2011
DOI: 10.1586/epr.11.49
|View full text |Cite
|
Sign up to set email alerts
|

Targeted protein-omic methods are bridging the gap between proteomic and hypothesis-driven protein analysis approaches

Abstract: While proteomic methods have illuminated many areas of biological protein space, many fundamental questions remain with regard to systems-level relationships between mRNAs, proteins, and cell behaviors. While mass spectrometric methods offer a panoramic picture of the relative expression and modification of large numbers of proteins, they are neither optimal for the analysis of pre-defined targets across large numbers of samples nor for assessing differences in proteins between individual cells or cell compart… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
15
0

Year Published

2012
2012
2017
2017

Publication Types

Select...
6
1
1

Relationship

2
6

Authors

Journals

citations
Cited by 21 publications
(15 citation statements)
references
References 78 publications
0
15
0
Order By: Relevance
“…To determine the global short-term effects of CAPE on the changes in cellular signaling proteins related to Akt signaling, cell stress, and cell proliferation in LNCaP 104-S cells, we performed Micro-Western Array analysis (MWA) (21, 22, 30, 31) of 28 protein abundances and modifications in LNCaP 104-S cells at six time points from 0 to 480 min following replenishment of serum- and androgen-containing medium in the absence or presence of 10 μM CAPE (Fig. 3; Supp.…”
Section: Resultsmentioning
confidence: 99%
“…To determine the global short-term effects of CAPE on the changes in cellular signaling proteins related to Akt signaling, cell stress, and cell proliferation in LNCaP 104-S cells, we performed Micro-Western Array analysis (MWA) (21, 22, 30, 31) of 28 protein abundances and modifications in LNCaP 104-S cells at six time points from 0 to 480 min following replenishment of serum- and androgen-containing medium in the absence or presence of 10 μM CAPE (Fig. 3; Supp.…”
Section: Resultsmentioning
confidence: 99%
“…Antibodies are meant to recognize an epitope on target molecules; so off target cross-reactivity can badly affect quantitative results. Therefore, multiple validation and nor-malization steps are necessary to eliminate false positive signals in antibody-based proteomics [9,10]. On the other hand, modular domains naturally have wide-ranging specificity; promiscuity in ligand selection is considered a physiological propensity rather than experimental noise.…”
Section: Modular Protein Interacting Domain As Affinity Reagentsmentioning
confidence: 99%
“…Micro-Western Arrays (MWAs) represent a new method for proteomic analyses [19]. The MWA is a modification of the antibody-based modified reverse phase protein array.…”
Section: Discussionmentioning
confidence: 99%