Tasked with a Challenging Objective: Why Do Neutrophils Fail to Battle Pseudomonas aeruginosa Biofilms

Geddes‐McAlister, Jennifer; Kugadas, Abirami; Gadjeva, Mihaela

doi:10.3390/pathogens8040283

Cited by 18 publications

(10 citation statements)

References 77 publications

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“…Wang et al [98] reported that even Psl can interact with eDNA and, in in vitro grown P. aeruginosa biofilms, form fiber-like structures, where the two polymers colocalize. Psl-based fibers were further confirmed in vivo and found to emanate from the body of the biofilms [99]. However, the nature of the interaction between the Psl and eDNA remains unexplained.…”

Section: Interaction Of Edna With Biofilm Exopolysaccharidesmentioning

confidence: 86%

Extracellular DNA (eDNA). A Major Ubiquitous Element of the Bacterial Biofilm Architecture

Campoccia

Montanaro

Arciola

2021

IJMS

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After the first ancient studies on microbial slime (the name by which the biofilm matrix was initially indicated), multitudes of studies on the morphology, composition and physiology of biofilms have arisen. The emergence of the role that biofilms play in the pathogenesis of recalcitrant and persistent clinical infections, such as periprosthetic orthopedic infections, has reinforced scientific interest. Extracellular DNA (eDNA) is a recently uncovered component that is proving to be almost omnipresent in the extracellular polymeric substance (EPS) of biofilm. This macromolecule is eliciting unprecedented consideration for the critical impact on the pathogenesis of chronic clinical infections. After a systematic review of the literature, an updated description of eDNA in biofilms is presented, with a special focus on the latest findings regarding its fundamental structural role and the contribution it makes to the complex architecture of bacterial biofilms through interactions with a variety of other molecular components of the biofilm matrix.

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Section: Interaction Of Edna With Biofilm Exopolysaccharidesmentioning

confidence: 86%

Extracellular DNA (eDNA). A Major Ubiquitous Element of the Bacterial Biofilm Architecture

Campoccia

Montanaro

Arciola

2021

IJMS

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“…The recognition of P. aeruginosa pathogen-associated molecular patterns (PAMPs) elicits a potent inflammatory response, which is critical for the recruitment of neutrophils and macrophages, facilitating the bacterial clearance ( Figure 2). However, an optimal host response is essential; a weak response with inefficient infiltration of phagocytic cells leads to unsuccessful bacterial killing and clearance, while an excessive response causes host tissue damage [49,[70][71][72][73][74]. Massive recruitment of neutrophils into the infected respiratory tract is a hallmark of P. aeruginosa infection.…”

Section: The Innate Immune Responsementioning

confidence: 99%

Understanding Pseudomonas aeruginosa–Host Interactions: The Ongoing Quest for an Efficacious Vaccine

Sainz-Mejías

Jurado‐Martín

McClean

2020

Cells

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Pseudomonas aeruginosa is a leading cause of chronic respiratory infections in people with cystic fibrosis (CF), bronchiectasis or chronic obstructive pulmonary disease (COPD), and acute infections in immunocompromised individuals. The adaptability of this opportunistic pathogen has hampered the development of antimicrobial therapies, and consequently, it remains a major threat to public health. Due to its antimicrobial resistance, vaccines represent an alternative strategy to tackle the pathogen, yet despite over 50 years of research on anti-Pseudomonas vaccines, no vaccine has been licensed. Nevertheless, there have been many advances in this field, including a better understanding of the host immune response and the biology of P. aeruginosa. Multiple antigens and adjuvants have been investigated with varying results. Although the most effective protective response remains to be established, it is clear that a polarised Th2 response is sub-optimal, and a mixed Th1/Th2 or Th1/Th17 response appears beneficial. This comprehensive review collates the current understanding of the complexities of P. aeruginosa-host interactions and its implication in vaccine design, with a view to understanding the current state of Pseudomonal vaccine development and the direction of future efforts. It highlights the importance of the incorporation of appropriate adjuvants to the protective antigen to yield optimal protection.

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“…And the synthesis of extracellular polymeric substances (EPS) forms sticky and tangled fibers that connect bacteria cells to each other and to various surface[58]. Thus, we speculate that the biofilm lifestyle acts as a defense barrier that protects the bacteria embedded in the EPS matrix against innate host defense[59] and shear caused by fluid flow[60]. In addition, EPS produced on the surface of cancer cells inhibits the adhesion of cancer cells to endothelial cells, resulting in metastasis disruption[42, 61].…”

Section: Discussionmentioning

confidence: 99%

Programming the lifestyles of engineered bacteria for cancer therapy

Zhang

Gao

et al. 2022

Preprint

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Bacteria can be genetically engineered to act as therapeutic delivery vehicles in the treatment of tumors, killing cancer cells or activating the immune system. This is known as Bacteria-Mediated Cancer Therapy (BMCT). Tumor invasion, colonization and tumor regression are major biological events, which are directly associated with antitumor effects and are uncontrollable due to the influence of tumor microenvironments during the BMCT process. Here, we developed a genetic circuit for dynamically programming bacterial lifestyles (planktonic, biofilm or lysis), to precisely manipulate the process of bacterial adhesion, colonization and drug release in BMCT process, via hierarchical modulation of the lighting power density (LPD) of near-infrared (NIR) light. The deep tissue penetration of NIR offers us a modality for spatiotemporal and noninvasive control of bacterial genetic circuits in vivo. By combining computational modeling with high throughput characterization device, we optimized the genetic circuits in engineered bacteria to program the process of bacterial lifestyle transitions by altering the illumination scheme of NIR. Our results showed that programming intratumoral bacterial lifestyle transitions allows precise control of multiple key steps throughout the BMCT process, and therapeutic efficacy can be greatly improved by controlling the localization and dosage of therapeutic agents via optimizing the illumination scheme.

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Tasked with a Challenging Objective: Why Do Neutrophils Fail to Battle Pseudomonas aeruginosa Biofilms

Cited by 18 publications

References 77 publications

Extracellular DNA (eDNA). A Major Ubiquitous Element of the Bacterial Biofilm Architecture

Extracellular DNA (eDNA). A Major Ubiquitous Element of the Bacterial Biofilm Architecture

Understanding Pseudomonas aeruginosa–Host Interactions: The Ongoing Quest for an Efficacious Vaccine

Programming the lifestyles of engineered bacteria for cancer therapy

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