Targeted In Vivo Imaging of Microscopic Tumors with Ferritin‐based Nanoprobes Across Biological Barriers

Cao, Chengqi; Wang, Xuxia; Cai, Yong; Sun, Lei; Tian, Lanxiang; Wu, Hao; He, Xiaoyun; Lei, Hao; Liu, Weifeng; Chen, Guanjun; Zhu, Rixiang; Pan, Yongxin

doi:10.1002/adma.201304544

Cited by 88 publications

(85 citation statements)

References 36 publications

(36 reference statements)

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“…10,11 Cancer cells were cultured by using Dulbecco's Modified Eagle's Medium (DMEM, cat 12800-058, Invitrogen) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin solution (cat C0222, Beyotime) at 37°C in 5% CO 2 atmosphere. In the present study, cells were seeded into six-well polystyrene plate, and then, M-HFn nanoparticles were added until the cell attachment rate reaches about 80%, while the control group of cells was incubated in the absence of M-HFn nanoparticles.…”

Section: Uptake Assay In Cancer Cells and Mrimentioning

confidence: 99%

“…38,39 For in vivo tumor imaging, M-HFn nanoparticles can be accumulated in tumor tissues via receptor-mediated pathway and not be cleared quickly. 11 It was also found by acute toxic study on mice that there was no lethal effect with injection of the M-HFn nanoparticles at proposed high dose over 14 days.…”

mentioning

confidence: 94%

“…8 Several studies have demonstrated that without tumor-targeted ligand modification, the HFn shell of M-HFn nanoparticles can be specifically targeted to transferrin receptor 1 (TfR1) overexpressed on various cancer cells, [9][10][11][12][13] which allow efficiently distinguishing cancerous cells from normal cells both in vitro and in vivo. Because of their high transverse relaxivity and good peroxidase-like activity, 11,14 the M-HFn nanoparticles are found to be a very promising contrast agent for the in vivo detection of microscopic (1-2 mm) tumors by MRI and in vitro detection of tumor tissues with a high sensitivity of 98% and specificity of 95%. 10,11 In addition, tumor-targeted arginine-glycine-aspartate peptide can be easily loaded on the HFn shell via genetic means, which make M-HFn nanoparticles specifically bind to α v β 3 integrin implicated in tumor of a variety of cancer types.…”

mentioning

confidence: 99%

“…Because of their high transverse relaxivity and good peroxidase-like activity, 11,14 the M-HFn nanoparticles are found to be a very promising contrast agent for the in vivo detection of microscopic (1-2 mm) tumors by MRI and in vitro detection of tumor tissues with a high sensitivity of 98% and specificity of 95%. 10,11 In addition, tumor-targeted arginine-glycine-aspartate peptide can be easily loaded on the HFn shell via genetic means, which make M-HFn nanoparticles specifically bind to α v β 3 integrin implicated in tumor of a variety of cancer types. 5,15 The nanoscale size plays a key role in avoiding from agglomeration, usage of MRI, clinical diagnosis, and therapeutics.…”

mentioning

confidence: 99%

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Enhanced magnetic resonance imaging and staining of cancer cells using ferrimagnetic H-ferritin nanoparticles with increasing core size

Cai¹,

Cao²,

He³

et al. 2015

IJN

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Purpose This study is to demonstrate the nanoscale size effect of ferrimagnetic H-ferritin (M-HFn) nanoparticles on magnetic properties, relaxivity, enzyme mimetic activities, and application in magnetic resonance imaging (MRI) and immunohistochemical staining of cancer cells. Materials and methods M-HFn nanoparticles with different sizes of magnetite cores in the range of 2.7–5.3 nm were synthesized through loading different amounts of iron into recombinant human H chain ferritin (HFn) shells. Core size, crystallinity, and magnetic properties of those M-HFn nanoparticles were analyzed by transmission electron microscope and low-temperature magnetic measurements. The MDA-MB-231 cancer cells were incubated with synthesized M-HFn nanoparticles for 24 hours in Dulbecco’s Modified Eagle’s Medium. In vitro MRI of cell pellets after M-HFn labeling was performed at 7 T. Iron uptake of cells was analyzed by Prussian blue staining and inductively coupled plasma mass spectrometry. Immunohistochemical staining by using the peroxidase-like activity of M-HFn nanoparticles was carried out on MDA-MB-231 tumor tissue paraffin sections. Results The saturation magnetization ( M s ), relaxivity, and peroxidase-like activity of synthesized M-HFn nanoparticles were monotonously increased with the size of ferrimagnetic cores. The M-HFn nanoparticles with the largest core size of 5.3 nm exhibit the strongest saturation magnetization, the highest peroxidase activity in immunohistochemical staining, and the highest r 2 of 321 mM −1 s −1 , allowing to detect MDA-MB-231 breast cancer cells as low as 10 4 cells mL −1 . Conclusion The magnetic properties, relaxivity, and peroxidase-like activity of M-HFn nanoparticles are size dependent, which indicates that M-HFn nanoparticles with larger magnetite core can significantly enhance performance in MRI and staining of cancer cells.

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Section: Uptake Assay In Cancer Cells and Mrimentioning

confidence: 99%

mentioning

confidence: 94%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Enhanced magnetic resonance imaging and staining of cancer cells using ferrimagnetic H-ferritin nanoparticles with increasing core size

Cai¹,

Cao²,

He³

et al. 2015

IJN

Self Cite

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“…64 In order to detect the pre-angiogenic and brain tumors at the early stages, it is required to fabricate NPs capable of penetrating the defense barriers. Cao et al, 65 synthesized magnetoferritin NPs capable of transport through the endothelium, epithelium and BBB via clathrin-mediated endocytosis and specifically detected the tumor cells which expressed the transferrin receptor 1. Indeed, the magnetoferritin NPs can be a promising candidate to overcome the challenges associated with the NP biodistribution and targeted imaging of brain cancer.…”

Section: Brain Tumorsmentioning

confidence: 99%

Targeted superparamagnetic iron oxide nanoparticles for early detection of cancer: Possibilities and challenges

Bakhtiary

Saei

Hajipour

et al. 2016

Nanomedicine: Nanotechnology, Biology and Medicine

152

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Nanomedicine, the integration of nanotechnological tools in medicine demonstrated promising potential to revolutionize the diagnosis and treatment of various human health conditions. Nanoparticles (NPs) have shown much promise in diagnostics of cancer, especially since they can accommodate targeting molecules on their surface, which search for specific tumor cell receptors upon injection into the blood stream. This concentrates the NPs in the desired tumor location. Furthermore, such receptor-specific targeting may be exploited for detection of potential metastases in an early stage. Some NPs, such as superparamagnetic iron oxide NPs (SPIONs), are also compatible with magnetic resonance imaging (MRI), which makes their clinical translation and application rather easy and accessible for tumor imaging purposes. Furthermore, multifunctional and/or theranostic NPs can be used for simultaneous imaging of cancer and drug delivery. In this review article, we will specifically focus on the application of SPIONs in early detection and imaging of major cancer types.From the Clinical Editor: Super-paramagnetic iron oxide nanoparticles (SPIONs) have been reported by many to be useful as an MRI contrast agent in the detection of tumors. To further enhance the tumor imaging, SPIONs can be coupled with tumor targeting motifs. In this article, the authors performed a comprehensive review on the current status of using targeted SPIONS in tumor detection and also the potential hurdles to overcome. © 2015 Elsevier Inc. All rights reserved. 1 A significant improvement in cancer survival has been noted over the past three decades for most cancer types. This notable and continuous decrement in tumor fatality is related to advanced development in prevention, early diagnosis and therapeutic approaches and decrease in overall prevalence of smoking.1 Early detection of cancer, especially before cancer cells metastasize, is critical for cancer diagnosis, because early stage cancers can be treated more effectively. However, early-stage diagnosis has been difficult to achieve in most cases since clinical symptoms tend to show up at later stages. Therefore, minimally-and non-invasive methods for early detection of cancer are urgently needed in the field. In this regard, many SPION-based systems are under development for more sensitive imaging and earlier detection of tumors. Since non-targeted SPION species cannot readily differentiate cancer tissues from normal tissues, second-generation SPIONs have been developed which are equipped with targeting moieties that can recognize Nanomedicine: Nanotechnology, Biology, and Medicine 12 (2016) 287 -307

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Enhancing the Effect of Pharmacological Ascorbate in Cancer Therapy via Acid‐Triggered Ferritin Nanoparticles

Yang

Chen

et al. 2019

Adv. Biosys.

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The application of ascorbate (vitamin C) for cancer therapy was first proposed in the 1970s and has shown promising results in recent clinical trials. Pharmacological doses of ascorbate selectively induce cell death in different types of cancer cells through the generation of H2O2. However, some cancer cells are resistant to ascorbate. So increasing the sensitivity of resistant cancer cells to ascorbate has attracted considerable attention. Till now, a few attempts in nanomaterials have been made to improve the effect of ascorbate. In this study, a simple ferritin caged copper nanoparticle (Fn‐Cu) significantly improves the susceptibility of ascorbate‐resistant cancer cells to pharmacological ascorbate via selective inhibition of catalase activity in cancer cells, while having negligible cytotoxicity to normal cells. Remarkably, combination of Fn‐Cu with a lower dose of ascorbate significantly inhibits ascorbate‐resistant tumor growth and metastasis in vivo. These data demonstrate Fn‐Cu has the therapeutic potential by enhancing the effect of ascorbate in cancer therapy.

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Targeted In Vivo Imaging of Microscopic Tumors with Ferritin‐based Nanoprobes Across Biological Barriers

Cited by 88 publications

References 36 publications

Enhanced magnetic resonance imaging and staining of cancer cells using ferrimagnetic H-ferritin nanoparticles with increasing core size

Enhanced magnetic resonance imaging and staining of cancer cells using ferrimagnetic H-ferritin nanoparticles with increasing core size

Targeted superparamagnetic iron oxide nanoparticles for early detection of cancer: Possibilities and challenges

Enhancing the Effect of Pharmacological Ascorbate in Cancer Therapy via Acid‐Triggered Ferritin Nanoparticles

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