Taxonomic Study of Extreme Halophilic Archaea Isolated from the “Salar de Atacama”, Chile

Lizama, Catherine; Monteoliva‐Sánchez, Mercedes; Prado, Bernardo; Ramos‐Cormenzana, A.; Weckesser, Jürgen; Campos, Victoriano

doi:10.1078/0723-2020-00053

Cited by 41 publications

(24 citation statements)

References 37 publications

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“…At present, thes e enzymes represent about 20% of commercialized enzymes in industry (López-López et al, 2015), as they have great potential in several industrial processes such as production of biodegradable polymers, detergents, food flavoring, oil biodegradation, or waste treatment (Anobom et al, 2014). In this study, 25.9% of the screened strains displayed lipase activity, this percentage was similar to previous study of Lizama et al (2001) who stated that the 20% of isolated haloarchaeal strains from Salt Lake in Chile produced amylase and lipase. Moreover, protease enzyme was the most common enzyme of screened strains (represented 39.4%), and produced by species with various genera, Halobiforma, Natronococcus, Natronobacterium , Natrinema, Natrialba, Natronomonas and Haloterrigena.…”

Section: Discussionsupporting

confidence: 79%

Screening of Hydrolytic Extremozymes in Haloalkaliphilic Archaea by Culture and Molecular-based Methods

Hamedo¹,

Hagagy²,

Shafi³

et al. 2017

Egypt. J. Exp. Biol. (Bot.)

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Screening of hy droly tic extremoz y mes in haloalkali ph ilic Archaea by culture and molec ular -bas ed m ethods ABSTRACT:Exploring of extrem ophilic Archaea and their enzym es had great significance to biocatalysis. Enzym es produced by Archaea allow im provem ent in m ultiple sectors of industry. They can help reduce the quantity of w aste energy and material consumption, thus m aking the technology m ore environm entally-friendly. This study aim ed to screen hydrolytic extrem ozym es in different Soda Lakes of W adi Al-Natrun, Egypt, by enzym atic agar -plate assays and m olecular-based m ethods. Five hundred and thirty-five haloalkaliphilic archaeal strains isolated from different Soda Lakes w ere screened for production of protease, am ylase, pectinase, chitinase, cellulase, lipase and esterase at pH 10 and 25% NaCl (w /v). Furtherm ore, m etagenomic DNA w as extracted from w ater sam ple of Ga'ar Lake and constructed library w ere sequenced to identify the genes encoding target enzymes by using illum ina Hiseq2000 system . By enzym atic agarplate assay, all tested strains show ed potential production of extracellular enzym es, a total of 39.4% of screened strains produced protease, 27.1% show ed am ylase activity, 25.9% for lipase and 7.4% displayed cellulase activity, but none of tested strains produced chitinase or pectinase. W hile, by shotgun m etagenomic technique, all genes encoding m etabolically active hydrolytic enzym es studied w er e detected in water sam ple of Ga'ar Soda Lake. Metagenome-derived DNA libraries have focused on m any classes of enzym es, am ong these hydrolytic enzym es were prominent. The results of both m ethods indicated that these soda lakes are rich w ith commercially valuable enzym es.

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Section: Discussionsupporting

confidence: 79%

Screening of Hydrolytic Extremozymes in Haloalkaliphilic Archaea by Culture and Molecular-based Methods

Hamedo¹,

Hagagy²,

Shafi³

et al. 2017

Egypt. J. Exp. Biol. (Bot.)

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“…High salinities and organic compounds in isolation media can act as a selective factor to isolate members of this genus. Although the microbial diversity of hypersaline environments is of great interest during last decade, only a few studies are conducted that concern the production of extracellular enzymes from halo archaea as predominant microorganisms of this extreme environment [30]. The ability to produce 4 hydrolytic enzymes was assayed qualitatively among selected strains of IncheBroun wetland.…”

Section: Volume 56mentioning

confidence: 99%

Isolation, Identification and Extracellular Enzymatic Activity of Culturable Extremely Halophilic Archaea and Bacteria of IncheBoroun Wetland

Rasooli

Amoozegar

Sepahy

et al. 2016

ILNS

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Abstract. Extremely halophilic diversity of IncheBroun wetland located in the north of Iran was investigated by using culture-dependent methods. Sampling was carried out in May and September 2014. In each sampling 4 distinct regions of wetland were analyzed by using complex media like MGM, JCM168, MH1 and an alkaliphilic medium containing 23% salts. After incubation at 40˚C, a total of 406 isolates and 2.1 × 10 6 CFU/ml were obtained in culture media. Among them 361 isolates were obtained from MGM and 39 isolates from JCM 168, 3 isolates from MH1 and 3 isolates from the alkaliphilic media. Initial morphological, biochemical and physiological tests were performed. Production of 4 hydrolytic enzymes by 45 selected strains was assayed qualitatively. A total of 38, 19 and 6 strains were able to produce lipase, DNase and amylase activity. Protease activity was not observed among strains. As total 45 strains were selected randomly and phylogenetic analysis of 16S rRNA was performed for them. Among selected strains 40 isolated strians belonged to Haloarchaea and were belonged to the genera: Haloarcula(30%), Halorubrum(27.5%), Haloferax(17.5%), Halobellus (10%), Halogeometricum(5.2%), Halobacterium(2.6%), Halolamina(2.6%), Halorhabdus (2.6%) and Halostagnicola (2.6%). Haloarcula and Halorubrum were the dominant populations. A total of 5 strains belonged to domain of Bacteria and were similar to members of Rhodovibrio (40%), Pseudomonas (40%) and Salicola (20%).

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“…Knowledge of the biodiversity in salty environments opens up a possibility to identify novel microorganisms and to understand the functioning of their ecosystems [1]. Additionally, microbial research in saline environments is important for multiple reasons: halophilic microorganisms produce unusual compounds which could be a basis for novel biotechnological applications; terrestrial saline environments may shed light on life on Mars, as this planet is rich in salt deposits and saline environments; primordial life on earth might have started in hypersaline environments [2].…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic analysis of the bacterial community in a crystallizer pond, Pomorie salterns, Bulgaria

Kambourova

Tomova

Boyadzhieva

et al. 2016

Biotechnology & Biotechnological Equipment

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The aim of this study was to investigate the bacterial community habituating P18, the biggest crystallizer pond in Pomorie salterns (34% salinity). The obtained results showed that the bacterial community differs from many previous reports of low bacterial diversity in hypersaline environments and demonstrates unusually high diversity of presented taxa, some unusual domination of diverse genera not reported before as dominant and identification of previously unknown 16S rRNA sequences. The retrieved 23 bacterial operational taxonomic units (OTUs) affiliated with 15 bacterial genera from four phyla -Firmicutes, 47.5%; Proteobacteria, 23.1%; Bacteroidetes, 22%; Deinococcus-Thermus, 2.4%; and one-candidate division SR1, 4.8%. Representatives of the phylum Firmicutes predominated in the bacterial community with almost half of the retrieved sequences. Almost all clones branched together with cultured halophiles or uncultured clones retrieved from saline niches. Despite of the high salt concentration, some of the closest phylogenetic neighbours were moderate halophiles. New sequences represented 42.3% of bacterial OTUs. Some of them formed separate branches with similarity less than 85%.

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Taxonomic Study of Extreme Halophilic Archaea Isolated from the “Salar de Atacama”, Chile

Cited by 41 publications

References 37 publications

Screening of Hydrolytic Extremozymes in Haloalkaliphilic Archaea by Culture and Molecular-based Methods

Screening of Hydrolytic Extremozymes in Haloalkaliphilic Archaea by Culture and Molecular-based Methods

Isolation, Identification and Extracellular Enzymatic Activity of Culturable Extremely Halophilic Archaea and Bacteria of IncheBoroun Wetland

Phylogenetic analysis of the bacterial community in a crystallizer pond, Pomorie salterns, Bulgaria

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