Targeted Protein Profiling of In Vivo NIPP-Treated Tissues Using DigiWest Technology

Abstract

Non-invasive physical plasma (NIPP) is a novel therapeutic tool, currently being evaluated for the treatment of cancer and precancerous lesions in gynecology and other disciplines. Additionally, patients with cervical intraepithelial neoplasia (CIN) may benefit from NIPP treatment due to its non-invasive, side-effect-free, and tissue-sparing character. However, the molecular impact of in vivo NIPP treatment needs to be further investigated. For this purpose, usually only very small tissue biopsies are available after NIPP treatment. Here, we adapted DigiWest technology, a high-throughput bead-based Western blot, for the analysis of formalin-fixed paraffin-embedded (FFPE) cervical punch biopsies with a minimal sample amount. We investigated the molecular effects of NIPP treatment directly after (0 h) and 24 h after in vivo application. Results were compared to in vitro NIPP-treated human malignant cervical cells. NIPP effects were primarily based on an inhibitory impact on the cell cycle and cell growth factors. DigiWest technology was suitable for detailed protein profiling of small, primary FFPE biopsies.

Plasma Device Functions and Tissue Effects in the Female Pelvis—A Systematic Review

“…Definite proof of treatment selectiveness remains scarce, as culture media used for cancerous and non-cancerous cell lines often differ, which can affect the stability of reactive oxygen and nitrogen species [ 89 ]. Additionally, it is uncertain whether changes in protein expression realised in cell cultures can be transferred to in vivo treatment [ 90 ]. Finally, the CAP-affected zone needs to be better understood, and methods for local CAP delivery may need to be developed.…”

A composition-tunable cold atmospheric plasma chip for multiplex-treatment of cells

“…Treatment results on cellular spheroids or even organoids can provide more representative guidelines for the clinical application, due to the more accurate tumor environment simulation. 64 Tissue exposure can help evaluate the damage of plasma treatment at an ex vivo level and explore the penetration depth of the different plasma compositions. [64][65][66]…”

“…64 Tissue exposure can help evaluate the damage of plasma treatment at an ex vivo level and explore the penetration depth of the different plasma compositions. [64][65][66]…”

Cold Argon Athmospheric Plasma for Biomedicine: Biological Effects, Applications and Possibilities

“…The authors used marker-independent Raman spectroscopy to determine the functional tissue penetration depth of direct plasma treatment. A working group at the NMI (Natural and Medical Science Institute) published data comparing the analysis of protein expression using Digi-West technology following plasma treatment of 2D single cells and 3D tissue [ 85 ]. This study shows the importance of investigating plasma effects on 3D tissue due to significantly differing results compared to single cell studies, which so far represent the (incomplete) data basis of our understanding of plasma effects.…”