Targeted production of desired minor ginsenosides based on the hydrolytic selectivity of β-glucosidase and their enhanced anti-neuroinflammatory activity

Kim, Young Soo; Oh, You-Chang

doi:10.1016/j.procbio.2019.06.028

Cited by 1 publication

(1 citation statement)

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“…Ginsenosides are classified into three groups based on the types of aglycone structures; namely, oleanane (Ro), protopanaxadiol-type (Rb 1 , Rb 2 , Rb 3 , Rc, Rd, F 2 , Compound K, and PPD), and protopanaxatriol-type (Re, Rf, Rg 1 , Rg 2 , Rh 1, and PPT) [5]. MGs [6,7] are more pharmacologically active due to their lower molecular weight, higher cell membrane permeability, and easier absorption in the gastrointestinal tract compared to the major ginsenosides [8]. Recent in vivo and in vitro studies have shown that MGs have many pharmacological activities, including anti-tumour [9], anti-inflammation [10,11], anti-cancer [12,13], skincare [14], and hypoglycaemic [15] properties.…”

Section: Introductionmentioning

confidence: 99%

Production of minor ginsenosides by combining Stereum hirsutum and cellulase

et al. 2021

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Minor ginsenosides (MGs) (include ginsenoside F2, Compound K, PPT, etc), which are generally not produced by ginseng plants naturally, are obtained by deglycosylation of major ginsenosides. However, the conventional processes used to produce deglycosylated ginsenosides focus on the use of intestinal microorganisms for transformation. In this study, an edible and medicinal mushroom Stereum hirsutum JE0512 was screened from 161 β-glucosidase-producing soil microorganisms sourced from wild ginseng using the plate coloration method. Furthermore, JE0512 was used for the production of CK from ginseng extracts (GE) in solid-state fermentation (SSF) using 20 g corn bran as substrate, 4 g GE, and 20% inoculation volume, and the results showed that the highest CK content was 29.13 mg/g. After combining S. hirsutum JE0512 with cellulase (Aspergillus niger), the MGs (F2, CK, and PPT) content increased from 1.66 to 130.79 mg/g in the final products. Our results indicate that the Stereum genus has the potential to biotransform GE into CK and the combination of S. hirsutum JE0512 and cellulase could pave the way for the production of MGs from GE.

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