Targeting and imaging of monocyte-derived macrophages in rat's injured artery following local delivery of liposomal quantum dots

Aizik, Gil; Waiskopf, Nir; Agbaria, Majd; Ben-David-Naim, Meital; Nordling-David, Mirjam M.; Jbara-Agbaria, Doaa; Banin, Uri; Golomb, Gershon

doi:10.1016/j.jconrel.2019.12.009

Cited by 14 publications

(7 citation statements)

References 65 publications

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“…However, in some cases, the macrophages are unrestrained and induce exaggerated phagocytosis that causes various inflammatory disorders , such as atherosclerosis, obesity, autoimmune diseases, etc., or contrary, sustain hampered phagocytic activities (i.e., Gaucher’s disease) and expose the individuals to increased risk of infections . These structural/physiological changes can be tracked and monitored by the QDs-based fluorescent bionanoprobes, which exhibit superior optoelectronic and photophysical features when compared to the widely studied conventional organic fluorophores and fluorescent proteins.…”

Section: Introductionmentioning

confidence: 99%

Orange-Emitting ZnSe:Mn²⁺ Quantum Dots as Nanoprobes for Macrophages

Khan

Uchiyama

Khan³

et al. 2020

ACS Appl. Nano Mater.

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The biocompatibility, bionanointeraction, uptake efficiency, and entry pathway of luminescent nanomaterials are the key factors to understand development of an efficient bionanoprobe. The foremost objective of this work is to explore the potential of 3-mercaptopropionic acid (3-MPA) capped ZnSe:xMn 2+ (x = 5, 10, and 15 mol %) quantum dots (QDs) for the development of bionanoprobe used in future biological and clinical applications. For this purpose, highly intense orangeemitting activator Mn 2+ ion doped ZnSe QDs were synthesized via a high-temperature organometallic method and rendered watersoluble by a ligand exchange approach. The morphological and physicochemical characterizations displayed the ultrasmall zincblend cubic crystal structure of QDs with an elliptical shape nanocrystals and average diameter of 4 nm. The luminescent nanomaterials exhibited orange emission centered at 584 nm under excitation at 385 nm. The biocompatibility, time-dependent cellular uptake, and the uptake mechanism of QDs were studied in RAW 264.7 macrophages, accomplished by various cytotoxicity assays, CytoViva hyperspectral enhanced dark-field and dual-mode fluorescence (DMF) microscopy, and transmission electron microscopy (TEM) images. The cytotoxicity study did not confirm any noticeable deleterious effect of QDs within incubation for 6 h. The fluorescence images of cells incubated with QDs showed efficient emission, which is a manifestation that QDs are photochemically stable in the intracellular environment. The cellular uptake findings demonstrated that the QDs were predominantly internalized via clathrin-and caveolae-mediated pathways. After the uptake, QDs aggregates appeared inside the vesicles in the cytoplasm, and their number and size gradually increased as a function of time. Nevertheless, the fluorescent QDs presented remarkable colloidal stability in various media, biocompatibility within the designated time, efficient time-dependent uptake, and distinct entry pathway in RAW macrophages, suggesting promising candidates to explore for the development of future bionanoprobes.

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Section: Introductionmentioning

confidence: 99%

Orange-Emitting ZnSe:Mn²⁺ Quantum Dots as Nanoprobes for Macrophages

Khan

Uchiyama

Khan³

et al. 2020

ACS Appl. Nano Mater.

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“…This disease-site-specific release increases the drug efficacy and reduces side effects due to systemic administration. It has been reported that the ability to target ligands to nanomaterials enables cell specificity of therapeutic genes/drugs ( Arias et al, 2018 ; Yang et al, 2018 ; Aizik et al, 2020 ; Lockhart et al, 2021 ). Additionally, the vascular endothelium is an effective target for vascular-targeted therapy.…”

Section: Intravascular Targeting Nanoparticlesmentioning

confidence: 99%

Advances in nanomaterial-based targeted drug delivery systems

Cheng

Xie

Sun

2023

Front. Bioeng. Biotechnol.

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Nanomaterial-based drug delivery systems (NBDDS) are widely used to improve the safety and therapeutic efficacy of encapsulated drugs due to their unique physicochemical and biological properties. By combining therapeutic drugs with nanoparticles using rational targeting pathways, nano-targeted delivery systems were created to overcome the main drawbacks of conventional drug treatment, including insufficient stability and solubility, lack of transmembrane transport, short circulation time, and undesirable toxic effects. Herein, we reviewed the recent developments in different targeting design strategies and therapeutic approaches employing various nanomaterial-based systems. We also discussed the challenges and perspectives of smart systems in precisely targeting different intravascular and extravascular diseases.

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“…In a recent study, targeting and imaging of monocytederived macrophages followed by local delivery of liposomal QDs in rat's injured artery with no systemic biodistribution of LipQDs have been performed. [188] Scientists believe that with the improvement of a new form of QDs with low toxicity and complex functionalities, these materials will find more multipurpose applications. In this sense, highly luminescent cadmiumfree CuInSe 2 /ZnS core/shell QDs conjugated with amino acids www.advancedsciencenews.com www.biotechnology-journal.com of cysteine-glycine-lysine-arginine-lysine (CGKRK) were fabricated for tumor-targeted imaging and employed multiple modalities to track the accumulation of the probes in tumors.…”

Section: In Vivo Animal Imagingmentioning

confidence: 99%

Quantum Dots: A Review from Concept to Clinic

Kargozar

Hoseini

Milan

et al. 2020

Biotechnology Journal

148

101

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Quantum dots (QDs) are semiconductor materials that have gained great interest due to their unique characteristics like optical properties. They are extensively being used in different areas, including solar cells, light-emitting diodes, laser technology, as well as biological and biomedical applications. In this review, comprehensive information about different aspects of QDs is provided, including their types and classifications, synthesis approaches, in vitro and in vivo toxicity, biological applications, and potentials in clinical applications. With a focus on the biological aspects, the respective in vitro and in vivo studies are collected and presented. Various surface modifications on QDs are discussed as directly influencing their properties like toxicity and optical abilities. Given the promising results, these materials are clinically used for targeted molecular therapy and imaging. However, there are a large number of questions that should be addressed before the wide application of QDs in a clinical setting. Regarding the existing barriers to QDs, suggestions are given and discussed to present an appropriate route for the clinical use of these materials.

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Targeting and imaging of monocyte-derived macrophages in rat's injured artery following local delivery of liposomal quantum dots

Cited by 14 publications

References 65 publications

Orange-Emitting ZnSe:Mn²⁺ Quantum Dots as Nanoprobes for Macrophages

Orange-Emitting ZnSe:Mn²⁺ Quantum Dots as Nanoprobes for Macrophages

Advances in nanomaterial-based targeted drug delivery systems

Quantum Dots: A Review from Concept to Clinic

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Targeting and imaging of monocyte-derived macrophages in rat's injured artery following local delivery of liposomal quantum dots

Cited by 14 publications

References 65 publications

Orange-Emitting ZnSe:Mn2+ Quantum Dots as Nanoprobes for Macrophages

Orange-Emitting ZnSe:Mn2+ Quantum Dots as Nanoprobes for Macrophages

Advances in nanomaterial-based targeted drug delivery systems

Quantum Dots: A Review from Concept to Clinic

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Product

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Orange-Emitting ZnSe:Mn²⁺ Quantum Dots as Nanoprobes for Macrophages

Orange-Emitting ZnSe:Mn²⁺ Quantum Dots as Nanoprobes for Macrophages