Targeted metabolite profiling to evaluate unintended metabolic changes of genetic modification in resveratrol-enriched rice (Oryza sativa L.)

Kim, Min Sung; Baek, Seong-Bum; Park, Sang Un; Im, Kyung˗Hoan; Kim, Jae Kwang

doi:10.1007/s13765-017-0265-0

Cited by 17 publications

(11 citation statements)

References 24 publications

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“…With regard to metabolic variations, environmental factors, e.g., growing season, location, sowing date, and type of abiotic stress, play a larger role than genetic modifications [26][27][28]. Because those factors may be the dominant parameter driving the variability of metabolite profiles in the PCA, we also conducted a comparative analysis to examine individual differences in the datasets of transgenic soybean versus its non-transgenic counterpart.…”

Section: Discussionmentioning

confidence: 99%

Global metabolite profiling based on GC–MS and LC–MS/MS analyses in ABF3-overexpressing soybean with enhanced drought tolerance

Nam

Kim

et al. 2019

Appl Biol Chem

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Abscisic acid (ABA) is a phytohormone that plays an important role in the adaptive responses to abiotic stresses. We examined the metabolic changes in transgenic soybean that over-expressed Arabidopsis ABA responsive elementbinding factor 3 (ABF3), which participates in drought tolerance. Transgenic and non-transgenic plants were exposed to a water deficit, and their metabolic differences were verified by untargeted GC-MS and LC-MS/MS analyses. A total of 64 and 476 primary and secondary metabolites from leaf extracts were identified based on GC-MS and LC-MS/MS platforms, respectively. Principal component analysis derived from both GC-MS and LC-MS/MS data showed a clearly greater separation in the metabolite profiles among three different degrees of drought stress. However, no discrimination of metabolites between transgenic and non-transgenic plants was apparent. Furthermore, except for some free amino acids, quantitative differences in relative levels of those metabolites were less than 50% between genotypes. These results suggest that, during periods of drought, overexpression of ABF3 in transgenic soybean might result in a negligible variance in primary and secondary metabolism when compared with its non-transgenic counterpart.

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Section: Discussionmentioning

confidence: 99%

Global metabolite profiling based on GC–MS and LC–MS/MS analyses in ABF3-overexpressing soybean with enhanced drought tolerance

Nam

Kim

et al. 2019

Appl Biol Chem

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“…Inferential tests of differences (independent t-tests) and relationships (Pearson's coefficient) were performed using SAS software (version 9.2: SAS Institute, Cary, NC, USA). The relative quantification data acquired from GC-TOFMS were subjected to principal component analysis (PCA) using SIMCA-P software (version 13.0; Umetrics, Umea, Sweden) to evaluate similarity or dissimilarity between groups of multivariate data [12]. The PCA output consisted of score plots for visualizing the contrast between different samples and loading plots to explain cluster separation.…”

Section: Discussionmentioning

confidence: 99%

Responses of Primary Metabolites and Glucosinolates in Sulfur Deficient-Cabbage (Brassica rapa L. ssp. Pekinensis)

Sung¹,

Baek²,

Kim³

et al. 2018

J Plant Biochem Physiol

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Sulfur (S) is an essential mineral nutrient for plant growth and development and is a key component of many biological compounds. As S acquisition and assimilation have important roles in plant metabolism, S-deficient responses are closely involved in different plant constituents. In this study, we examined the effects of S deficiency on primary metabolism and glucosinolate (GSL) content in cabbage (Brassica rapa) plants. Soluble sugars such as glucose, fructose, galactose, and xylose, were up to 0.19-fold lower under S deficiency, and these changes were more pronounced with long-term (15 d) S deficiency. Significant increases in amino acids were observed in terms of glutamine (6.35-fold), glycine (20.54), serine (3.56), threonine (3.25), phenylalanine (4.07), β-alanine (7.88), and proline (4.58). S deficiency led to large accumulation of an indolyl GSL, 4-methoxyglucobrassicin, in both shoots (2.68 fold) and roots (5.99 fold). GSLs were positively correlated with the majority of primary metabolites in the shoots, but negative in the roots. Thus, at least in cabbage plants, the interplay between primary metabolism and GSLs appeared to be tissue-dependent, and the metabolic interaction between both metabolites should be elucidated.

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“…All analyses were performed at least three times. Principal component analysis (PCA; SIMCA-P version 13.0; Umetrics, Umeå, Sweden) was conducted using the mineral element data and the metabolite data as in Kim et al [9]. The analysis was set to produce score plots to illustrate the dissimilarity between samples treated with different nutrient deficiencies and loading plots to show mineral elements and metabolites explaining the cluster separation.…”

Section: Discussionmentioning

confidence: 99%

“…Primary metabolites were extracted as described by Kim et al [9]. Powdered sample that was freeze-dried and ground (100 mg) was mixed with 1 mL of a mixture containing methanol, water, and chloroform (2.5:1:1 volume ratio) and 60 lL of ribitol as an internal standard (0.2 mg/mL) at 37°C for 30 min with a mixing frequency of 1200 rpm using a Thermomixer Compact (Eppendorf AG, Hamburg, Germany).…”

Section: Metabolic Profilingmentioning

confidence: 99%

Metabolite profiling and mineral nutrient analysis from the leaves and roots of bell pepper (Capsicum annuum L. var. angulosum) grown under macronutrient mineral deficiency

Kim

Lee

et al. 2018

Appl Biol Chem

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We analyzed the contents of 38 primary metabolites and 9 minerals in the leaves and roots of bell pepper (Capsicum annuum L. var. angulosum) to study metabolic responses to deficiency in nitrogen, phosphorus, potassium, calcium, or magnesium. Induced deficiencies of individual cations reduced the abundance of the other cations in both leaves and roots. Each nutrient-deficient condition was clearly grouped by principal component analysis, which also showed that leaves under cation-deficiency treatments were separated from those under noncation-deficiency treatments. This was consistent with that a single cation deficiency decreased the levels of the other cations in leaves. Specifically, N deficiency reduced amino acids and organic acids in both tissues. The common response to P-, K-, Ca-or Mg-deficient conditions showed significant increases in the levels of amino acids in both tissues and organic acids in the roots. In the leaves, P-or Mg-deficient conditions reduced organic acids. Soluble carbohydrates were significantly increased under N-, K-, Ca-or Mg-deficient conditions in the leaves, whereas in roots under K deficiency. Notably, the level of caminobutyric acid, an amino acid that helps protect against biotic and abiotic stresses, was increased threefold in leaves under K-deficient conditions and sixfold in roots under P-, K-, Ca-, or Mg-deficient conditions. These findings provide additional information about variations in metabolite and mineral abundance in bell pepper leaves and roots in response to mineral shortage.

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Targeted metabolite profiling to evaluate unintended metabolic changes of genetic modification in resveratrol-enriched rice (Oryza sativa L.)

Cited by 17 publications

References 24 publications

Global metabolite profiling based on GC–MS and LC–MS/MS analyses in ABF3-overexpressing soybean with enhanced drought tolerance

Global metabolite profiling based on GC–MS and LC–MS/MS analyses in ABF3-overexpressing soybean with enhanced drought tolerance

Responses of Primary Metabolites and Glucosinolates in Sulfur Deficient-Cabbage (Brassica rapa L. ssp. Pekinensis)

Metabolite profiling and mineral nutrient analysis from the leaves and roots of bell pepper (Capsicum annuum L. var. angulosum) grown under macronutrient mineral deficiency

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