Targeted Imaging of VCAM-1 mRNA in a Mouse Model of Laser-Induced Choroidal Neovascularization Using Antisense Hairpin-DNA-Functionalized Gold-Nanoparticles

Uddin, Imam; Kilburn, Tyler C.; Yang, Rong; McCollum, Gary W.; Wright, David W.; Penn, John S.

doi:10.1021/acs.molpharmaceut.8b00661

Cited by 12 publications

(13 citation statements)

References 47 publications

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“…AuNPs inhibited the phosphorylation of ERK1/2, Akt, and FAK in HUVECs [ 13 ]. Optical coherence tomography (OCT)-assisted CNV volume measurement at all time points showed a significant reduction in lesion size in the group that received an intravenous injection of gold nanoparticles when compared with controls [ 11 , 14 ]. Most notably, metal nanoparticles with diameters of 20 and 80 nm (0.0065 µg/mL 20 nm AuNPs, 0.4 µg/mL 80 nm AuNPs, 0.0035 µg/mL 20 nm AgNPs, and 0.22 µg/mL 80 nm AgNPs) are toxic to photoreceptor cells in vitro [ 15 ].…”

Section: Retinopathymentioning

confidence: 99%

Pharmacological Role of Functionalized Gold Nanoparticles in Disease Applications

Wang

Hsiao

et al. 2022

Molecules

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Gold has always been regarded as a symbol of nobility, and its shiny golden appearance has always attracted the attention of many people. Gold has good ductility, molecular recognition properties, and good biocompatibility. At present, gold is being used in many fields. When gold particles are as small as several nanometers, their physical and chemical properties vary with their size in nanometers. The surface area of a nano-sized gold surface has a special effect. Therefore, gold nanoparticles can, directly and indirectly, give rise to different biological activities. For example, if the surface of the gold is sulfided. Various substances have a strong chemical reactivity and are easy to combine with sulfhydryl groups; hence, nanogold is often used in biomedical testing, disease diagnosis, and gene detection. Nanogold is easy to bind to proteins, such as antibodies, enzymes, or cytokines. In fact, scientists use nanogold to bind special antibodies, as a tool for targeting cancer cells. Gold nanoparticles are also directly cytotoxic to cancer cells. For diseases caused by inflammation and oxidative damage, gold nanoparticles also have antioxidant and anti-inflammatory effects. Based on these unique properties, gold nanoparticles have become the most widely studied metal nanomaterials. Many recent studies have further demonstrated that gold nanoparticles are beneficial for humans, due to their functional pharmacological properties in a variety of diseases. The content of this review will be the application of gold nanoparticles in treating or diagnosing pressing diseases, such as cancers, retinopathy, neurological diseases, skin disorders, bowel diseases, bone cartilage disorders, cardiovascular diseases, infections, and metabolic syndrome. Gold nanoparticles have shown very obvious therapeutic and application potential.

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Section: Retinopathymentioning

confidence: 99%

Pharmacological Role of Functionalized Gold Nanoparticles in Disease Applications

Wang

Hsiao

et al. 2022

Molecules

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“…Furthermore, their mass production is facilitated by their ease of synthesis and controlled sizes. of utmost importance are the capacity of aunPs to carry nucleic acid payloads and their unique role in PTT (10,11). The current review divided aunPs into 3 groups: Simple drug-carrying aunPs, simple nucleic acid-carrying aunPs and targeted aunPs.…”

Section: Resultsmentioning

confidence: 99%

“…Gold nPs (aunPs) have received increasing attention as drug delivery vehicles for cancer therapeutics as they can be engineered to obviate drug insolubility, carry nucleic acid payloads for gene therapy, target specific tumor cell lines, modulate drug release and amplify photothermal therapy (PTT) (10,11). The development of gene therapy has generated increasing interest in the potential of aunPs to deliver therapeutic nucleic acid payloads (10,11). aunPs have been designed to carry p53, vascular cell adhesion molecule-1 and other mrnas (10,11).…”

Section: Gold Nanoparticle-mediated Delivery Of Paclitaxel and Nucleimentioning

confidence: 99%

“…The development of gene therapy has generated increasing interest in the potential of aunPs to deliver therapeutic nucleic acid payloads (10,11). aunPs have been designed to carry p53, vascular cell adhesion molecule-1 and other mrnas (10,11). Furthermore, poly(thymine)-functionalized aunPs have been synthesized to target mrna translation using a pcdna6 vector expressing a bovine growth hormone polyadenylation signal (12).…”

Section: Gold Nanoparticle-mediated Delivery Of Paclitaxel and Nucleimentioning

confidence: 99%

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Gold nanoparticle‑mediated delivery of paclitaxel and nucleic acids for cancer therapy (Review)

et al. 2020

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Paclitaxel is a potent antineoplastic agent, but poor solubility and resistance have limited its use. Gold nanoparticles (aunPs) are widely studied as drug carriers because they can be engineered to prevent drug insolubility, carry nucleic acid payloads for gene therapy, target specific tumor cell lines, modulate drug release and amplify photothermal therapy. consequently, the conjugation of paclitaxel with aunPs to improve antiproliferative and pro-apoptotic potency may enable improved clinical outcomes. There are currently a number of different aunPs under development, including simple drug or nucleic acid carriers and targeted aunPs that are designed to deliver therapeutic payloads to specific cells. The current study reviewed previous research on aunPs and the development of aunP-based paclitaxel delivery. Contents 1. introduction 2. Mechanisms of paclitaxel action and drug resistance 3. development of aunPs for antineoplastic drug delivery 4. Further innovations 5. limitations 6. conclusion

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“…An in vivo model of human “wet” AMD is laser-induced choroidal neovascularization (mouse LCNV) mouse, in which the inflammatory biomarker vascular cell adhesion molecule-1 (VCAM-1) is highly expressed. Gold nanoparticles functionalized with anti-sense DNA complementary to VCAM-1 mRNA were developed by Uddin et al, who aimed to detect this molecule, thus assessing the occurrence of oxidative stress [ 147 ]. The fluorescence in-situ hybridization (FISH) technique was used to perform photothermal-optical coherence tomography (PT-OCT) involving a fluorescent probe (Alexafluor-647) bonded to 3′ end of anti-sense DNA in order to highlight its interaction with the target mRNA.…”

Section: Fluorescent Nanosystems In Ocular Applicationmentioning

confidence: 99%

Fluorescent Nanosystems for Drug Tracking and Theranostics: Recent Applications in the Ocular Field

et al. 2022

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The greatest challenge associated with topical drug delivery for the treatment of diseases affecting the posterior segment of the eye is to overcome the poor bioavailability of the carried molecules. Nanomedicine offers the possibility to overcome obstacles related to physiological mechanisms and ocular barriers by exploiting different ocular routes. Functionalization of nanosystems by fluorescent probes could be a useful strategy to understand the pathway taken by nanocarriers into the ocular globe and to improve the desired targeting accuracy. The application of fluorescence to decorate nanocarrier surfaces or the encapsulation of fluorophore molecules makes the nanosystems a light probe useful in the landscape of diagnostics and theranostics. In this review, a state of the art on ocular routes of administration is reported, with a focus on pathways undertaken after topical application. Numerous studies are reported in the first section, confirming that the use of fluorescent within nanoparticles is already spread for tracking and biodistribution studies. The first section presents fluorescent molecules used for tracking nanosystems’ cellular internalization and permeation of ocular tissues; discussions on the classification of nanosystems according to their nature (lipid-based, polymer-based, metallic-based and protein-based) follows. The following sections are dedicated to diagnostic and theranostic uses, respectively, which represent an innovation in the ocular field obtained by combining dual goals in a single administration system. For its great potential, this application of fluorescent nanoparticles would experience a great development in the near future. Finally, a brief overview is dedicated to the use of fluorescent markers in clinical trials and the market in the ocular field.

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Targeted Imaging of VCAM-1 mRNA in a Mouse Model of Laser-Induced Choroidal Neovascularization Using Antisense Hairpin-DNA-Functionalized Gold-Nanoparticles

Cited by 12 publications

References 47 publications

Pharmacological Role of Functionalized Gold Nanoparticles in Disease Applications

Pharmacological Role of Functionalized Gold Nanoparticles in Disease Applications

Gold nanoparticle‑mediated delivery of paclitaxel and nucleic acids for cancer therapy (Review)

Fluorescent Nanosystems for Drug Tracking and Theranostics: Recent Applications in the Ocular Field

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