Global metabolite profiling based on GC–MS and LC–MS/MS analyses in ABF3-overexpressing soybean with enhanced drought tolerance

Nam, Kyong‐Hee; Kim, Do Young; Kim, Hye Jin; Pack, In-Soon; Kim, Hye Jeong; Chung, Young Soo; Kim, Soo Young; Kim, Chang-Gi

doi:10.1186/s13765-019-0425-5

Cited by 30 publications

(21 citation statements)

References 37 publications

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“…Osmotic stress activates ABA biosynthesis and inhibits ABA degradation (Fernando and Schroeder, 2016;Nam et al, 2019;Seo et al, 2019). Furthermore, it is known that the Salt Overly Sensitive (SOS) signaling pathway plays an important role in salt stress tolerance and ion homeostasis (Zhu, 2016).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of the HDA15 Gene Confers Resistance to Salt Stress by the Induction of NCED3, an ABA Biosynthesis Enzyme

et al. 2021

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Salt stress constitutes a major form of abiotic stress in plants. Histone modification plays an important role in stress tolerance, with particular reference to salt stress resistance. In the current study, we found that HDA15 overexpression confers salt stress resistance to young seedling stages of transgenic plants. Furthermore, salt stress induces HDA15 overexpression. Transcription levels of stress-responsive genes were increased in transgenic plants overexpressing HDA15 (HDA15 OE). NCED3, an abscisic acid (ABA) biosynthetic gene, which is highly upregulated in HDA15 transgenic plants, enhanced the accumulation of ABA, which promotes adaptation to salt stress. ABA homeostasis in HDA15 OE plants is maintained by the induction of CYP707As, which optimize endogenous ABA levels. Lastly, we found that the double-mutant HDA15 OE/hy5 ko plants are sensitive to salt stress, indicating that interaction between HDA15 and ELONGATED HYPOCOTYL 5 (HY5) is crucial to salt stress tolerance shown by HDA15 OE plants. Thus, our findings indicate that HDA15 is crucial to salt stress tolerance in Arabidopsis.

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Section: Introductionmentioning

confidence: 99%

Overexpression of the HDA15 Gene Confers Resistance to Salt Stress by the Induction of NCED3, an ABA Biosynthesis Enzyme

et al. 2021

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“…Although cultivation of transgenic crops is not yet allowed in South Korea, large amounts of transgenic crops are imported for food and feed; in 2018, South Korea imported 1.0 million tons of transgenic soybeans [4]. In addition, various transgenic soybeans have been developed and their performance has been tested in experimental fields in South Korea [5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Gene flow from herbicide resistant transgenic soybean to conventional soybean and wild soybean

Kim

Moon

et al. 2019

Appl Biol Chem

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Gene flow from transgenic crops to conventional cultivars or wild relatives is a major environmental and economic concern in many countries. South Korea is one of the major importer of transgenic crops for food and feed, although commercial cultivation of transgenic crops is not yet allowed in this country. This study evaluated gene flow from the herbicide glyphosate-and glufosinate-resistant transgenic soybean (Glycine max) to five non-transgenic soybean cultivars and three accessions of wild soybean (Glycine soja). Field trials were conducted over 2 years, and gene flow was monitored up to 10 m distance from the pollen source. The results indicated that the detectable rate of gene flow from transgenic to conventional soybeans varied between 0 and 0.049% in both 2014 and 2015 field trials, while no hybrids were detected among wild soybean progenies. The highest rate of gene flow was found in the progenies of the Bert cultivar, which exhibited the longest period of flowering synchronization between the pollen donor and the recipient. In addition, overall gene flow rates declined with increased distance from the transgenic soybean plot. Gene flow was observed up to 3 m and 8 m from the transgenic soybean plot in 2014 and 2015, respectively. Our results may be useful for developing measures to prevent gene flow from transgenic soybean. which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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“…Processed binning results were imported to SIMCA 15.0.2, and the principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA), and orthogonal partial least squares discriminant analysis (OPLS-DA) were analyzed. PCA is an unsupervised method for checking the distribution of samples [13]. In the PCA score plot, WG and BG were clearly separated from each other and from TG and RG.…”

Section: Resultsmentioning

confidence: 99%

A Comparative Study on Processed Panax ginseng Products Using HR-MAS NMR-Based Metabolomics

et al. 2020

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Panax ginseng is processed to diversify efficacy. Four processed ginsengs containing white ginseng (WG), tae-geuk ginseng (TG), red ginseng (RG), and black ginseng (BG) were analyzed using nuclear magnetic resonance (NMR) spectroscopy for screening overall primary metabolites. There were significant differences in the sugar content among these four processed ginseng products. WG had a high sucrose content, TG had a high maltose content, and BG had high fructose and glucose content. In the multivariate analyses of NMR spectra, the PCA score plot showed significant discrimination between the four processed ginsengs. For effective clustering, orthogonal partial least squares discriminant analyses (OPLS-DA) with a 1:1 comparison were conducted and all OPLS models were validated using the permutation test, the root mean square error of estimation (RMSEE), and the root mean square error of prediction (RMSEP). All OPLS-DA score plots showed clear separations of processed ginseng products, and sugars such as sucrose and fructose mainly contributed to these separations.

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Global metabolite profiling based on GC–MS and LC–MS/MS analyses in ABF3-overexpressing soybean with enhanced drought tolerance

Cited by 30 publications

References 37 publications

Overexpression of the HDA15 Gene Confers Resistance to Salt Stress by the Induction of NCED3, an ABA Biosynthesis Enzyme

Overexpression of the HDA15 Gene Confers Resistance to Salt Stress by the Induction of NCED3, an ABA Biosynthesis Enzyme

Gene flow from herbicide resistant transgenic soybean to conventional soybean and wild soybean

A Comparative Study on Processed Panax ginseng Products Using HR-MAS NMR-Based Metabolomics

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