“…Ra1051/98 strain showed high level of ampicillin resistance (MIC > 256 mg l −1 ) and was positive for β-lactamase activity. With the exception of a single report by Shoeib et al (1987), who reported the occurrence of ampicillin resistance in 30 of 90 E. amylovora strains tested, there are no data on ampicillin resistance occurrence in E. amylovora. Our observation indicates that contrary to other regions in the world, E. amylovora strains isolated from the Slovak and Hungarian territory lack detectable antibiotic resistance.…”
a department of general and Quarantine diagnostics, central control and testing institute of Agriculture, haniska, slovakia; b institute of Animal Physiology, slovak Academy of sciences, Košice, slovakia; c faculty of natural sciences, department of chemistry, ABSTRACT Genetic variability of 17 wild strains of γ-proteobacteria isolated from different host plants, locations and seasons, identified via routine diagnostics as Erwinia amylovora, was analysed. The ERIC-PCR confirmed the genetic homogeneity among 15 virulent strains while 2 avirulent strains were genetically distinct. These two avirulent strains differed in their antibiotic susceptibility from all virulent strains. The only avirulent Ra1051/98 strain showed significant ampicillin resistance. All 15 virulent strains were confirmed via the ERIC-PCR and MALDI-TOF MS, as closely related to the reference strain NCPPB 683 of E. amylovora. Avirulent strains were identified as Rahnella aquatilis and L. quercina subsp. britannica (formerly Brenneria quercina), by MALDI-TOF MS and the identification was confirmed by 16S rRNA gene sequence analysis. For the first time, L. quercina subsp britannica was identified in the fire blight-symptomatic samples in Central Europe, and for the first time this bacterium was isolated from the host different than oak.
“…Ra1051/98 strain showed high level of ampicillin resistance (MIC > 256 mg l −1 ) and was positive for β-lactamase activity. With the exception of a single report by Shoeib et al (1987), who reported the occurrence of ampicillin resistance in 30 of 90 E. amylovora strains tested, there are no data on ampicillin resistance occurrence in E. amylovora. Our observation indicates that contrary to other regions in the world, E. amylovora strains isolated from the Slovak and Hungarian territory lack detectable antibiotic resistance.…”
a department of general and Quarantine diagnostics, central control and testing institute of Agriculture, haniska, slovakia; b institute of Animal Physiology, slovak Academy of sciences, Košice, slovakia; c faculty of natural sciences, department of chemistry, ABSTRACT Genetic variability of 17 wild strains of γ-proteobacteria isolated from different host plants, locations and seasons, identified via routine diagnostics as Erwinia amylovora, was analysed. The ERIC-PCR confirmed the genetic homogeneity among 15 virulent strains while 2 avirulent strains were genetically distinct. These two avirulent strains differed in their antibiotic susceptibility from all virulent strains. The only avirulent Ra1051/98 strain showed significant ampicillin resistance. All 15 virulent strains were confirmed via the ERIC-PCR and MALDI-TOF MS, as closely related to the reference strain NCPPB 683 of E. amylovora. Avirulent strains were identified as Rahnella aquatilis and L. quercina subsp. britannica (formerly Brenneria quercina), by MALDI-TOF MS and the identification was confirmed by 16S rRNA gene sequence analysis. For the first time, L. quercina subsp britannica was identified in the fire blight-symptomatic samples in Central Europe, and for the first time this bacterium was isolated from the host different than oak.
“…Judging on the results obtained it can be concluded that all the investigated strains isolated in Yugoslavia, Canada and England from pome fruits (apple, pear and quince) showed homogenous characteristics in all test performed (Table 1). The homogeneity among Erwinia amylovora strains originating from pomaceous and ornamental plants was registered by Vantomme et al (1982), Shoeib et al (1987), Brown et al (1996), Kim et al (1996).…”
Pathogenicity, cultural and biochemical characteristics of the Erwinia amylovora strins of various origin were ivestigated. Six of them originated from apple (Yugoslavia and Canada), and four from pear and quince trees (England and Yugoslavia). No differences were observed in any property among the strains investigated.
“…Ten most virulent and important bacterial plant pathogens based on their pathogenicity level, economic impact, and molecular aspects were listed1. Most devastating bacterial plant pathogens species were isolated in Egypt which belong to Pseudomonas syringae pathovars 3,4,5,6 , Ralstonia solanacearum 7,8,9 , Agrobacterium tumefaciens 9,10,11,12 , Xanthomonas 3,13,14 , Erwinia amylovora 15,16,17,18,19,20 and Xylella fastidiosa 21. Bacterial soft rot soft rots commonly affect vegetables and fruits. It can occur on crops in the eld and on the market.…”
The nanoparticles (NPs) formed by Enterococcus thailandicus, Pseudomonas putida, Marinobacter hydrocarbonoclasticus, and P. geniculate were tested against soft rot/blackleg genera. The effects of NPs recorded on bacterial DNA, proteins, and carbohydrates concentration of Pectobacterium carotovorum subsp. carotovorum, Enterobacter cloacae (soft rot), and Dickeya solani (soft rot/blackleg). Treated cells showed degradation in isolated DNA and decreased proteins and carbohydrates concentration compared with untreated cells. Using Scanning Electron Microscope (SEM), the treated cells showed collapsed and small pits in the cell wall. Using Transmission Electron Microscope (TEM), internal changes showed penetration of NPs inside the tested bacterial cells, the appearance of periplasmic space, formation of vacuoles, and condensation of cytoplasm.
Disease severity ex vivo of potato tuber infected with tested genera demonstrated that NPs treatment didn't show any rotted tissue compared with untreated. The ability to uptake and accumulate FeNPs from the soil in potato (Solanum tuberosum) seedlings; Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) was used. ICP-OES recorded an increase in iron content of treated potato (Solanum tuberosum) seedlings with NPs, compared with untreated. FeNPs can be used to control soft rot/blackleg diseases; instead of copper pesticides. It could be a new, and alternative approach to traditional disease management methods and increase the plant's nutritional value.
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