Taxonomic implications of Prototheca and Chlorella cell wall polysaccharide characterization

Conte, Mario; Pore, R. S.

doi:10.1007/bf00411203

Cited by 35 publications

(8 citation statements)

References 11 publications

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“…NORTHCOTE et al (1958) separated from the cell walls of Chlorella pyrenoidosa a component made up mainly of glucose and galactose. CONTE and PORE (1973) concluded that these sugars were present as heteroglycans in the cell walls of that and other species of Chlorella and Proto theca. Glucosamine was detected in their material by NORTHCOTE et al (1958) and also as a major constituent by TAKEDA and HIROKAWA (1978) in C. ellipsoidea.…”

Section: Chlorophyceaementioning

confidence: 97%

Algal Walls — Composition and Biosynthesis

Percival¹,

McDowell²

1981

Plant Carbohydrates II

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Section: Chlorophyceaementioning

confidence: 97%

Algal Walls — Composition and Biosynthesis

Percival¹,

McDowell²

1981

Plant Carbohydrates II

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“…Prototheca is a yeast‐like genus that has been accepted as the apochlorotic equivalent of Chlorella since its original description by Wilhelm Krüger [1]. Subsequent investigators have inferred its closer relationship to the photosynthetic alga Auxenochlorella protothecoides than to other unicellular green algae by means of chemotaxonomic strategies [2–5]. Since the publication of the first phylogram inferred from the nuclear‐encoded small‐subunit (SSU) ribosomal RNA gene (rDNA) sequences that included Chlorella and Prototheca species [6], comparative nucleotide sequence analyses of this phylogenetically informative site have proved the predicted monophyletic relationships of A. protothecoides , Prototheca moriformis , P. wickerhamii , and P. zopfii [6–9].…”

Section: Introductionmentioning

confidence: 99%

Phylogeny of the non-photosynthetic green micro-algal genus Prototheca (Trebouxiophyceae, Chlorophyta) and related taxa inferred from SSU and LSU ribosomal DNA partial sequence data

Ueno

Urano

Suzuki

2003

FEMS Microbiology Letters

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All five species in the heterotrophic micro-algal genus Prototheca and their relatives were compared for the extent of nucleotide divergence in the nuclear small-subunit (SSU) and in the 5' end of large-subunit (LSU) ribosomal RNA genes (rDNAs). Phylogenetic analysis based on combined SSU and LSU rDNA sequence alignment was implemented with the neighbor-joining, the maximum-parsimony, and the maximum-likelihood methods. The relationships among the species of Prototheca based on this data set were largely concordant with those inferred from SSU or LSU rDNA sequences alone. The obtained phylogenetic trees indicated that P. stagnora and P. ulmea should be regarded as different species and that both of the species as well as P. moriformis were placed in a cluster represented by P. zopfii, whereas P. wickerhamii was not directly grouped together with the other members of Prototheca and was more closely related to the autotrophic alga Auxenochlorella protothecoides. Therefore, the genus Prototheca is paraphyletic in its present circumscription; and these conclusions lead us to propose the transfer of P. wickerhamii to Auxenochlorella or to a new genus. On the basis of nucleotide sequence similarities, unlike SSU rDNA, the LSU rDNA region examined in this study appeared to be variable in recognizing a heterogeneity within a single species P. zopfii, which had been shown earlier in a chemotaxonomic study.

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“…Others have used serological cross-reactions (Sanders et al 1971, Maruyama 1977, Kümmel and Kessler 1980 for the identification and classification of Chlorella strains. A third categorization has been based on the ultrastructure and chemical composition of the cell wall (Atkinson et al 1972, Conte and Pore 1973, Yamada and Sakaguchi 1982, Blumreisinger et al 1983, Takeda 1991, 1996a and pyrenoid ultrastructure (Ikeda and Takeda 1995). The most acknowledged and practicable system for species delimitation in Chlorella proved to be a chemotaxonomical classification scheme combining biochemical and physiological characters (Kessler and Soeder 1962, Kessler 1982, 1992.…”

mentioning

confidence: 99%

BIOCHEMICAL TAXONOMY AND MOLECULAR PHYLOGENY OF THE GENUS CHLORELLA SENSU LATO (CHLOROPHYTA)

Huss

Frank

Hartmann

et al. 1999

Journal of Phycology

276

250

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A multimethod approach was used to characterize unicellular green algae that were traditionally assigned to the genus Chlorella Beijerinck and to resolve their phylogenetic relationships within the Chlorophyta. Biochemical, physiological, and ultrastructural characters, together with molecular data such as DNA base composition and DNA hybridization values, were compared with a molecular phylogeny based on complete 18S rRNA sequences. Our results show that Chlorella taxa are dispersed over two classes of chlorophytes, the Trebouxiophyceae and the Chlorophyceae. We propose that only four species should be kept in the genus Chlorella (Chlorophyta, Trebouxiophyceae)

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Taxonomic implications of Prototheca and Chlorella cell wall polysaccharide characterization

Cited by 35 publications

References 11 publications

Algal Walls — Composition and Biosynthesis

Algal Walls — Composition and Biosynthesis

Phylogeny of the non-photosynthetic green micro-algal genus Prototheca (Trebouxiophyceae, Chlorophyta) and related taxa inferred from SSU and LSU ribosomal DNA partial sequence data

BIOCHEMICAL TAXONOMY AND MOLECULAR PHYLOGENY OF THE GENUS CHLORELLA SENSU LATO (CHLOROPHYTA)

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