Vitis vinifera ) 67 . This underlines the wide host spectrum of these pathogenic Nectriaceae, which goes beyond the Rosaceae.…”

Section: Discussionmentioning

confidence: 99%

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Observations on early fungal infections with relevance for replant disease in fine roots of the rose rootstock Rosa corymbifera 'Laxa'

Grunewaldt-Stöcker

Popp

Baumann

et al. 2020

Sci Rep

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Replant disease is a worldwide phenomenon affecting various woody plant genera and species, especially within the Rosaceae. Compared to decades of intensive studies regarding replant disease of apple (ARD), the replant disease of roses (RRD) has hardly been investigated. The etiology of RRD is also still unclear and a remedy desperately needed. In greenhouse pot trials with seedlings of the RRD-sensitive rootstock Rosa corymbifera ‘Laxa’ cultured in replant disease affected soils from two different locations, early RRD symptom development was studied in fine roots. In microscopic analyses we found similarities to ARD symptoms with regards to structural damages, impairment in the root hair status, and necroses and blackening in the cortex tissue. Examinations of both whole mounts and thin sections of fine root segments revealed frequent conspicuous fungal infections in association with the cellular disorders. Particularly striking were fungal intracellular structures with pathogenic characteristics that are described for the first time. Isolated fungi from these tissue areas were identified by means of ITS primers, and many of them were members of the Nectriaceae. In a next step, 35 of these isolates were subjected to a multi-locus sequence analysis and the results revealed that several genera and species were involved in the development of RRD within a single rose plant. Inoculations with selected single isolates (Rugonectria rugulosa and Ilyonectria robusta) in a Perlite assay confirmed their pathogenic relationship to early necrotic host plant reactions, and symptoms were similar to those exhibited in ARD.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Observations on early fungal infections with relevance for replant disease in fine roots of the rose rootstock Rosa corymbifera 'Laxa'

Grunewaldt-Stöcker

Popp

Baumann

et al. 2020

Sci Rep

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“…Many novel Ilyonectria and Ilyonectria-like species have been discovered recently (Carlucci et al, 2017;Lawrence et al, 2019). Due to the slow growth of some fungi, it is not easy to obtain species using separation techniques.…”

Section: Discussionmentioning

confidence: 99%

Multi-Locus Phylogeny and Taxonomy of the Fungal Complex Associated With Rusty Root Rot of Panax ginseng in China

et al. 2020

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Panax ginseng rusty root rot caused by the Ilyonectria species complex is a devastating disease, and it is one of the main factors contributing to the difficulty in continual cropping. Rusty root rot occurs in all ginseng fields, but little is known about the taxonomy of the fungal pathogen complex, especially Ilyonectria and Ilyonectria-like species. Rusty root rot samples were collected from commercial ginseng cultivation areas of China, and the pathogens were isolated and purified as single spores. Based on the combination analysis of multiple loci (rDNA-ITS, TUB, HIS3, TEF, ACT, LSU, RPB1, RPB2, and SSU) and morphological characteristics, the pathogens causing ginseng rusty root rot were determined. Fungal isolates were obtained from infected roots in 56 locations within main cultivation areas in China. A total of 766 strains were identified as Ilyonectria, Ilyonectria-like and Rhexocercosporidium species, including I. robusta (55.0%), I. communis (21.7%), I. mors-panacis (10.9%), I. pseudodestructans (2.0%), I. changbaiensis (1.3%), I. qitaiheensis (1.3%), Neonectria obtusispora (2.0%), Dactylonectria torresensis (0.5%), D. sp. (0.5%), and R. panacis (1.5%), and four novel species, Thelonectria ginsengicola (1.0%), T. jixiensis (1.0%), T. mulanensis (0.8%) and T. fusongensis (0.5%), with a total of 14 species. As the pathogen present in the highest proportion, I. robusta was the most prevalent and damaging species, unlike the pathogens reported previously. All of the examined strains were proven to cause ginseng rusty root rot. Our results indicate that the taxonomy of the fungal complex associated with ginseng rusty root rot includes Ilyonectria, Ilyonectria-like genera (Dactylonectria, Neonectria, and Thelonectria) and Rhexocercosporidium.

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“…Up to 32 species of the genera Campylocarpon , Cylindrocladiella , Dactylonectria , Ilyonectria , Neonectria , Pleiocarpon and Thelonectria have been reported to cause black‐foot disease, 1,6–8 Dactyonectria torresensis is the most prevalent species associated with diseased vines in Europe 9–11 . These fungal species are known to be soilborne and persist as mycelium and conidia in rotten root fragments or as resting spores (chlamydospores) that can survive in the soil for extended periods of time after infected plants are removed 4,12 .…”

Section: Introductionmentioning

confidence: 99%

Field evaluation of biocontrol agents against black‐foot and Petri diseases of grapevine

Martínez-Diz

Losada

Andrés-Sodupe

et al. 2020

Pest Management Science

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BACKGROUND Black‐foot and Petri diseases are the main fungal diseases associated with young grapevine decline. Two field experiments were established to evaluate the preventive effect of two potential biocontrol agents (BCAs), that is Streptomyces sp. E1 + R4 and Pythium oligandrum Po37, and three BCA‐commercial products containing Trichoderma atroviride SC1, Trichoderma koningii TK7 and Pseudomonas fluorescens + Bacillus atrophaeus on fungal infection in grafted plants and plant growth parameters. RESULTS The effectiveness of some BCA in reducing the incidence and severity of both diseases was dependent on the plant part analyzed and the plant age. No single BCA application was able to control both diseases. Streptomyces sp. E1 + R4 were able to reduce significantly the infection of the most prevalent black‐foot disease fungi while P. oligandrum Po37 and Trichoderma spp. were able to reduce significantly Phaeomoniella chlamydospora and Phaeoacremonium minimum (Petri disease) infection. BCA treatments had no effect on the shoot weight, and root weight was significantly lower in all BCA treatments with respect to the control. CONCLUSIONS The combination of the disease‐suppressive activity of two or more beneficial microbes in a biocontrol preparation is required to prevent infection by black‐foot and Petri disease fungi in vineyards.

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Taxonomy and multi-locus phylogeny of cylindrocarpon-like species associated with diseased roots of grapevine and other fruit and nut crops in California

Cited by 29 publications

References 42 publications

Observations on early fungal infections with relevance for replant disease in fine roots of the rose rootstock Rosa corymbifera 'Laxa'

Observations on early fungal infections with relevance for replant disease in fine roots of the rose rootstock Rosa corymbifera 'Laxa'

Multi-Locus Phylogeny and Taxonomy of the Fungal Complex Associated With Rusty Root Rot of Panax ginseng in China

Field evaluation of biocontrol agents against black‐foot and Petri diseases of grapevine

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