Targeted multicolor in vivo imaging over 1,000 nm enabled by nonamethine cyanines

Bandi, Venugopal; Luciano, Michael; Saccomano, Mara; Patel, Nimit L.; Bischof, Thomas; Lingg, Jakob G. P.; Tsrunchev, Peter; Nix, Meredith N.; Ruehle, Bastian; Sanders, Chelsea; Riffle, Lisa; Robinson, Christina M.; Difilippantonio, Simone; Kalen, Joseph D.; Resch‐Genger, Ute; Ivanic, Joseph; Bruns, Oliver T.; Schnermann, Martin J.

doi:10.1038/s41592-022-01394-6

Cited by 91 publications

(97 citation statements)

References 55 publications

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“…In FRET and secondary absorption systems, molecular dyes absorb and quench the emission light of inorganic emitters to quench the fluorescence signal or influence luminescence lifetime (MY-1057, 49 Flav7, 53 5H5, 54 CX-3, 55 BTC1070, 56 Rh-1029, 57 VIX-4, 58 NIRII-HD5, 59 LET-1052, 51 NRh, 42 NIR-1380, 60 and FNIR-1072. 61 ). However, in dye sensitizing and ACIE systems, molecular dyes absorb excitation light, and then either transfer energy to emitters (in a dye sensitizing system, such as ICG, 62 ALK-pi, 63 and NPh 64 ) or quench the emitters (in ACIE systems, such as IR-808, 65 and NPTAT 66 ).…”

Section: Construction Of Nir-ii Organic/inorganic Nanohybridsmentioning

confidence: 99%

Management of fluorescent organic/inorganic nanohybrids for biomedical applications in the NIR-II region

Zhao

Lin

et al. 2022

Chem. Soc. Rev.

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Section: Construction Of Nir-ii Organic/inorganic Nanohybridsmentioning

confidence: 99%

Management of fluorescent organic/inorganic nanohybrids for biomedical applications in the NIR-II region

Zhao

Lin

et al. 2022

Chem. Soc. Rev.

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“…We recently demonstrated that dichloro-substituted nonamethine cyanine derivatives could be modified with either catechols or through direct aryl fusion to generate FNIR-866 and FNIR-1072, respectively ( Figure 3 b). These probes can be used as mAb-targeted agents for multicolor surgical applications [ 142 ]. Overall, the rapid progress in the field of long-wavelength probe chemistry is opening up a range of exciting possibilities.…”

Section: Recent Developments In Nir and Swir Fluorophoresmentioning

confidence: 99%

Targeted Dual-Modal PET/SPECT-NIR Imaging: From Building Blocks and Construction Strategies to Applications

Usama

Marker

Vargas

et al. 2022

Cancers

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Molecular imaging is an emerging non-invasive method to qualitatively and quantitively visualize and characterize biological processes. Among the imaging modalities, PET/SPECT and near-infrared (NIR) imaging provide synergistic properties that result in deep tissue penetration and up to cell-level resolution. Dual-modal PET/SPECT-NIR agents are commonly combined with a targeting ligand (e.g., antibody or small molecule) to engage biomolecules overexpressed in cancer, thereby enabling selective multimodal visualization of primary and metastatic tumors. The use of such agents for (i) preoperative patient selection and surgical planning and (ii) intraoperative FGS could improve surgical workflow and patient outcomes. However, the development of targeted dual-modal agents is a chemical challenge and a topic of ongoing research. In this review, we define key design considerations of targeted dual-modal imaging from a topological perspective, list targeted dual-modal probes disclosed in the last decade, review recent progress in the field of NIR fluorescent probe development, and highlight future directions in this rapidly developing field.

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“…The current array of preclinically validated SWIR targeting agents will still require further validation and toxicology studies before reaching clinical deployment. (36,40,42) Clinical SWIR translation has predominantly focused on non-targeted ICG based imaging and has already shown significant improvements over NIR imaging for liver tumor surgery, glioma resection, cystic renal mass removal and brain metastasis. (43)(44)(45)(46) Fortunately, pHLIP ICG (pH low insertion peptide, conjugated to ICG), which until this point has not been validated with SWIR imaging, is a tumor targeting agent that is currently undergoing clinical trials for imaging-guided breast cancer resection (NCT05130801).…”

Section: Introductionmentioning

confidence: 99%

“…( 39 ) In addition to ICG imaging there has been a rapid development of dedicated SWIR fluorescence agents for in vivo applications. ( 40, 41 ) One area which would greatly benefit from SWIR imaging is that of clinical cancer resection. In comparison to functional imaging of e.g., calcium dynamics, cancer resection provides a more facile translation for SWIR imaging where imaging can be performed with a binary output of tumor/no tumor for resection if a dye can be targeted and localized to a tumor.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Preclinical shortwave infrared tumor screening and resection via pHLIP ICG under ambient lighting conditions

Larney

Kim

Roberts

et al. 2022

Preprint

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There is a critical need to improve optical imaging that will lead to its widespread acceptance for routine clinical procedures. Shortwave infrared (SWIR, 900 to 1700nm) imaging has demonstrated clear advantages over visible and near infrared imaging (reduced autofluorescence with improved contrast, resolution, and sensitivity at tissue depth). Here we show that the previously reported compound, pH low insertion peptide (pHLIP) conjugated to indocyanine green (ICG, pHLIP ICG) currently in clinical trials, serves as an excellent candidate for SWIR imaging protocols. SWIRs increased sensitivity enabled preclinical tumor screening and resection at exposure times as low as 0.1 ms with acceptable signal to noise and contrast to noise ratios. Imaging was performed under ambient lighting conditions, and SWIRs sensitivity enabled an extended surgical resection window up to 96 hrs post injection in an orthotopic breast cancer mouse model. This work provides a direct precedent for the clinical translation of SWIR pHLIP ICG imaging for cancer resection.

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Targeted multicolor in vivo imaging over 1,000 nm enabled by nonamethine cyanines

Cited by 91 publications

References 55 publications

Management of fluorescent organic/inorganic nanohybrids for biomedical applications in the NIR-II region

Management of fluorescent organic/inorganic nanohybrids for biomedical applications in the NIR-II region

Targeted Dual-Modal PET/SPECT-NIR Imaging: From Building Blocks and Construction Strategies to Applications

Preclinical shortwave infrared tumor screening and resection via pHLIP ICG under ambient lighting conditions

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