Taxonomic studies on Debaryomyces hansenii (Zopf) Lodder et Kreger-van Rij and related species. I. Chemotaxonomic investigations.

Abstract

Taxonomic studies on Debaryomyces hansenii (ZOPF) Lodder et Kregervan Rij and related yeast species were carried out using chemotaxonomic methods including DNA base composition, DNA-DNA hybridization, ubiquinone system, proton magnetic resonance spectrum of mannan, and serological methods. The 50 strains employed were classified into 3 groups, I, II, and III, based on DNA-DNA hybridization experiments. These groups were considered to represent three distinct species. Group I consisted of two subgroups, Ia and Ib, which were considered to represent two distinct varieties. These groups were also characterized by proton magnetic resonance spectra of alkali-extracted mannans and cell surface antigens, however, they could not be discriminated by DNA base composition and ubiquinone systems, in addition to the taxonomic criteria commonly employed in yeast taxonomy. Group Ia comprised 34 strains including the type or authentic strains of D.

Molecular characterization of the closely related Debaryomyces species: Proposition of D. vindobonensis sp. nov. from a municipal wastewater treatment plant

“…2). In a taxonomic study on D. hansenii and related species, Nakase and Suzuki (1985a) combined D. subglobosus CBS 792 T and D. fabryi in the same group on the basis of the high DNA:DNA hybridization value and separated them from the D. hansenii and D. nepalensis groups. These authors also observed that D. nepalensis was difficult to discriminate from D. hansenii var.…”

Candida stellimalicola, a new species of anamorphic yeast isolated from star apple in Thailand.

“…The isolation and purification of DNAs were carried out according to the procedures previously reported (6). DNA base composition was determined by the thermal denaturation temperature (Tm) as previously reported using the equation of Marmur and Doty (5) and corrected to the G + C value of the reference DNA.…”

“…DNA from the type strain of Candida parapsilosis (JCM 1785 = CBS 604), whose mol% G + C is 40.8, was used as the reference. DNA-DNA hybridization experiment was carried out using membrane filter technique according to the method of Kaneko (3) as previously described by Nakase and Suzuki (6). Ubiquinones were extracted with hexane after saponification of intact cells by the method of Yamada and Kondo (14), then purified by preparative thin-layer chromatography using Merck Kieselgel 60 F254 plates (Merck, Darmstadt, Germany) and benzene as a solvent system.…”

Candida fragi, a new species of anamorphic yeast isolated from fermenting strawberry.

“…DNAs were extracted and purified according to the method described in the previous papers (7,9). DNA-DNA hybridization was carried out using the membrane filter technique of Kaneko (2), as described by Nakase and Suzuki (7). RESULTS …”