Targeted nuclear irradiation with a proton microbeam induces oxidative DNA base damage and triggers the recruitment of DNA glycosylases OGG1 and NTH1

Robeska, Elena; Lalanne, Kévin; Vianna, François; Sutcu, Haser Hasan; Khobta, Andriy; Busso, Didier; Radicella, J. Pablo; Campalans, Anna; Baldeyron, Céline

doi:10.1016/j.dnarep.2023.103610

Cited by 2 publications

(2 citation statements)

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“…To assess initial IR-induced DNA damage response of myogenic cells, we used the immortalized murine C2C7 cells stably expressing GFP-tagged repair proteins and the microbeam MIRCOM facility, which allows in situ localized irradiation within the nuclei of living cells (33,39,43). The MIRCOM microbeam can deliver a controlled number of a given particle.…”

Section: The Dynamic Behavior Of Ku80-gfp At the Dna Damage Site Depe...mentioning

confidence: 99%

The DNA damage response relies on the characteristics of ionizing particles in myogenic cells

Sutcu,

Thomas--Joyeux,

Cardot-Martin

et al. 2024

Preprint

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DNA integrity and stability are vital for proper cellular activity. Nevertheless, to treat cancer patients, DNA is the main target for inducing tumoral cell death. Nowadays, cancer treatment is improving by the development of new technologies, protocols and strategies. Amongst them, the introduction of charged particles as a form of radiotherapy is underway. However, tumor-neighboring healthy tissues are still exposed to ionizing radiations (IR) and subject to late side effects. Skeletal muscle is one of those tissues most likely to be affected. To decipher the DNA damage response (DDR) of skeletal muscle cells, myogenic cells, we irradiated them with microbeams of protons or α-particles and followed the accumulation of DDR proteins at irradiation sites. Thereby, we showed that myoblasts, proliferating myogenic cells, repair IR-induced DNA damage through both non-homologous end-joining and homologous recombination with different recruitment dynamics depending on the characteristics of ionizing particles (type, energy deposition and time after irradiation), whereas myotubes, post-mitotic myogenic cells, display reduced DNA damage response.SummaryMouse skeletal muscle cells and their precursor cells irradiated by ion microbeam show DNA damage response and DNA repair dynamics that vary according to the characteristics of ionizing particles delivered.Graphical Abstract

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Section: The Dynamic Behavior Of Ku80-gfp At the Dna Damage Site Depe...mentioning

confidence: 99%

The DNA damage response relies on the characteristics of ionizing particles in myogenic cells

Sutcu,

Thomas--Joyeux,

Cardot-Martin

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Radiation sites induce the oxidation of bases such as 7, 8-dihydro-8-oxyguanine (8-oxoG) and thymine glycol (TG). When cells initiate the BER pathway, DNA glycosylases OGG1 and NTH1, which cleave 8-oxoG and TG respectively, are recruited to the damaged site ( Robeska et al, 2024 ). Once the initial oxidative damaged base is cleaved by DNA glycosylase, apurinic/apyrimidinic (AP) sites are produced.…”

Section: Key Atr Signaling Events Under Irradiationmentioning

confidence: 99%