Taxonomic diversity and toxicological assessment of Cyanobacteria in Moroccan inland waters

Douma, Mountasser; Loudiki, M.; Oudra, Brahim; Mouhri, Khadija; Ouahid, Youness; Campo, Francisca F. del

doi:10.7202/037781ar

Cited by 11 publications

(8 citation statements)

References 38 publications

(34 reference statements)

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“…cf. biceps is found mainly attached to substrate (Komárek and Anagnostidis, 2005;Douma et al, 2009;Klemenčič et al, 2010).…”

Section: Resultsmentioning

confidence: 99%

“…cf. biceps is a species that lives primarily attached to substrate (Komárek and Anagnostidis, 2005;Douma et al, 2009;Klemenčič et al, 2010) and therefore belonging to MP codon. In the Castanhão reservoir, the most remarkable environmental change associated to the emergence of Pseudanabaena sp.1 in phase II (absent in phase I) was the increase in Secchi depth and the decrease in ammonium-N.…”

Section: Discussionmentioning

confidence: 99%

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Assessing temporal and spatial variability of phytoplankton composition in a large reservoir in the Brazilian northeastern region under intense drought conditions

et al. 2017

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The present study was carried out in Castanhão Reservoir, a large aquatic system in the Brazilian semi-arid region that serves multiples uses as water drinking supply and intensive fish-cage aquaculture site. In order to understand the effects of environmental conditions on the spatial and temporal variability of the phytoplankton functional groups (FG) and the main 'characterizing taxa', sub-superficial water samples were collected from March 2012 to August 2013, a period distinguished by the continuous drop in reservoir volume due to rainfall shortage. Eighteen functional groups and 102 total phytoplankton taxa were found in the Castanhão reservoir during the study. No significant differences were observed relative to spatial variation of total phytoplankton composition throughout the reservoir (PERMANOVA, P>0.05). On the other hand, according to cluster analysis results, three temporal phases have been identified (Similarity Profile, P<0.05), based on 102 phytoplankton taxa. The 'characterizing taxa' was found using the Similarity Percentage procedure (cut-off 90%), being thus defined as those taxa that contributed the most to the similarity within each temporal phase. Nineteen 'characterizing taxa' described the Castanhão reservoir, with predominance of those typical of mixing and turbidity conditions. Cyanobacteria dominated through the three temporal phases. According to the redundancy analysis, nutrient availability and water transparency were found to influence the phytoplankton temporal dynamics. The phase I (rainy season) was most represented by Planktolyngbya minor/Pl. limnetica (FG=S1), which reached best performance under strongly decreased phosphate-P concentrations and low water transparency. In phase II (dry season), Pseudanabaena sp.1 (FG=?) outcompeted other cyanobacteria probably due the increase in water transparency and decrease in ammonium-N. Finally, in phase III (rainy season) the decrease of water transparency triggered a recovery of shade-adapted cyanobacteria, but at this time mostly represented by Pseudanabaena limnetica (FG=S1). Phase III was also distinctive from the other ones by the highest Nitrate-N and phosphate-P concentrations related to thermocline disruption, which favored an increase in total phytoplankton biomass recorded by the augment of green algae density (FGs=X1, J and F). We concluded that the temporal dynamics of phytoplankton composition was associated to environmental changes in Castanhão Reservoir from 2012 to 2013, which were driven by seasonal climate variation from region (rainy/dry seasons), as well as, by the reduction in reservoir volume that resulted in the disruption of the thermocline, water mixing and an increase in inorganic P and N.

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“…cf. biceps is found mainly attached to substrate (Komárek and Anagnostidis, 2005;Douma et al, 2009;Klemenčič et al, 2010).…”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Assessing temporal and spatial variability of phytoplankton composition in a large reservoir in the Brazilian northeastern region under intense drought conditions

et al. 2017

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“…None of them could also be classified as a new variant of the known peptides group commonly occurring in cyanobacterial taxa. So far, the only peptides identified in Pseudanabaena species were microcystins [ 13 , 14 , 15 , 16 , 17 , 18 ]. Based on the analyses of MS/MS spectra it can be concluded that peptides detected in P. galeata CCNP1313 are composed of proteinogenic amino acids.…”

Section: Discussionmentioning

confidence: 99%

“…As cellular extracts were used in these studies, the active agents could not be identified. Some Pseudanabaena chemotypes produce known toxins, such as microcystins [ 13 , 14 , 15 , 16 , 17 , 18 ], anatoxin-a [ 18 ], and saxitoxin [ 18 ]; therefore, these compounds could be responsible for the observed effects. Pseudanabaena has also been explored as a source of metabolites with biotechnological and pharmaceutical potential [ 19 , 20 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Pseudanabaena galeata CCNP1313—Biological Activity and Peptides Production

Cegłowska

Szubert

Grygier

et al. 2022

Toxins

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Even cyanobacteria from ecosystems of low biodiversity, such as the Baltic Sea, can constitute a rich source of bioactive metabolites. Potent toxins, enzyme inhibitors, and anticancer and antifungal agents were detected in both bloom-forming species and less commonly occurring cyanobacteria. In previous work on the Baltic Pseudanabaena galeata CCNP1313, the induction of apoptosis in the breast cancer cell line MCF-7 was documented. Here, the activity of the strain was further explored using human dermal fibroblasts, African green monkey kidney, cancer cell lines (T47D, HCT-8, and A549ACE2/TMPRSS2) and viruses (SARS-CoV-2, HCoV-OC43, and WNV). In the tests, extracts, chromatographic fractions, and the main components of the P. galeata CCNP1313 fractions were used. The LC-MS/MS analyses of the tested samples led to the detection of forty-five peptides. For fourteen of the new peptides, putative structures were proposed based on MS/MS spectra. Although the complex samples (i.e., extracts and chromatographic fractions) showed potent cytotoxic and antiviral activities, the effects of the isolated compounds were minor. The study confirmed the significance of P. galeata CCNP1313 as a source of metabolites with potent activity. It also illustrated the difficulties in assigning the observed biological effects to specific metabolites, especially when they are produced in minute amounts.

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“…Benthic cyanobacteria that produce microcystins (MCs) were first detected and characterised in communities in alpine lakes [ 1 ]. Since then, their presence in benthic communities in Spain [ 2 , 3 ], California [ 4 ], Egypt [ 5 ], Morocco [ 6 , 7 ], New Zealand [ 8 ], and other countries [ 9 ] has been reported. More recently, the presence of benthic cyanobacteria has been demonstrated in 30% of fordable rivers in California, and the number of potentially toxic genera has increased [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Are We Underestimating Benthic Cyanotoxins? Extensive Sampling Results from Spain

Uriza

Asencio

Aboal

2017

Toxins

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Microcystins (MCs) are potent hepatotoxins, and their presence in water bodies poses a threat to wildlife and human populations. Most of the available information refers to plankton, and much less is known about microcystins in other habitats. To broaden our understanding of the presence and environmental distribution of this group of toxins, we conducted extensive sampling throughout Spain, under a range of conditions and in distinct aquatic and terrestrial habitats. More than half of the tested strains were toxic; concentrations of the hepatotoxin were low compared with planktic communities, and the number of toxic variants identified in each sample of the Spanish strains ranged from 1–3. The presence of microcystins LF and LY (MC-LF and MC-LY) in the tested samples was significant, and ranged from 21.4% to 100% of the total microcystins per strain. These strains were only detected in cyanobacteria Oscillatoriales and Nostocales. We can report, for the first time, seven new species of microcystin producers in high mountain rivers and chasmoendolithic communities. This is the first report of these species in Geitlerinema and the confirmation of Anatoxin-a in Phormidium uncinatum. Our findings show that microcystins are widespread in all habitat types, including both aerophytic and endolithic peat bogs and that it is necessary to identify all the variants of microcystins in aquatic bodies as the commonest toxins sometimes represent a very low proportion of the total.

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Taxonomic diversity and toxicological assessment of Cyanobacteria in Moroccan inland waters

Cited by 11 publications

References 38 publications

Assessing temporal and spatial variability of phytoplankton composition in a large reservoir in the Brazilian northeastern region under intense drought conditions

Assessing temporal and spatial variability of phytoplankton composition in a large reservoir in the Brazilian northeastern region under intense drought conditions

Pseudanabaena galeata CCNP1313—Biological Activity and Peptides Production

Are We Underestimating Benthic Cyanotoxins? Extensive Sampling Results from Spain

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