Targeting quorum sensing mechanism: An alternative anti-virulent strategy for the treatment of bacterial infections

Santhakumari, Sivasubramanian; Ravi, Arumugam Veera

doi:10.1016/j.sajb.2018.09.028

Cited by 31 publications

(12 citation statements)

References 46 publications

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“…Another therapeutic, yet non-antibiotic, strategy is targeting bacterial virulence controlled by quorum sensing processes. The quorum-sensing mechanism mediated by signal molecules regulates the expression of virulence genes in the majority of pathogenic bacteria, meaning that quorum-sensing inhibitors are expected to be one of the best alternatives to antibiotics 9 , 10 . Autoinducers, self-secreted signal molecules, are regulated by a density-dependent synchronized gene expression system during quorum sensing 11 .…”

Section: Introductionmentioning

confidence: 99%

“…Autoinducers, self-secreted signal molecules, are regulated by a density-dependent synchronized gene expression system during quorum sensing 11 . Biofilm formation, toxin production and other virulence factors are controlled by quorum sensing and the production of virulence factors can weaken the balance of host defense mechanisms 9 . Initiation of toxin production occurs when extracellular signaling and communication indicates that a threshold population of bacteria has been achieved 12 .…”

Section: Introductionmentioning

confidence: 99%

“…Initiation of toxin production occurs when extracellular signaling and communication indicates that a threshold population of bacteria has been achieved 12 . Inhibition of quorum sensing induced by secondary plant metabolites can significantly attenuate bacterial virulence and substantially enhance vulnerability to conventional antibiotics and to the immune system 9 , 12 – 14 .…”

Section: Introductionmentioning

confidence: 99%

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Targeting ESKAPE pathogens with anti-infective medicinal plants from the Greater Mpigi region in Uganda

Schultz

Anywar

Tang

et al. 2020

Sci Rep

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Antibiotic resistance poses one of the greatest threats to global health today; conventional drug therapies are becoming increasingly inefficacious and limited. We identified 16 medicinal plant species used by traditional healers for the treatment of infectious and inflammatory diseases in the Greater Mpigi region of Uganda. Extracts were evaluated for their ability to inhibit growth of clinical isolates of multidrug-resistant ESKAPE pathogens. Extracts were also screened for quorum quenching activity against S. aureus, including direct protein output assessment (δ-toxin), and cytotoxicity against human keratinocytes (HaCaT). Putative matches of compounds were elucidated via LC-FTMS for the best-performing extracts. These were extracts of Zanthoxylum chalybeum (Staphylococcus aureus: MIC: 16 μg/mL; Enterococcus faecium: MIC: 32 μg/mL) and Harungana madagascariensis (S. aureus: MIC: 32 μg/mL; E. faecium: MIC: 32 μg/mL) stem bark. Extracts of Solanum aculeastrum root bark and Sesamum calycinum subsp. angustifolium leaves exhibited strong quorum sensing inhibition activity against all S. aureus accessory gene regulator (agr) alleles in absence of growth inhibition (IC 50 values: 1-64 μg/mL). The study provided scientific evidence for the potential therapeutic efficacy of these medicinal plants in the Greater Mpigi region used for infections and wounds, with 13 out of 16 species tested being validated with in vitro studies. The rise of antimicrobial resistance (AMR) requires mobilization of political, financial and research investment due to its emergence as a global health hazard that threatens the ability to treat infectious diseases 1. According to the World Health Organization, AMR poses "one of the biggest threats to global health, food security, and development today" and can affect anyone in any country and of any age 2. Today, AMR already accounts for 700,000 deaths annually. By 2050, this figure is estimated to reach more than 10 million deaths per year, which is more people than currently die from cancer 3. Because effective antibiotics are critical for treatment of bacterial infections and for procedures where there is a high risk of infection, e.g. surgery, new anti-infectives are needed to overcome this global threat 4. The issue of resistance is not uniformly spread across all bacteria 5. Six species have been identified by the Infectious Disease Society of America (IDSA) as being especially dangerous due to their potential multidrug resistance mechanisms and virulence. They are referred to as 'ESKAPE' pathogens, which is an acronym for Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter species. This group of pathogenic bacteria encompasses both Gram-negative and Gram-positive species that are capable of 'escaping' bactericidal action of conventional

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Targeting ESKAPE pathogens with anti-infective medicinal plants from the Greater Mpigi region in Uganda

Schultz

Anywar

Tang

et al. 2020

Sci Rep

View full text Add to dashboard Cite

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“…The authors also emphasized the ability of the extract to inhibit 79% ± 3.5% quorum sensing formation in concentrations of 0.750 μg/mL, which is commonly performed by strains of these bacteria. The use of compounds with anti-biofilm activity has been reported as a possible alternative therapy for treating various infections as a solution to the phenomenon of microbial resistance ( Santhakumari and Ravi, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Potential of Streptomyces ansochromogenes (PB3) Isolated From a Plant Native to the Amazon Against Pseudomonas aeruginosa

et al. 2020

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The objective of this study was to evaluate the antibacterial action of filamentous bacteria isolated from the Byrsonima crassifolia leaf. An endophytic bacterium has been identified by classical and molecular techniques as Streptomyces ansochromogene . Screening for antibacterial action against pathogens with medical relevance ( Klebsiella pneumoniae ATCC 700603, Pseudomonas aeruginosa ATCC 15692, Staphylococcus aureus ATCC 6538, Corynebacterium diphtheriae ATCC 27012, Mycobacterium abscessus , Cryptococcus gattii ATCC 24065, and Cryptococcus neoformans ATCC 24067) demonstrated activity against the bacterium P. aeruginosa ATCC 0030 with inhibition diameter zones (IDZ) of 17.6 ± 0.25 mm in the preliminary screening in solid medium. After fermentation in liquid medium, an IDZ of 19.6 ± 0.46 mm and a minimum inhibitory concentration (MIC) of 0.5 mg/mL were detected. The antibiofilm action was observed with 100% inhibition of biofilm formation at a concentration of 0.250 mg/mL. When the infection curve was prepared, it was observed that the metabolite was effective in protecting the larvae of Tenebrio molitor . The metabolite does not show toxicity for eukaryotic cells. The leishmanicidal activity demonstrated that the metabolite presented a dose-dependent effect on the promastigotes forms of Leishmania amazonensis growth and the estimated IC 50 /72 h was 71.65 ± 7.4 μg/mL. Therefore, it can be concluded that the metabolite produced by the endophytic bacterium Streptomyces sp. is promising for future use as an alternative strategy against bacterial resistance.

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“…Bacterial pathogens contain many structurally and functionally distinct toxins and are, therefore, very significant in disease progress [148]. Surprisingly enough, some studies indicate that RSV interferes with toxins' expression [149]. In V. vulnificus, RtxA1 is an essential multifunctional cytotoxic toxin for lethality in mice, and the treatment with RSV decreases the expression of rtxA1 [136].…”

Section: Antivirulence Propertiesmentioning

confidence: 99%

A Comprehensive Study on the Antimicrobial Properties of Resveratrol as an Alternative Therapy

Abedini

Khodadadi

Zeinalzadeh

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Resveratrol is a polyphenolic antioxidant whose possible health benefits include anticarcinogenic, antiaging, and antimicrobial properties that have gained significant attention. The compound is well accepted by individuals and has been commonly used as a nutraceutical in recent decades. Its widespread usage makes it essential to study as a single agent as well as in combination with traditional prescription antibiotics as regards to antimicrobial properties. Resveratrol demonstrates the action of antimicrobials against a remarkable bacterial diversity, viruses, and fungus. This report explains resveratrol as an all-natural antimicrobial representative. It may modify the bacterial virulence qualities resulting in decreased toxic substance production, biofilm inhibition, motility reduction, and quorum sensing disturbance. Moreover, in conjunction with standard antibiotics, resveratrol improves aminoglycoside efficacy versus Staphylococcus aureus, while it antagonizes the deadly function of fluoroquinolones against S. aureus and also Escherichia coli. The present study aimed to thoroughly review and study the antimicrobial potency of resveratrol, expected to help researchers pave the way for solving antimicrobial resistance.

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Targeting quorum sensing mechanism: An alternative anti-virulent strategy for the treatment of bacterial infections

Cited by 31 publications

References 46 publications

Targeting ESKAPE pathogens with anti-infective medicinal plants from the Greater Mpigi region in Uganda

Targeting ESKAPE pathogens with anti-infective medicinal plants from the Greater Mpigi region in Uganda

Antimicrobial Potential of Streptomyces ansochromogenes (PB3) Isolated From a Plant Native to the Amazon Against Pseudomonas aeruginosa

A Comprehensive Study on the Antimicrobial Properties of Resveratrol as an Alternative Therapy

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