Targeted heat shock protein 72 for pulmonary cytoprotection

Parseghian, Missag H.; Hobson, Stephen; Richieri, Richard A.

doi:10.1111/nyas.13059

Cited by 13 publications

(4 citation statements)

References 60 publications

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“…During tendon damage and collagen synthesis, the cells that have multiple fibripositors and cytoplasmic protrusions can be found in the area [4]. HSP72 is the most important member of the heat-shock protein family [24]. Studies have confirmed that when body cells are subjected to stressful conditions, such as hyperpyrexia, oxidation, and mechanical damage, the increased expression of HSP72 can strengthen the tolerance of cells to damage, improve the viability of the cells, and reduce cell necrosis or apoptosis caused by stress injury [25].…”

Section: Discussionmentioning

confidence: 99%

Thermal Preconditioning May Prevent Tendon Adhesion by Up-Regulating HSP72 in Rats

Tan

et al. 2017

Cell Physiol Biochem

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Background/Aims: The study aims to determine the effects of thermal preconditioning on tendon adhesion by regulating the expression of heat shock protein 72 (HSP72) in rat models. Methods: Sixty male Wistar rats were collected and randomly assigned into the thermal preconditioning and control groups. During the 4^th and 8^th weeks following surgery, 15 rats were sacrificed in each period respectively, and their tendon adhesion was observed and evaluated. Biomechanical testing was performed to measure the tensile strength and gliding distance of tendons. Hematoxylin-eosin (HE) was used to observe the morphological structure of the tendons. Immunohistochemical staining, quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting were used to detect the HSP72, fibroblast growth factor-2 (FGF-2), fibroblast growth factor receptor-1 (FGFR-1), β-catenin, epithelial cell adhesion molecule (EPCAM), Tenomodulin and scleraxis protein expressions. Pearson correlation analysis was applied to analyze the correlation between HSP72 expression and tendon adhesion. Results: At the 4^th week after surgery, we found no differences in the tendon adhesion scores or mRNA and protein expressions of HSP72 between the thermal preconditioning and control groups. However, after the 8^th week after surgery, the thermal preconditioning group had a lower tendon adhesion score and higher mRNA and protein expressions of HSP72 than the control group. During the same period, we found longer gliding distance and higher expression levels of FGF-2, FGFR-1, β-catenin, Tenomodulin and scleraxis, but lower EPCAM expression in the thermal preconditioning group. Pearson correlation analysis indicated that HSP72 mRNA and protein expression levels were negatively correlated with tendon adhesion. Conclusions: These findings provide evidence that thermal preconditioning may alleviate tendon adhesions via upregulation of HSP72 expression.

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Section: Discussionmentioning

confidence: 99%

Thermal Preconditioning May Prevent Tendon Adhesion by Up-Regulating HSP72 in Rats

Tan

et al. 2017

Cell Physiol Biochem

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“…We also screened an important cellular target of the nicotine metabolite cotinine, gelsolin, which may affect basic tumour transformation and metastasis processes, such as migration and apoptosis, through gelatine ( Nowak et al, 2015 ). Heat shock protein, on the other hand, is reported to be a major marker affected by cigarette smoke and is involved in signalling pathways associated with the cell cycle, cell death and inflammation ( Gál et al., 2011 ; Hulina-Tomašković et al., 2019 ; Parseghian, Hobson & Richieri, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Urine proteomic analysis of the rat e-cigarette model

Liu,

Shen,

Zhao

et al. 2023

PeerJ

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Background We were curious if the urinary proteome could reflect the effects of e-cigarettes on the organism. Methods Urine samples were collected from a rat e-cigarette model before, during, and after two weeks of e-cigarette smoking. Urine proteomes before and after smoking of each rat were compared individually, while the control group was set up to rule out differences caused by rat growth and development. Results Fetuin-B, a biomarker of chronic obstructive pulmonary disease (COPD), and annexin A2, which is recognized as a multiple tumour marker, were identified as differential proteins in five out of six smoking rats on day 3. To our surprise, odourant-binding proteins expressed in the olfactory epithelium were also found and were significantly upregulated. Pathways enriched by the differential proteins include the apelin signalling pathway, folate biosynthesis pathway, arachidonic acid metabolism, chemical carcinogenesis-DNA adducts and chemical carcinogenesis-reactive oxygen species. They have been reported to be associated with immune system, cardiovascular system, respiratory system, etc. Conclusions Urinary proteome could reflect the effects of e-cigarettes in rats.

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“…We also screened an important cellular target of the nicotine metabolite cotinine, gelsolin, which may affect basic processes of tumor transformation and metastasis, such as migration and apoptosis, through gelatin [41] . Heat shock protein, on the other hand, is reported to be a major marker affected by cigarette smoke and is involved in signaling pathways for the cell cycle and cell death and inflammation [42]- [44] .…”

Section: Discussionmentioning

confidence: 99%

Urine proteomic analysis of the rat e-cigarette model

Liu

Shen

Zhao

et al. 2022

Preprint

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Urinary proteomics was used to investigate the potential effects of e-cigarettes on the human body. In this study, a rat e-cigarette model was constructed by smoking for two weeks and urine samples before, during, and after e-cigarette smoking were collected. Urine proteomes before-after smoking of each rat were compared individually, while the control group was set up to rule out differences caused by rat growth and development. After smoking, the differential proteins produced by rats shows strong individual variation. Fetuin-B, a biomarker of COPD, and annexin A2, which is recognized as a multiple tumor marker, were identified as the differential proteins in five out of six smoking rats on day 3. To our surprise, odorant-binding proteins expressed in the olfactory epithelium were also found and were significantly upregulated. Pathways enriched by the differential proteins shows the evidence that smoking e-cigarettes affects the immune system, cardiovascular system, respiratory system, etc., which provides clues for further exploration of the mechanism of e-cigarettes on the human body.

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Targeted heat shock protein 72 for pulmonary cytoprotection

Cited by 13 publications

References 60 publications

Thermal Preconditioning May Prevent Tendon Adhesion by Up-Regulating HSP72 in Rats

Thermal Preconditioning May Prevent Tendon Adhesion by Up-Regulating HSP72 in Rats

Urine proteomic analysis of the rat e-cigarette model

Urine proteomic analysis of the rat e-cigarette model

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