Phylogenetic analysis of the bacterial community in a crystallizer pond, Pomorie salterns, Bulgaria

Kambourova, Margarita; Tomova, Iva; Boyadzhieva, Ivanka; Radchenkova, Nadja; Vasileva−Tonkova, Evgenia

doi:10.1080/13102818.2016.1265900

Cited by 13 publications

(8 citation statements)

References 38 publications

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“…Thus, our outcome was somehow inconsistent with the majority of results from others, but it must be taken into account that most of these regarded the Santa Pola saltern (Spain), which is considered a study model for hypersaline environments, but is located in a more southerly location than ST. Actually, for different solar salterns over the world, other authors reported quite different assemblages concerning the bacterial fraction of the prokaryotic communities, indicating possible bio-geographic patterns [ 18 , 20 , 21 , 23 , 26 , 27 , 32 , 33 , 34 , 35 , 38 , 39 ]. Among them, some studies regarding the MS of Chile, Mexico, India, Bulgaria, and Spain revealed not only the presence of Proteobacteria in the crystalliser ponds (280%–440‰ of salinity), but also that they were more abundant than Bacteroidetes [ 23 , 27 , 33 , 35 , 38 , 39 ]. In addition, in some cases, Actinobacteria was also found among the major phyla harboured by the crystallization ponds [ 32 , 33 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

“…Various studies have been carried out by culture-independent methods to assess the prokaryotic diversity of MS. However, the majority were based on low-resolution methods (i.e., 16S rDNA clone libraries or Denaturing Gradient Gel Electrophoresis, DGGE) [ 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 ], which returned a limited vision of the diversity, affecting also community structure analysis. In addition, the majority of the investigations are based on one-time sampling [ 17 , 18 , 19 , 21 , 23 , 25 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 ], generating scant information on the prokaryotic taxa fluctuations over time.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, general prokaryotic community pattern and distribution along the salinity gradient within the MS environments have often been described [ 4 , 17 , 22 , 25 , 28 , 31 , 36 , 37 ]. Besides, it has been shown that MS of different geographic areas revealed only partially similar patterns and distribution of prokaryotes [ 18 , 20 , 21 , 23 , 26 , 27 , 32 , 33 , 34 , 35 , 38 , 39 ]. Thus, tracing and identifying common and different features in the prokaryotic assemblages of the worldwide MS is of valuable biological and ecological interest.…”

Section: Introductionmentioning

confidence: 99%

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Spatio-Temporal Variation of the Bacterial Communities along a Salinity Gradient within a Thalassohaline Environment (Saline di Tarquinia Salterns, Italy)

et al. 2021

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The “Saline di Tarquinia” salterns have been scarcely investigated regarding their microbiological aspects. This work studied the structure and composition of their bacterial communities along the salinity gradient (from the nearby sea through different ponds). The communities showed increasing simplification of pond bacterial diversity along the gradient (particularly if compared to those of the sea). Among the 38 assigned phyla, the most represented were Proteobacteria, Actinobacteria and Bacteroidetes. Differently to other marine salterns, where at the highest salinities Bacteroidetes dominated, preponderance of Proteobacteria was observed. At the genus level the most abundant taxa were Pontimonas, Marivita, Spiribacter, Bordetella, GpVII and Lentibacter. The α-diversity analysis showed that the communities were highly uneven, and the Canonical Correspondence Analysis indicated that they were structured by various factors (sampling site, sampling year, salinity, and sampling month). Moreover, the taxa abundance variation in relation to these significant parameters were investigated by Generalized Linear Models. This work represents the first investigation of a marine saltern, carried out by a metabarcoding approach, which permitted a broad vision of the bacterial diversity, covering both a wide temporal span (two years with monthly sampling) and the entire salinity gradient (from the nearby sea up to the crystallisation ponds).

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Spatio-Temporal Variation of the Bacterial Communities along a Salinity Gradient within a Thalassohaline Environment (Saline di Tarquinia Salterns, Italy)

et al. 2021

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“…The microbial diversity at BSF is comparable to that of other dry saline environments. Archaeal communities are often dominated by members of Halobacteria (42–44). Bacterial communities are often dominated by Bacteroidetes ( Salinibacter ) and Proteobacteria (45–47).…”

Section: Discussionmentioning

confidence: 99%

Robust Archaeal and Bacterial Communities Inhabit Shallow Subsurface Sediments of the Bonneville Salt Flats

McGonigle

Bernau

Bowen

et al. 2019

mSphere

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We report the first census of natural microbial communities of the Bonneville Salt Flats (BSF), a perennial salt pan at the Utah-Nevada border. Environmental DNA sequencing of archaeal and bacterial 16S rRNA genes was conducted on samples from multiple evaporite sediment layers collected from the upper 30 cm of the surface salt crust. Our results show that at the time of sampling (September 2016), BSF hosted a robust microbial community dominated by diverse halobacteria and Salinibacter species. Sequences identical to Geitlerinema sp. strain PCC 9228, an anoxygenic cyanobacterium that uses sulfide as the electron donor for photosynthesis, are also abundant in many samples. We identified taxonomic groups enriched in each layer of the salt crust sediment and revealed that the upper gypsum sediment layer found immediately under the uppermost surface halite contains a robust microbial community. In these sediments, we found an increased presence of Thermoplasmatales, Hadesarchaeota, Nanoarchaeaeota, Acetothermia, Desulfovermiculus, Halanaerobiales, Bacteroidetes, and Rhodovibrio. This study provides insight into the diversity, spatial heterogeneity, and geologic context of a surprisingly complex microbial ecosystem within this macroscopically sterile landscape. IMPORTANCE Pleistocene Lake Bonneville, which covered a third of Utah, desiccated approximately 13,000 years ago, leaving behind the Bonneville Salt Flats (BSF) in the Utah West Desert. The potash salts that saturate BSF basin are extracted and sold as an additive for agricultural fertilizers. The salt crust is a well-known recreational and economic commodity, but the biological interactions with the salt crust have not been studied. This study is the first geospatial analysis of microbially diverse populations at this site using cultivation-independent environmental DNA sequencing methods. Identification of the microbes present within this unique, dynamic, and valued sedimentary evaporite environment is an important step toward understanding the potential consequences of perturbations to the microbial ecology on the surrounding landscape and ecosystem.

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“…The microbial diversity at BSF is comparable to that of other dry saline environments. Archaeal communities are often dominated by members of Halobacteriaceae (Di Meglio et al, 2016; Kambourova et al, 2017; Stivaletta et al, 2009). Bacterial communities are often dominated by Bacteroidetes ( Salinibacter ) and Proteobacteria (Rasuk et al, 2014; Stivaletta et al, 2011; Vogt et al, 2017).…”

Section: Disscusionmentioning

confidence: 99%

Robust archaeal and bacterial communities inhabit shallow subsurface sediments of the Bonneville Salt Flats

McGonigle

Bernau

Bowen

et al. 2019

Preprint

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25We report the first census of natural microbial communities of the Bonneville Salt Flats 26 (BSF), a perennial salt pan at the Utah-Nevada border. Environmental DNA sequencing of 27 archaeal and bacterial 16S rRNA genes was conducted on samples from multiple evaporite 28 sediment layers of the surface salt crust. Our results show that at the time of sampling 29 (September 2016), BSF hosted a robust microbial community dominated by diverse 30 Halobacteriaceae and Salinibacter species. Desulfuromonadales from GR-WP33-58 are also 31 abundant in all samples. We identified taxonomic groups enriched in each layer of the salt crust 32 sediment and revealed that the upper gypsum sediment layer found immediately under the 33 uppermost surface halite contains a robust microbial community. We found an increased 34 presence of Thermoplasmatales, Nanohaloarchaeota, Woesearchaeota, Acetothermia, 35 Halanaerobium, Parcubacteria, Planctomycetes, Clostridia, Gemmatimonadetes, Marinilabiaceae 36 and other Bacteroidetes in this upper gypsum layer. This study provides insight into the diversity, 37 spatial heterogeneity, and geologic context of a surprisingly complex microbial ecosystem within 38 this macroscopically-sterile landscape. 39 40 IMPORTANCE 41Over the last ~13,000 years the Pleistocene Lake Bonneville, which covered a large 42 portion of Utah, drained and desiccated leaving behind the Bonneville Salt Flats (BSF). Today 43 BSF is famous for its use as a speedway, which has hosted many land-speed records and a 44 community that greatly values this salty landscape. Additionally, the salts that saturate BSF basin 45 are extracted and sold as an additive for agricultural fertilizers. The salt crust is a well-known 46 recreational and economic commodity, but the roles of microbes in the formation and 47 3 maintenance of the salt crust are generally unknown. This study is the first geospatial analysis of 48 microbial diversity at this site using cultivation-independent environmental DNA sequencing 49 methods. Identification of the microbes present within this unique, dynamic, and valued 50 sedimentary evaporite environment is an important step toward understanding the potential 51 consequences of perturbations to the microbial ecology on the surrounding landscape and 52 ecosystem.53 54 65Human land use has altered many aspects of the hydrology and morphology of the environment 66 that facilitated deposition of the ~2m thick evaporite salt crust that caps BSF, and the amount 67 and extent of salt present at the site have been observed to change through time (Bowen, B.B., 68 Bernau, J., Kipnis, E.L., Lerback, J., Wetterlin, L. and Kleba, 2018; Bowen et al., 2018).69 4Examination of modern and ancient salt pan deposits demonstrates that these 70 environments undergo repeated cycles of desiccation (dry saline pan), flooding (brackish lake), 71 and evaporative concentration (drying to dry saline pan) (Bowen and Benison, 2009; 72 Lowenstein, T.K. and Hardie, 1985). While the desiccation stage is most common, it is 73 repeat...

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Phylogenetic analysis of the bacterial community in a crystallizer pond, Pomorie salterns, Bulgaria

Cited by 13 publications

References 38 publications

Spatio-Temporal Variation of the Bacterial Communities along a Salinity Gradient within a Thalassohaline Environment (Saline di Tarquinia Salterns, Italy)

Spatio-Temporal Variation of the Bacterial Communities along a Salinity Gradient within a Thalassohaline Environment (Saline di Tarquinia Salterns, Italy)

Robust Archaeal and Bacterial Communities Inhabit Shallow Subsurface Sediments of the Bonneville Salt Flats

Robust archaeal and bacterial communities inhabit shallow subsurface sediments of the Bonneville Salt Flats

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