Targeted Knockdown of Insulin-Like Growth Factor Binding Protein-2 Disrupts Cardiovascular Development in Zebrafish Embryos

Wood, Adrienne; Schlueter, Peter J.; Duan, Cunming

doi:10.1210/me.2004-0392

Cited by 69 publications

(46 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…IGFBP2 Knockdown of IGFBP2 resulted in impaired vessel sprouting in zebrafish embryos (Wood et al 2005). IGFBP2 increased angiogenesis via transcriptional regulation of VEGF (Azar et al 2011).…”

Section: Insulin-like Growth Factor-binding Proteinsmentioning

confidence: 99%

Endothelial cells and the IGF system

Bach¹

2014

Journal of Molecular Endocrinology

153

118

View full text Add to dashboard Cite

Endothelial cells line blood vessels and modulate vascular tone, thrombosis, inflammatory responses and new vessel formation. They are implicated in many disease processes including atherosclerosis and cancer. IGFs play a significant role in the physiology of endothelial cells by promoting migration, tube formation and production of the vasodilator nitric oxide. These actions are mediated by the IGF1 and IGF2/mannose 6-phosphate receptors and are modulated by a family of high-affinity IGF binding proteins. IGFs also increase the number and function of endothelial progenitor cells, which may contribute to protection from atherosclerosis. IGFs promote angiogenesis, and dysregulation of the IGF system may contribute to this process in cancer and eye diseases including retinopathy of prematurity and diabetic retinopathy. In some situations, IGF deficiency appears to contribute to endothelial dysfunction, whereas IGF may be deleterious in others. These differences may be due to tissue-specific endothelial cell phenotypes or IGFs having distinct roles in different phases of vascular disease. Further studies are therefore required to delineate the therapeutic potential of IGF system modulation in pathogenic processes.

show abstract

Section: Insulin-like Growth Factor-binding Proteinsmentioning

confidence: 99%

Endothelial cells and the IGF system

Bach¹

2014

Journal of Molecular Endocrinology

153

118

View full text Add to dashboard Cite

show abstract

“…Studies on Igfbp2 in fish have pointed out that this binding protein is a growth inhibitory protein, regulating Igf1 bioavailability (Duan et al 1999, Wood et al 2005). Few reports have described the dynamic of muscle-derived igfbp2.…”

Section: Transcriptional Regulation Of Igfbps In the Skeletal Muscle mentioning

confidence: 99%

Dynamic transcriptional regulation of autocrine/paracrine igfbp1, 2, 3, 4, 5, and 6 in the skeletal muscle of the fine flounder during different nutritional statuses

Safian

Fuentes

Valdés³

et al. 2012

Journal of Endocrinology

View full text Add to dashboard Cite

The IGF-binding proteins (IGFBPs) play a dual role in the regulation of the activity and bioavailability of IGFs in different tissues. Diverse evidence has shown that IGFBPs can inhibit and/or potentiate IGF actions. In this study, igfbp1, 2, 3, 4, 5, and 6 were isolated in the fine flounder, a flat fish species that shows slow growth and inherent Gh resistance in muscle. Subsequently, the expression of all igfbps was assessed in the skeletal muscle of flounder that underwent different nutritional statuses. igfbp1 was not expressed in muscle during any of the nutritional conditions, whereas igfbp3 and igfbp5 were the lowest and the highest igfbps expressed respectively. A dynamic expression pattern was found in all the igfbps expressed in skeletal muscle, which depended on the nutritional status and sampling period. During the fasting period, igfbp2, 4, and 5 were downregulated, whereas igfbp3 was upregulated during part of the fasting period. The restoration of food modulated the expression of the igfbps dynamically, showing significant changes during both the long-and short-term refeeding. igfbp3 and igfbp6 were downregulated during short-term refeeding, whereas igfbp5 was upregulated, and igfbp2 and igfbp4 remained stable. During long-term refeeding, the expression of igfbp2, 4, 5, and 6 increased, while igfbp3 remained unchanged. In conclusion, this study shows for the first time the isolation of all igfbps in a single fish species, in addition to describing a dynamic nutritional and time-dependent response in the expression of igfbps in the skeletal muscle of a nonmammalian species.

show abstract

“…In contrast, overexpression of the IGF-1R in mice induces cardiac hypertrophy (McMullen et al, 2004). In zebrafish, angiogenesis is compromised in IGFBP-2 morphant embryos (Wood et al, 2005b), while knockdown of igf-1ra and igf-1rb results in retarded heart morphogenesis (Schlueter et al, 2007). In regenerating zebrafish hearts, the level of igf-2b is significantly upregulated (Lien et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Insulin-like growth factor-2 regulates early neural and cardiovascular system development in zebrafish embryos

Hartnett¹,

Glynn²,

Nolan³

et al. 2010

Int. J. Dev. Biol.

View full text Add to dashboard Cite

The insulin-like growth factor (IGF) family is essential for normal embryonic growth and development and it is highly conserved through vertebrate evolution. However, the roles that the individual members of the IGF family play in embryonic development have not been fully elucidated. This study focuses on the role of IGF-2 in zebrafish embryonic development. Two igf-2 genes, igf-2a and igf-2b, are present in the zebrafish genome. Antisense morpholinos were designed to knock down both igf-2 genes. The neural and cardiovascular defects in IGF-2 morphant embryos were then examined further using wholemount in situ hybridisation, TUNEL analysis and O-dianisidine staining. Knockdown of igf-2a or igf-2b resulted in ventralised embryos with reduced growth, reduced eyes, disrupted brain structures and a disrupted cardiovascular system, with igf-2b playing a more significant role in development. During gastrulation, igf-2a and igf-2b are required for development of anterior neural structures and for regulation of genes critical to dorsal-ventral patterning. As development proceeds, igf-2a and igf-2b play antiapoptotic roles. Gene expression analysis demonstrates that igf-2a and igf-2b play overlapping roles in angiogenesis and cardiac outflow tract development. Igf-2b is specifically required for cardiac valve development and cardiac looping. Injection of a dominant negative IGF-1 receptor led to similar defects in angiogenesis and cardiac valve development, indicating IGF-2 signals through this receptor to regulate cardiovascular development. This is the first study describing two functional igf-2 genes in zebrafish. This work demonstrates that igf-2a and igf-2b are critical to neural and cardiovascular development in zebrafish embryos. The finding that igf-2a and igf2b do not act exclusively in a redundant manner may explain why both genes have been stably maintained in the genome.

show abstract

Targeted Knockdown of Insulin-Like Growth Factor Binding Protein-2 Disrupts Cardiovascular Development in Zebrafish Embryos

Cited by 69 publications

References 48 publications

Endothelial cells and the IGF system

Endothelial cells and the IGF system

Dynamic transcriptional regulation of autocrine/paracrine igfbp1, 2, 3, 4, 5, and 6 in the skeletal muscle of the fine flounder during different nutritional statuses

Insulin-like growth factor-2 regulates early neural and cardiovascular system development in zebrafish embryos

Contact Info

Product

Resources

About